As a first step towards studying the biochemical relationship between Golgi vesicles (GVs) and tube wall components, isolation of GVs from the growing pollen tubes of Came/I/a japon/ca was attempted using a cenMfugaUon method with mannitoL The isolated GV was IdenU11ed ultrastructurally and immunocytochemically.The main components of the GV were proteins and carbohydrates. The main monosacchaddes of GV polysaccharicles were galactose, arabinose and uronic acid, and pectins and arablnogalactan proteins also were detected immunochemically. An antiserum against the isolated GVs reacted with the outer layer of the pollen tube wall and the inline layers of the grain wall as well as the in s/tu GVs in the pollen tube and the grain cytoplasm. We have thus successfully isolated GVs and shown mat they contain pec'dc substances and arabinogalactan proteins which contribute to formation of the pollen tube primary wall.
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