Gallic Acid Enhancement of Gold Nanoparticle Anticancer Activity in Cervical Cancer Cells

Daduang, Jureerut; Palasap, Adisak; Daduang, Sakda; Boonsiri, Patcharee; Suwannalert, Prasit; Limpaiboon, Temduang

doi:10.7314/apjcp.2015.16.1.169

Cited by 67 publications

(34 citation statements)

References 34 publications

(30 reference statements)

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“…The biocompatible nature of ap-AuNPs is shown by its nontoxicity towards normal epidermoid cells (HaCat). 48 The viability results are comparable to that of A431 cells indicating that the ap-AuNPs exhibit comparable cytotoxicity towards these two types of epidermoid squamous carcinoma cell lines. …”

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confidence: 66%

Apigenin mediated gold nanoparticle synthesis and their anti-cancer effect on human epidermoid carcinoma (A431) cells

et al. 2015

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We report that the flavonoid, apigenin (4', 5, 7,-trihydroxyflavone) is able to form apigenin linked gold nanoparticles (ap-AuNPs) at RT with apigenin itself acting as the stabilizing agent.The synthesized ap-AuNPs have been characterised by UV-Visible spectroscopy, HR-TEM, DLS, FTIR and TGA analyses. The biocompatible nature of ap-AuNPs is shown by its nontoxicity towards normal epidermoid cells (HaCat). Additionally, it is shown to exhibit anticancer activity towards epidermoid squamous carcinoma cells (A431). The uptake of ap-AuNPs into A431 cells is seen by TEM. Apoptosis induced by ap-AuNPs in these cells is observed through EtBr/AO and DAPI staining. Flow cytometric results also reveal the occurrence of early apoptotic cells, late apoptotic cells and the presence of sub-G1 population. DNA prepared from ap-AuNPs treated A-431 cells reveals a ladder like pattern in agarose gel electrophoresis indicating oligonucleosomal cleavage which is a hallmark of apoptosis. The ap-AuNPs also inhibit angiogenesis as shown by Chick chorioallantoic membrane (CAM) assay. Taking the results together, we believe that ap-AuNPs show promise in treatment of skin cancer. The ap-AuNPs also have cytotoxic potential against the human cervical squamous cell carcinoma cell line -SiHa.indicating that almost a four-fold increase in capacity to kill the cancer cells is realized by the ap-AuNPs conjugation.The effect of ap-AuNPs on SiHa cells has also been studied. The results show a concentration and time dependent decrease in viability. On treatment with 200 µg / mL of ap-AuNPs for 24 h, the viability is found to be 52.7 ± 2.6 %, which further decreases to 44.2 ± 3.1 % and 31.8 ± 1.1 % after 48 h and 72 h respectively (Fig. S5a). Gallic acid conjugated gold nanoparticles (150µM) are reported to induce cell death in SiHa cells. 48 The viability results are comparable to that of A431 cells indicating that the ap-AuNPs exhibit comparable cytotoxicity towards these two types of epidermoid squamous carcinoma cell lines. Microscopy Analysis EtBr/AO and DAPI stainingThe EtBr/AO staining of HaCat and A431 cells treated with different concentrations (50, 100, 150 and 200 µg / mL) of ap-AuNPs for 48 h has been carried out. Fig. 5a shows that the control and treated HaCat cells possess similar morphology and fluoresce green, which indicates that they are viable. No significant difference is observed even in the presence of a higher concentration of ap-AuNPs (200 µg / mL). But at the same time, A431 cells treated at a concentration of 50 µg / mL of ap-AuNPs show the presence of a few dead cells (red fluorescence). The initial stage of membrane damage and reddish-orange colour stained cells are observed in the presence of 100 µg / mL of ap-AuNPs. The loss of membrane integrity with most of the cells fluorescing red post-treatment at concentrations of 150 and 200 µg / mL of ap-AuNPs has also been observed. Under these conditions, some cells are also found to detach from the plate (Fig. 5b).The concentration of Au in the ap-AuNPs was determined by atom...

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Apigenin mediated gold nanoparticle synthesis and their anti-cancer effect on human epidermoid carcinoma (A431) cells

et al. 2015

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“…The IC 50 concentration of polysaccharide-coated nanoparticles at IC 50 dose of 70.3 ± 1.2 μg/ml acts effectively against breast cancer cell line MCF7 and 48.9 ± 1.8 μg/ml concentration against leukemia cell line K562 [8]. The high concentration of AuNPs showed excellent cytotoxic activity against cervical cancer cell lines and also did not affect normal cells [41]. Previous reports stated that 85 μg/ml concentration of AuNPs increases the apoptotic morphological changes in treated HepG2 cells, while the untreated HepG2 cells were clustered, healthy, and viable [42].…”

Section: Discussionmentioning

confidence: 98%

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

et al. 2017

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The present study reports the chemical synthesis of gold nanoparticles (AuNPs) using sodium borohydride as a reducing and capping agent. The various temperatures and time intervals were investigated for controlled formation of AuNPs. The characterization of AuNPs was studied using UV-vis spectrophotometer, transmission electron microscope (TEM), selected area diffraction (SAED), energy-dispersive X-ray spectrophotometer (Edx), dynamic light scattering (DLS), zeta potential, and X-ray powder diffraction (XRD), and results indicated that AuNPs were spheroid in shape and cubic lattice and face centered in nature with 29 nm in size. AuNPs have potential antimicrobial activity against selected human clinical pathogens. IC 50 concentration of AuNPs was determined as 20 μg/ml against non-small cell lung cancer (NSCLC) cell lines using MTT assay. The morphological and apoptosis effects of AuNPs on NSCLC were investigated using acridine orange/ethidium bromide (AO/EB), 2′,7′-dichlorofluorescein-diacetate (DCFH-DA), rhodamine 123, and 4′,6-diamidino-2-phenylindole (DAPI) staining method, and results indicated that AuNPs have potential anticancer activity against NSCLC cell lines. The anticancer activity of AuNPs is that it has less toxicity against human HBL100 normal cells. These anticancer potentials of AuNPs were compared with the standard drug cisplatin. Moreover, after 24 h exposure, AuNPs arrest the cell cycle at G2/M phase in A549 cells. These findings suggested that AuNPs could act as a potential anticancer agent against lung cancer.

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“…Many researchers have recently reported the biosynthesis of AuNPs using phytochemicals, which have become an interesting area of research in the biomedical field by producing safe nanoparticles that can be used in medical applications. A plethora of research studies in which AuNPs are synthesized using plant extracts and pure phytochemicals have been reported, with a few of them being mulberry leaf extract [3], Achyranthes asparalin seed extract [10], Solanum nigrum leaf extract [5], Ananas comosus fruit extract [11], Elettaria cardamomum aqueous extract [12], Stevia rebaudiana leaf extract [13], Buta monosperma leaf extract [14], gallic acid [15] Epigallocatechin-3-gallate (EGCG) [16] and Hesperidin [17]. Although there are many reports, the fabrication methods of AuNPs using phytochemicals and their application in cancer therapy are still sparse.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Synthesis and Characterization of Bioactive Gold Nanoparticles using Hypaphorine and their Cytotoxic Activity against HCT 116 Human Colorectal Cancer Cells

2016

International Conference on Advances in Science, Engineering, Technology and Natural Resources (ICASETNR-16) Nov. 24-25, 2016 P

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Abstract-Green synthesised gold nanoparticles (AuNPs) have received intense interest owing to their remarkable biomedical applications, particularly in cancer therapy. The plant mediated synthesis of AuNPs is of interest to biologists and chemists due to non-toxic, rapid, and convenient benefits over chemically and physically synthesised counterparts. However, the fabrication methods of AuNPs using pure phytochemicals and their application in cancer therapy are sparse. In the present study we used hypaphorine to successfully reduce gold salt to corresponding AuNPs in a single step; a process that fulfils all criteria of green nanotechnology. Characterization of hypaphorine capped AuNPs by UV-Vis spectroscopy; Fourier transform infrared spectroscopy; Dynamic light scattering and Transmission electron microscopy revealed that the synthesised AuNPs were spherically shaped with size range of 20-60 nm and polydispersed. The synthesised hypaphorine-capped AuNPs showed excellent in vitro cytotoxic activity against HCT 116 colorectal cancer cells and little or no toxicity to normal BHK 21 cells. The results of this study demonstrate the feasibility of using hypaphorine for the synthesis of AuNPs to fulfil the growing demand for rapid, convenient and non-hazardous AuNPs. Also the study provides guidance towards the development of alternative treatment of cancer therapy using biologically synthesised AuNPs. However, there is a need for further clinical studies to ascertain their potential as highly active anticancer agents. To the best of our knowledge, there is no report available on the synthesis of AuNPs using pure hypaphorine and no such articles is available to describe their cytotoxic effect in HCT 116 human colorectal cancer cells.

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Gallic Acid Enhancement of Gold Nanoparticle Anticancer Activity in Cervical Cancer Cells

Cited by 67 publications

References 34 publications

Apigenin mediated gold nanoparticle synthesis and their anti-cancer effect on human epidermoid carcinoma (A431) cells

Apigenin mediated gold nanoparticle synthesis and their anti-cancer effect on human epidermoid carcinoma (A431) cells

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

Phytochemical Synthesis and Characterization of Bioactive Gold Nanoparticles using Hypaphorine and their Cytotoxic Activity against HCT 116 Human Colorectal Cancer Cells

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