Future perspective of nanoparticle interaction‐assisted laser desorption/ionization mass spectrometry for rapid, simple, direct and sensitive detection of microorganisms

Abstract

The introduction of nanoparticles into mass spectrometric research greatly influenced the applicability of this technique into various omics. Surface-modified or functionalized nanoparticles (NPs) have recently extended the use of mass spectrometry into microorganism research. We survey the application of unmodified NPs, for microorganism research, on the basis of our expertise in this area within the recent years in this decade. The use of unmodified NPs in mass spectrometry, especially with respect to microorganisms, is an untreaded research area, which we have ventured to probe and have been fruitful. On the basis of our experience, we provide an insight into the principle behind the use of unmodified NPs and provide guidelines to be followed to obtain significant results. We also brief the current scenario of nanoparticle interaction-assisted laser desorption/ionization mass spectrometry (NPILDI-MS) for rapid, simple, direct and sensitive detection of microorganisms on the basis of our past and present reports, quoting examples of successful application of this technique. Finally, we address the future of the NPILDI-MS technique and the tools needed to reach those visions.

A comparative study on the mode of interaction of different nanoparticles during MALDI‐MS of bacterial cells

“…Therefore, the current approach was applied for further experiments. We have previously reported the superiority of the proposed method in a review . In this paper, we provide the evidence showing the superiority of this method during bacterial studies using MALDI‐MS.…”

Analyses of functional polymer-modified nanoparticles for protein sensing by surface-assisted laser desorption/ionization mass spectrometry coupled with HgTe nanomatrices

“…Nanoparticle (NP)-and nanosubstrate-based surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) techniques are being used more widely to mediate desorption processes; they operate with several attractive features, including ready preparation of homogeneous samples, excellent analyte-loading capacity, high salt tolerance, and low background noise in the low mass-to-charge region [15][16][17][18][19][20][21]. The main shortcoming when conducting SALDI-MS with NPs as the matrix for the analysis of biomacromolecules and polymers is inefficient desorption and ionization, due to the strong nonspecific interactions between large molecules and NPs [22,23].…”

Negative ion laser desorption/ionization time‐of‐flight mass spectrometric analysis of small molecules by using nanostructured substrate as matrices

“…In the past two decades, SALDI‐TOF MS serving as a promising technique for analyzing low molecular weight compounds has gained increasing attention. Several important review articles on SALDI‐TOF MS for small molecules have been published over the past few years . However, the above reviews mostly focused on positive ion SALDI‐TOF MS for the analysis of small molecules, which commonly leads to unspecific cationization during LDI process.…”