G-quadruplex microspheres-based optical RNA biosensor for arthropod-borne virus pathogen detection: A proof-of-concept with dengue serotype 2

Jamaluddin, Nur Diyana; Mazlan, Nur-Fadhilah; Tan, Ling Ling; Yusof, Nurul Yuziana Mohd; Khalid, Bahariah

doi:10.1016/j.ijbiomac.2021.12.047

Cited by 6 publications

(5 citation statements)

References 49 publications

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“…Researchers are also developing more precise ( Adesnik and Abdeladim, 2021 ) and less invasive optogenetic devices, such as SOUL ( Gong et al, 2020 ). At the same time, optogenetics has strong compatibility, it can be used to study a variety of diseases, such as diabetes ( Li et al, 2021 ), inflammation ( Baumschlager and Khammash, 2021 ; Bhat et al, 2021 ; Dos Santos et al, 2021 ; Jamaluddin et al, 2021 ; Michoud et al, 2021 ; Santos et al, 2021 ; Senok et al, 2021 ), tumors ( Kim et al, 2017 ; Adampourezare et al, 2021 ; Esmaeili et al, 2022 ), depression ( Hare et al, 2019 ), epilepsy ( Zhang and Wang, 2021 ) and so on. And in 2020, 45 laboratories around the world integrated all optogenetics resources and create an optogenetics experimental database ( Tremblay et al, 2020 ).…”

Section: Conclusion Challenges and Perspectivesmentioning

confidence: 99%

The Roles of Optogenetics and Technology in Neurobiology: A Review

Chen

Liang

et al. 2022

Front. Aging Neurosci.

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Optogenetic is a technique that combines optics and genetics to control specific neurons. This technique usually uses adenoviruses that encode photosensitive protein. The adenovirus may concentrate in a specific neural region. By shining light on the target nerve region, the photosensitive protein encoded by the adenovirus is controlled. Photosensitive proteins controlled by light can selectively allow ions inside and outside the cell membrane to pass through, resulting in inhibition or activation effects. Due to the high precision and minimally invasive, optogenetics has achieved good results in many fields, especially in the field of neuron functions and neural circuits. Significant advances have also been made in the study of many clinical diseases. This review focuses on the research of optogenetics in the field of neurobiology. These include how to use optogenetics to control nerve cells, study neural circuits, and treat diseases by changing the state of neurons. We hoped that this review will give a comprehensive understanding of the progress of optogenetics in the field of neurobiology.

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Section: Conclusion Challenges and Perspectivesmentioning

confidence: 99%

The Roles of Optogenetics and Technology in Neurobiology: A Review

Chen

Liang

et al. 2022

Front. Aging Neurosci.

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“…Despite its apparent simplicity, the genomic organization of RNA viruses hinders some level of complexity, often correlated with the virus reproductive capacity and infectivity. 16–19 RNA and DNA viruses, including flaviviruses, 20 Zika, 21 dengue, 22 and human immunodeficiency viruses (HIV), 23 possess putative guanine quadruplex sequences (PQSs). 24,25 These G-rich sequences are associated with crucial regulatory roles, especially in the framework of gene expression.…”

Section: Introductionmentioning

confidence: 99%

Resolving a guanine-quadruplex structure in the SARS-CoV-2 genome through circular dichroism and multiscale molecular modeling

D'Anna,

Miclot,

Bignon

et al. 2023

Chem. Sci.

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“…[13][14][15] Despite its apparent simplicity, the genomic organization of RNA viruses hinders some level of complexity, often correlated with the virus reproductive capacity and infectivity. [16][17][18][19] RNA and DNA viruses, including flaviviruses, 20 Zika, 21 Dengue, 22 and human immunodeficiency viruses (HIV), 23 possess putative guanine quadruplex sequences (PQS). 24,25 These G-rich sequences are associated to crucial regulatory roles, especially in the framework of gene expression.…”

Section: Introductionmentioning

confidence: 99%

Resolving a Guanine-Quadruplex Structure in the SARS-CoV-2 Genome through Circular Dichroism and Multiscale Molecular Modeling

D'Anna¹,

Miclot

Bignon

et al. 2023

Preprint

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The genome of SARS-CoV-2 coronavirus is made up of a single-stranded RNA fragment that can assume a specific secondary structure, whose stability can influence the virus ability to reproduce. Recent studies have identified putative guanine quadruplex sequences in SARS-CoV-2 genome fragments that are involved in coding for both structural and non-structural proteins. In this contribution, we focus on a specific G-rich sequence referred as RG-2, which codes for the non-structural protein 10 (Nsp10) and assumes a parallel guanine-quadruplex (G4) arrangement. We provide the secondary structure of the RG-2 G4 at atomistic resolution by molecular modeling and simulation, validated by the superposition of experimental and calculated electronic circular dichroism spectrum. Through both experimental and simulation approaches, we have demonstrated that pyridostatin (PDS), a widely recognized G4 binder, can bind to and stabilize RG-2 G4 more strongly than RG-1, another G4 forming sequence that was previously proposed as a potential target for antiviral drug candidates. Overall, this study highlights RG-2 as a valuable target to inhibit the translation and replication of SARS-CoV-2 paving the way towards original therapeutic approaches against emerging RNA viruses.

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G-quadruplex microspheres-based optical RNA biosensor for arthropod-borne virus pathogen detection: A proof-of-concept with dengue serotype 2

Cited by 6 publications

References 49 publications

The Roles of Optogenetics and Technology in Neurobiology: A Review

The Roles of Optogenetics and Technology in Neurobiology: A Review

Resolving a guanine-quadruplex structure in the SARS-CoV-2 genome through circular dichroism and multiscale molecular modeling

Resolving a Guanine-Quadruplex Structure in the SARS-CoV-2 Genome through Circular Dichroism and Multiscale Molecular Modeling

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