G Protein-Coupled Glutamate and GABA Receptors Form Complexes and Mutually Modulate Their Signals

Sakairi, Hakushun; Kamikubo, Yuji; Abe, Masayoshi; Ikeda, Keisuke; Ichiki, Arata; Tabata, Toshihide; Kano, Masanobu; Sakurai, Takashi

doi:10.1021/acschemneuro.9b00599

Cited by 7 publications

(6 citation statements)

References 52 publications

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“…Bees colonized with strain W8111 showed decreased inclusion rates of 5’ alternative start site and four different retained intron events. These results suggested that the glutamate receptor might be regulated by GABA in the brain [ 61 ]. Moreover, the colonization of specific gut strains affects AS of brain genes, which regulates the production of isoforms of the mGluR gene.…”

Section: Resultsmentioning

confidence: 99%

Honey bee genetics shape the strain-level structure of gut microbiota in social transmission

Lang

et al. 2021

Microbiome

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Background Honey bee gut microbiota transmitted via social interactions are beneficial to the host health. Although the microbial community is relatively stable, individual variations and high strain-level diversity have been detected across honey bees. Although the bee gut microbiota structure is influenced by environmental factors, the heritability of the gut members and the contribution of the host genetics remains elusive. Considering bees within a colony are not readily genetically identical due to the polyandry of the queen, we hypothesize that the microbiota structure can be shaped by host genetics. Results We used shotgun metagenomics to simultaneously profile the microbiota and host genotypes of bees from hives of four different subspecies. Gut composition is more distant between genetically different bees at both phylotype- and “sequence-discrete population” levels. We then performed a successive passaging experiment within colonies of hybrid bees generated by artificial insemination, which revealed that the microbial composition dramatically shifts across batches of bees during the social transmission. Specifically, different strains from the phylotype of Snodgrassella alvi are preferentially selected by genetically varied hosts, and strains from different hosts show a remarkably biased distribution of single-nucleotide polymorphism in the Type IV pili loci. Genome-wide association analysis identified that the relative abundance of a cluster of Bifidobacterium strains is associated with the host glutamate receptor gene specifically expressed in the bee brain. Finally, mono-colonization of Bifidobacterium with a specific polysaccharide utilization locus impacts the alternative splicing of the gluR-B gene, which is associated with an increased GABA level in the brain. Conclusions Our results indicated that host genetics influence the bee gut composition and suggest a gut-brain connection implicated in the gut bacterial strain preference. Honey bees have been used extensively as a model organism for social behaviors, genetics, and the gut microbiome. Further identification of host genetic function as a shaping force of microbial structure will advance our understanding of the host-microbe interactions.

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Section: Resultsmentioning

confidence: 99%

Honey bee genetics shape the strain-level structure of gut microbiota in social transmission

Lang

et al. 2021

Microbiome

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“…The treated slices were incubated with a primary antibody against NeuN (1:200 dilution), GFAP (1:200), and Iba1 (1:100) at 4˚C for 24 h. Slices treated with primary antibody and washed 5 times with PBS were incubated with a secondary antibody conjugated with Alexa Fluor 488 or 594 (1:400 dilution) at 24˚C for 2-2.5 h 21 . The slices were examined using an Olympus IX71 (Olympus, Tokyo, Japan) with Orca-ER cooled CCD camera (Hamamatsu Photonics, Hamamatsu, Japan) or a Leica SP5/TCS confocal laser scanning microscope (Leica Microsystems, Wetzlar, Germany) 23 .…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Ex-Vivo Analysis Platforms for Amyloid Precursor Protein Cleavage.

Kamikubo

Hao

Niisato

et al. 2021

Preprint

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Alzheimer’s disease (AD) is a progressive neurodegenerative brain disorder and the most common cause of dementia in the elderly. Large numbers of senile plaques, neurofibrillary tangles, and cerebral atrophy are characteristic features of AD. The main component of senile plaques is amyloid β peptide (Aβ), derive from the amyloid precursor protein (APP). AD has been extensively studied using cell line, primary culture of neural cells, and animal models, however, some discrepancy is observed in these results. Dissociated cultures have lost the tissue architecture of the brain including neural circuits, glial cells, and the extracellular matrix. Animal models require lengthy animal experiments and laborious monitoring of multiple parameters following manipulations. Therefore, it is necessary to connect these experimental models to understand the pathology of AD. In order to analyze long-term neuronal development and plasticity, and progressive neurodegenerative disease, experimental platform amenable to continuous observation and experimental manipulation is required. In this report, we provide a practical method to slice and cultivate rodent hippocampus to consecutively investigate the cleavage of APP and the secretion of Aβ as an ex vivo model.

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“…Dataset S3), suggesting that the glutamate receptor might also be regulated by GABA in the brain [60]. In addition, we detected the alternative splicing (AS) events in gluR-B, and rMATS analysis [47] indicated that gluR-B exhibited differential patterns of AS events between MF and MC bees.…”

Section: Bifidobacterium Alters Gene Expression and Alternative Splicmentioning

confidence: 88%

Honeybee genetics shape the strain-level structure of gut microbiota in social transmission

Lang

et al. 2020

Preprint

View full text Add to dashboard Cite

Honeybee gut microbiota transmitted via social interactions are beneficial to the host health. Although the microbial community is relatively stable, individual variations and high strain-level diversity have been detected across honeybees. Although the bee gut microbiota structure is influenced by environmental factors, the heritability of the gut members and the contribution of the host genetics remains elusive. Considering bees within a colony are not readily genetically identical due to the polyandry of queen, we hypothesize that the microbiota structure can be shaped by host genetics. We used shotgun metagenomics to simultaneously profile the microbiota and host genotypes of individuals from hives of four different subspecies. Gut composition is more distant between genetically different bees at both phylotype- and “sequence-discrete population”-level. We then performed a successive passaging experiment within colonies of hybrid bees generated by artificial insemination, which revealed that the microbial composition dramatically shifts across batches of bees during the social transmission. Specifically, different strains from the phylotype of Snodgrassella alvi are preferentially selected by genetically varied hosts, and strains from different hosts show a remarkably biased distribution of single-nucleotide polymorphism in the Type IV pili loci. A genome-wide association analysis identified that the relative abundance of a cluster of Bifidobacterium strains is associated with the host glutamate receptor gene that is specifically expressed in the bee brain. Finally, mono-colonization of Bifidobacterium with a specific polysaccharide utilization locus impacts the expression and alternative splicing of the gluR-B gene, which is associated with an altered circulating metabolomic profile. Our results indicated that host genetics influence the bee gut composition, and suggest a gut-brain connection implicated in the gut bacterial strain preference. Honeybees have been used extensively as a model organism for social behaviors, genetics, and gut microbiome. Further identification of host genetic function as shaping force of microbial structure will advance our understanding of the host-microbe interactions.

show abstract

G Protein-Coupled Glutamate and GABA Receptors Form Complexes and Mutually Modulate Their Signals

Cited by 7 publications

References 52 publications

Honey bee genetics shape the strain-level structure of gut microbiota in social transmission

Honey bee genetics shape the strain-level structure of gut microbiota in social transmission

Ex-Vivo Analysis Platforms for Amyloid Precursor Protein Cleavage.

Honeybee genetics shape the strain-level structure of gut microbiota in social transmission

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