GABAergic Innervation of Oxytocin Neurons in the Paraventricular Nucleus of the Rat Hypothalamus as Revealed by Double Labeling Immunoelectron-Microscopy

Shioda, Seiji; Hori, Toshiko; Nakai, Yasumitsu

doi:10.15369/sujms1989.3.167

Cited by 2 publications

(2 citation statements)

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“…The serotonergic innervation of SON and PVN appears to derive mainly from the midbrain nuclei of the raphe (25), i s most dense in the dorsal, predominantly oxytocincrgic portion of the SON, and in this region serotonergic axon terminals make synaptic contact with oxytocin cell bodies (26). In the rat, serotonin i s inhibitory to both oxytocin and vasopressin neurons (27,28).…”

Section: Discussionmentioning

confidence: 99%

Noradrenaline, Dopamine and Serotonin Release in the Paraventricular and Supraoptic Nuclei of the Rat in Response to Intravenous Cholecystokinin Injections

Kendrick

Leng

Higuchi

1991

J Neuroendocrinology

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Microdialysis sampling was used to measure noradrenaline, dopamine and serotonin release in the supraoptic and paraventricular nuclei of urethane-anaesthetized rats following intravenous injection of 20 pglkg cholecystokinin. This dose of cholecystokinin stimulates oxytocin release from the posterior pituitary, while slightly inhibiting vasopressin release. Dialysis probes were placed in the paraventricular nucleus, and into dorsal or ventral regions of the supraoptic nucleus. Samples were collected at 10-min intervals in each animal before, during and after two injections of cholecystokinin, and following a control injection of 0.9% NaCI.The injections of cholecystokinin stimulated significant increases in the concentrations of noradrenaline, dopamine and serotonin in the paraventricular nucleus and of noradrenaline and serotonin in the dorsal supraoptic nucleus region. Conversely, in the ventral supraoptic nucleus region a significant reduction in noradrenaline release was observed, but dopamine and serotonin concentrations were not significantly affected. The control injections did not alter noradrenaline, dopamine or serotonin release.In the rat, peripheral injections of cholecystokinin octapeptide (CCK) result in a transient increase in the plasma concentration of oxytocin and a small reduction in plasma vasopressin concentration (1-3). Systemic injections of CCK increase the electrical activity of magnocellular oxytocin cells in the hypothalamus (4), and this action of CCK is apparently mediated via activation of the gastric vagus (2). Although the supraoptic nucleus (SON) contains a high density of specific binding sites for CCK (5), and SON neurons in vitro are excited by bath application of CCK (6), the SON and the paraventricular nucleus (PVN) are within the blood-brain barrier, which in general excludes blood-borne peptides. Direct application of CCK can induce the release of both vasopressin and oxytocin from the isolated neural lobe in vitro (7), but since gastric vagotomy abolishes the oxytocin response to systemic CCK (2)-and lesions in the vicinity of the area postrema attenuate the response (8), it is likely that the principal action of systemically-administered CCK is mediated via the nucleus tractus solitarius, which is adjacent to the area postrema, receives an extensive input from the dorsal motor nucleus of the vagus, and projects both directly, and indirectly via the ventrolateral medulla, to the SON and PVN (9). In particular, there are strong noradrenergic projections from the AI cell group in the ventrolateral medulla, and from the A2 cell group in the nucleus tractus solitarius to the vicinity of the SON and PVN (9-1 5). Noradrenaline is predominantly excitatory to both oxytocin and vasopressin magnocellular neurons (13-16). We have previously shown, using microdialysis sampling, that noradrenaline and serotonin concentrations are increased in the area of the SON following haemorrhage (17). In the present study we used microdialysis sampling followed by HPLC with electrochemica...

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Section: Discussionmentioning

confidence: 99%

Noradrenaline, Dopamine and Serotonin Release in the Paraventricular and Supraoptic Nuclei of the Rat in Response to Intravenous Cholecystokinin Injections

Kendrick

Leng

Higuchi

1991

J Neuroendocrinology

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“…On the other hand, the isolated GVs were fixed with paraforrnaldehydeglutaraldehyde, post-fixed osmium tetroxide, and infiltrated in LR white resin as described above and sectioned. The sections were etched with saturated sodium metaperiodate for 1 hr (Shioda et al 1989), washed and then incubated with the GV-antiserum.…”

Section: Immunocytochemistrymentioning

confidence: 99%

Immunocytochemical and chemical analyses of Golgi vesicles isolated from the germinated pollen ofCamellia japonica

et al. 1998

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As a first step towards studying the biochemical relationship between Golgi vesicles (GVs) and tube wall components, isolation of GVs from the growing pollen tubes of Came/I/a japon/ca was attempted using a cenMfugaUon method with mannitoL The isolated GV was IdenU11ed ultrastructurally and immunocytochemically.The main components of the GV were proteins and carbohydrates. The main monosacchaddes of GV polysaccharicles were galactose, arabinose and uronic acid, and pectins and arablnogalactan proteins also were detected immunochemically. An antiserum against the isolated GVs reacted with the outer layer of the pollen tube wall and the inline layers of the grain wall as well as the in s/tu GVs in the pollen tube and the grain cytoplasm. We have thus successfully isolated GVs and shown mat they contain pec'dc substances and arabinogalactan proteins which contribute to formation of the pollen tube primary wall.

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GABAergic Innervation of Oxytocin Neurons in the Paraventricular Nucleus of the Rat Hypothalamus as Revealed by Double Labeling Immunoelectron-Microscopy

Cited by 2 publications

References 28 publications

Noradrenaline, Dopamine and Serotonin Release in the Paraventricular and Supraoptic Nuclei of the Rat in Response to Intravenous Cholecystokinin Injections

Noradrenaline, Dopamine and Serotonin Release in the Paraventricular and Supraoptic Nuclei of the Rat in Response to Intravenous Cholecystokinin Injections

Immunocytochemical and chemical analyses of Golgi vesicles isolated from the germinated pollen ofCamellia japonica

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