Gallic acid inhibits migration and invasion in human osteosarcoma U-2 OS cells through suppressing the matrix metalloproteinase-2/-9, protein kinase B (PKB) and PKC signaling pathways

Liao, Ching Lung; Lai, Kuang Chi; Huang, An; Yang, Jai Sing; Lin, Jen Jyh; Wu, Shin Hwar; Wood, W. G.; Lin, Jaung Geng; Chung, Jing Gung

doi:10.1016/j.fct.2012.02.033

Cited by 114 publications

(88 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GBM 8401 cells (1.6x10 5 cells/well) were plated on 12-well tissue culture plates and incubated with 0, 2 and 4 µM PEITC or 500 µM TMZ for 24 and 48 h. The conditioned medium was collected and separated by electrophoresis on 10% SdS-PAGE with 0.2% gelatin (Sigma-Aldrich Corp.). The gels were soaked in 2.5% Triton X-100 in dH 2 O twice for a total of 60 min at 25˚C at the end of the electrophoresis, and they were incubated in substrate buffer (50 mM Tris hCl, 5 mM CaCl 2 , 0.02% NaN 3 and 1% Triton X-100, pH 8.0) at 37˚C for 18 h. Bands related to the enzyme activity of MMP-2 were visualized by negative staining using 0.2% Coomassie blue in 50% methanol and 10% acetic acid (20). The bands were evaluated by Image J software.…”

Section: Methodsmentioning

confidence: 99%

PEITC inhibits human brain glioblastoma GBM 8401 cell migration and invasion through the inhibition of uPA, Rho A, and Ras with inhibition of MMP-2, -7 and -9 gene expression

et al. 2015

Self Cite

View full text Add to dashboard Cite

Abstract. Glioblastoma is the most aggressive primary brain malignancy, and the efficacy of multimodality treatments remains unsatisfactory. Phenethyl isothiocyanate (PEITC), one member of the isothiocyanate family, was found to inhibit the migration and invasion of many types of human cancer cells. In our previous study, PEITC induced the apoptosis of human brain glioblastoma GBM 8401 cells through the extrinsic and intrinsic signaling pathways. In the present study, we first investigated the effects of PEITC on the migration and invasion of GBM 8401 cells. PEITC decreased the migration of GBM 8401 cells in a dose-dependent manner as determined from scratch wound healing and Transwell migration assays. The percentage of inhibition ranged from 46.89 to 15.75%, and from 27.80 to 7.31% after a 48-h treatment of PEITC as determined from the Transwell migration assay and invasion assay, respectively. The western blot analysis indicated that PEITC decreased the levels of proteins associated with migration and invasion, Ras, uPA, RhoA, GRB2, p-p38, p-JNK, p-ERK, p65, SOS1, MMP-2, MMP-9 and MMP-13, in a dose-dependent manner. Real-time PCR analyses revealed that PEITC reduced the mRNA levels of MMP-2, MMP-7, MMP-9 and RhoA in a dose-and time-dependent manner. PEITC exhibited potent anticancer activities through the inhibition of migration and invasion in the GBM 8401 cells. Our findings elucidate the possible molecular mechanisms and signaling pathways of the anti-metastatic effects of PEITC on human brain glioblastoma cells, and PEITC may be considered as a therapeutic agent. IntroductionGlioblastoma is the most aggressive primary brain malignancy with a median survival rate of 14.6 months from diagnosis in unselected patients, even following maximal, feasible surgical resection, radiotherapy and standard adjuvant temozolomide (TMZ) therapy (1). Only 0.4-0.5% of all GBM patients with extracranial metastasis has been reported, which may be attributable to the extremely shortened survival of these patients (2). Combining radiotherapy and TMZ provides better survival outcomes of glioblastoma patients than radiotherapy alone (3). Survival and recurrence are significantly associated with the extent of resection and residual volume (4). Gross total resection associated with survival improvement is not always possible as the preservation of neurological functions is necessary. The efficacy of current multimodality treatments including surgery, radiotherapy, chemotherapy for this tumor remains unsatisfactory.Phenethyl isothiocyanate (PEITC) is one of the most extensively studied isothiocyanates (5). PEITC can induce cell cycle arrest and apoptotic cell death in various tumor types (6-12). In our previous study, PEITC induced apoptosis through the extrinsic (death receptor) and intrinsic (mitochondrial) pathways, dysfunction of mitochondria and ROS-induced ER stress in GBM 8401 cells (13). PEITC displayed anti-metastatic PEITC inhibits human brain glioblastoma GBM 8401 cell migration and invasion through the inhibition of uPA,...

show abstract

Section: Methodsmentioning

confidence: 99%

PEITC inhibits human brain glioblastoma GBM 8401 cell migration and invasion through the inhibition of uPA, Rho A, and Ras with inhibition of MMP-2, -7 and -9 gene expression

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…F21 is composed of 68.49% gallic acid, and there are many studies that have reported the anti-cancer activity of this compound. Gallic acid can interfere with different stages of tumor development with the following activities: decreases the ornithine decarboxylase response linked to skin tumor promotion by 12-O-tetradecanoylphorbol-13-acetate (Gali et al, 1991); inhibits P815 cell metastasis to the liver (Ohno et al, 2001); inhibits activator protein-1 (AP-1) transcriptional activity (Maggi-Capeyron et al, 2001); suppresses tumor angiogenesis (Liu et al, 2006b); inhibits the growth of HeLa cervical cancer cells via apoptosis and/or necrosis (You et al, 2010); decreases cell viability, proliferation, invasion and tube formation of gliomas (Lu et al, 2010) by suppressing ADAM17, which may contribute to the inhibition of invasiveness through the inactivation of PI3K/Akt and Ras/MAPK signaling pathways; and inhibits migration and invasion in human osteosarcoma U-2 OS cells through suppressing the matrix metalloproteinase-2/-9, protein kinase B (PKB) and PKC signaling pathways (Liao et al, 2012). In the present study, the effect of gallic acid alone and the F21 fraction was investigated in human cervical cell lines (HeLa, SiHa, and C33A).…”

Section: 449 Cytotoxic Effects Of Phytophenolics From Caesalpinia MImentioning

confidence: 99%

Cytotoxic Effects of Phytophenolics from Caesalpinia mimosoides Lamk on Cervical Carcinoma Cell Lines through an Apoptotic Pathway

Palasap¹,

Limpaiboon²,

Boonsiri³

et al. 2014

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

“…Gallic acid (GA) has a biological activities such as antibacterial, antiviral, anti-inflammatory, antioxidant, antitumor (Liao et al 2012) and antidiabetic effects and reduces heart infarction incidence and oxidative liver damage. It received much attention because of its potent ability to scavenge ROS, such as superoxide anions, hydrogen peroxide, hydroxyl radicals and hypochlorous acid (Mansouri et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Preventive role of gallic acid on hepatic ischemia and reperfusion injury in rats

et al. 2014

View full text Add to dashboard Cite

There is little information about the hepatoprotective effects of gallic acid against ischemiareperfusion (I/R) damage. Animals were subjected to I/R. Gallic acid at doses of 50 and 100 mg/kg body weight (bw) were injected as a single dose prior to ischemia. Liver tissue homogenates were used for the measurement of malondialdehyde (MDA), catalase (CAT) and glutathione peroxidase (GPx) levels. At the same time alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were assayed in serum samples and compared statistically. While the ALT, AST, LDH activities and MDA levels were significantly increased, CAT and GPx activities significantly decreased in only I/Rinduced control rats compared to normal control rats (P \ 0.05). Treatment with gallic acid at a dose of 100 mg/kg bw significantly decreased the ALT, AST, LDH activities and MDA levels, and markedly increased activities of CAT and GPx in tissue homogenates compared to I/R-induced rats with no treatment group (P \ 0.05). In oxidative stress generated by hepatic ischemia-reperfusion, gallic acid contributes partially an alteration in the delicate balance between the scavenging capacity of antioxidant defense systems and free radicals in favour of the antioxidant defense systems in the body.

show abstract

Gallic acid inhibits migration and invasion in human osteosarcoma U-2 OS cells through suppressing the matrix metalloproteinase-2/-9, protein kinase B (PKB) and PKC signaling pathways

Cited by 114 publications

References 52 publications

PEITC inhibits human brain glioblastoma GBM 8401 cell migration and invasion through the inhibition of uPA, Rho A, and Ras with inhibition of MMP-2, -7 and -9 gene expression

PEITC inhibits human brain glioblastoma GBM 8401 cell migration and invasion through the inhibition of uPA, Rho A, and Ras with inhibition of MMP-2, -7 and -9 gene expression

Cytotoxic Effects of Phytophenolics from Caesalpinia mimosoides Lamk on Cervical Carcinoma Cell Lines through an Apoptotic Pathway

Preventive role of gallic acid on hepatic ischemia and reperfusion injury in rats

Contact Info

Product

Resources

About