Gallic acid-induced lung cancer cell death is related to glutathione depletion as well as reactive oxygen species increase

You, Bo; Park, Woo Hyun

doi:10.1016/j.tiv.2010.04.009

Cited by 137 publications

(88 citation statements)

References 34 publications

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“…Studies reported that it has higher sensitivity to kill cancer cells, compared to normal cells (Inoue et (You & Park, 2010). This finding was supported by earlier study by Sakaguchi et al (1998), where no cancer cell apoptosis and DNA fragmentation were noticed when intracellular Ca 2+ chelator (BAPTA-AM), calmodulin inhibitor (W-7) and radical scavengers (ascorbic acid, catalase, superoxide dismutase and thiol antioxidant n-acetyl-L-cysteine), were added into the culture.…”

Section: Apoptotic Death Of Pc-3 Cells Induced By Compoundsupporting

confidence: 65%

Bioactivity-Guided Isolation of Anticancer Agents from <i>Bauhinia Kockiana</i> Korth.

Chew¹,

Lim²,

Stanslas³

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

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Background: Flowers of Bauhinia kockiana were investigated for their anticancer properties. Methods: Gallic acid (1), and methyl gallate (2), were isolated via bioassay-directed isolation, and they exhibited anticancer properties towards several cancer cell lines, examined using MTT cell viability assay. Pyrogallol (3) was examined against the same cancer cell lines to deduce the bioactive functional group of the phenolic compounds. Results:The results showed that the phenolic compounds could exhibit moderate to weak cytotoxicity towards certain cell lines (GI 50 30 -86 µM), but were inactive towards DU145 prostate cancer cell (GI 50 > 100 µM). Conclusion: It was observed that pyrogallol moiety was one of the essential functional structures of the phenolic compounds in exhibiting anticancer activity. Also, the carboxyl group of compound 1 was also important in anticancer activity. Examination of the PC-3 cells treated with compound 1 using fluorescence microscopy showed that PC-3 cells were killed by apoptosis.

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Section: Apoptotic Death Of Pc-3 Cells Induced By Compoundsupporting

confidence: 65%

Bioactivity-Guided Isolation of Anticancer Agents from <i>Bauhinia Kockiana</i> Korth.

Chew¹,

Lim²,

Stanslas³

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

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“…Gallic acid (3,4,5-trihydroxybenzoic acid), a polyphenol present in several fruits, vegetables, and derived products, has been described as a powerful natural antioxidant that is capable of eliminating reactive oxygen species such as superoxide anions, hydrogen peroxide, and hydroxyl radicals (Polewski et al, 2002;Yen et al 2002). Regarding the use of the gallic acid, several studies have reported the anticancer, anti-angiogenic (Chen et al, 2009;Lu et al, 2010;You & Park, 2010;Hseu et al, 2011), and antimicrobial properties (Chanwitheesuk et al, 2007;). It is used in the pharmaceutical industry for trimethoprim production and dye manufacturing (Benerjee & Gargi, 2006).…”

Section: Resultsmentioning

confidence: 99%

Gallic acid as the major antioxidant in pequi (Caryocar brasiliense Camb.) fruit peel

Rocha

Melo

Paula

et al. 2015

Rev. bras. plantas med.

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ABSTRACT:In this study, ethanol-water extracts of pequi fruit peel were fractionated in order to identify and quantify the major antioxidant present in it. The fractions were subjected to liquid-liquid phase extraction and silica-gel column chromatography, and antioxidant activity was monitored using the 2,2-diphenyl-1-picrylhydrazyl radical-scavenging assay. The purity of the fractions was evaluated using thin-layer chromatography and high-performance liquid chromatography (HPLC). The substance with antioxidant property was identified through the analysis in a liquid chromatography-mass spectroscopy fragmentation and was quantified using HPLC. After the Silica-gel fractionation, it was identified a fraction with high antioxidant activity and purity, which contained gallic acid as the main compound. The gallic acid was found at the amount of 26.54 ± 1.13 mg/g of the dry mass of the pequi fruit peel. Because the quantifications were performed using crude ethanol-water extract, it was suspected that gallic acid was present in a free form. Thus, pequi fruit peel may serve as an attractive alternative of feedstock for natural antioxidant production. Moreover, the results obtained in this study emphasize the value of the pequi plant, and suggests improved opportunities for families that use this fruit`s products.Keywords: plant extracts, fractionation, natural products, antioxidants RESUMO: Ácido gálico como principal antioxidante da casca do fruto do pequi (Caryocar brasiliense Camb.). Extratos hidroetanólicos da casca do fruto do pequi foram fracionados a fim de identificar e quantificar o principal antioxidante presente. Frações do extrato foram submetidas ao particionamento líquido-líquido e fracionamento em coluna de sílica gel. As atividades antioxidantes das frações foram monitoradas usando ensaio de redução do radical 2,2-difenil-1-picrilhidrazila e a pureza das frações foi avaliada em cromatografia de camada delgada e cromatografia líquida de alta eficiência (CLAE). A substância com propriedades antioxidantes foi identificada através da análise em sistema de cromatografia líquida associada à espectrometria de massas e foi quantificada em HPLC. Após o fracionamento identificou-se uma fração com alta atividade antioxidante e pureza, contendo ácido gálico como o composto principal. Ácido gálico foi encontrado em concentrações de 26,54 ± 1,13 mg/g de massa seca. Devido às quantificações terem sido realizadas no extrato hidroetanólico bruto, acredita-se que o ácido gálico esteja presente na forma livre. Assim, a casca do fruto pequi pode servir como interessante alternativa de matéria prima para a produção desse antioxidante natural. Além disso, esse resultado enfatiza o valor da planta do pequi e sugere oportunidades para as famílias que utilizam produtos de pequi.Palavras-chave: extratos de plantas, fracionamento, produtos naturais, antioxidantes

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“…In contradiction to this fi nding, Ximenes et al (29) suggested that gallic acid is prooxidative in cellular systems and could exacerbate the drug-induced prooxidative damage in human erythrocytes. The prooxidative effect of gallic acid at the molecular level (30) and oxidative-based induction of cell death in cancer cells (31,32) have also been documented. Furthermore, the in vitro induction of cancer cells through the generation of ROS has also been linked to the depletion of antioxidant defences (32).…”

Section: Resultsmentioning

confidence: 99%

“…The prooxidative effect of gallic acid at the molecular level (30) and oxidative-based induction of cell death in cancer cells (31,32) have also been documented. Furthermore, the in vitro induction of cancer cells through the generation of ROS has also been linked to the depletion of antioxidant defences (32). Previous studies have also suggested that fl uoride intoxication leads to excessive ROS generation, and inhibition of endogenous antioxidant enzymatic activities (33), increased lipid peroxidation, and cell injury (34).…”

Section: Resultsmentioning

confidence: 99%

In Vivo Protective Effects of Gallic Acid Isolated from Peltiphyllum Peltatum Against Sodium Fluoride-Induced Oxidative Stress in Rat Erythrocytes

Nabavi

Habtemariam

Sureda

et al. 2013

Archives of Industrial Hygiene and Toxicology

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Gallic acid has been identifi ed as an antioxidant component of the edible and medicinal plant Peltiphyllum peltatum. The present study examined its potential protective role against sodium fl uoride (NaF)-induced oxidative stress in rat erythrocytes. Oxidative stress was induced by NaF administration through drinking water (1030.675 mg m -3 for one week). Gallic acid at 10 mg kg -1 and 20 mg kg -1 and vitamin C for positive controls (10 mg kg -1 ) were administered daily intraperitoneally for one week prior to NaF administration. Thiobarbituric acid reactive substances, antioxidant enzyme activities (superoxide dismutase and catalase), and the level of reduced glutathione were evaluated in rat erythrocytes. Lipid peroxidation in NaF-exposed rats signifi cantly increased (by 88.8 %) when compared to the control group (p<0.05). Pre-treatment with gallic acid suppressed lipid peroxidation in erythrocytes in a dose-dependent manner. Catalase and superoxide dismutase enzyme activities and glutathione levels were reduced by NaF intoxication by 54.4 %, 63.69 %, and 42 % (p<0.001; vs. untreated control group), respectively. Pre-treatment with gallic acid or vitamin C signifi cantly attenuated the deleterious effects. Gallic acid isolated from Peltiphyllum peltatum and vitamin C mitigated the NaF-induced oxidative stress in rat erythrocytes.

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Gallic acid-induced lung cancer cell death is related to glutathione depletion as well as reactive oxygen species increase

Cited by 137 publications

References 34 publications

Bioactivity-Guided Isolation of Anticancer Agents from <i>Bauhinia Kockiana</i> Korth.

Bioactivity-Guided Isolation of Anticancer Agents from <i>Bauhinia Kockiana</i> Korth.

Gallic acid as the major antioxidant in pequi (Caryocar brasiliense Camb.) fruit peel

In Vivo Protective Effects of Gallic Acid Isolated from Peltiphyllum Peltatum Against Sodium Fluoride-Induced Oxidative Stress in Rat Erythrocytes

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