Fusarium solani causing stem rot and wilt of lucky Bamboo (Dracaena sanderiana) in India-first record

Kumar, Narendra; Dubey, S. C.; Kumar, Pardeep; Khurana, S. M. P.

doi:10.1007/s42360-019-00119-8

Cited by 4 publications

(7 citation statements)

References 5 publications

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“…(see Figure ) is one of the major challenges in tissue culture propagation of the ornamental plant D. sanderiana . [ 27 ] We obtained healthy plant nodes free from microbial contaminations by sterilizing nodes using nanohybrids as the sterilizing agent for 1 month period (see Figure S11, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

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Visible Light Active Silver Decorated Iron Titanate/Titanium Dioxide Nanohybrid for Sterilization of Explants Grown by In Vitro Technique

Seneviratne

Munaweera

Peiris

et al. 2022

Adv Materials Technologies

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Visible light (VL) active silver decorated iron titanate/titanium dioxide (Ag‐FeTiO3/TiO2) nanohybrids derived from natural ilmenite sand effectively cleanse the exophytic plant pathogens in explants grown by in vitro techniques. VL active nanohybrid is synthesized by acid‐hydrolysis of natural ilmenite, followed by precipitation of the lixivium and decoration with Ag onto FeTiO3/TiO2 nanoparticles, and finally calcination under a nitrogen environment to obtain Ag‐FeTiO3/TiO2. A morphological study conducted using transmission electron microscopy (TEM) confirms the formation of Ag‐FeTiO3/TiO2. Powder X‐ray diffraction (PXRD) analysis shows that the nanohybrid primarily consists of anatase, iron titanate (FeTiO3), Ag2O, and Ag. X‐ray photoelectron spectroscopy (XPS) study suggests the presence of Ag0/Ag2O/Fe2O3 composites on the FeTiO3/TiO2 particle surface. The optical band gap significantly changes from 3.14 eV (ilmenite) to 2.80 eV after the decoration of FeTiO3/TiO2 with Ag. This nanohybrid is utilized as a surface‐sterilizing agent for in vitro establishment of the Dracaena sanderiana Sander ex Mast plant. Findings illustrate Ag‐FeTiO3/TiO2 nanohybrid‐based photo‐sterilization leads to the survival of 90% of the microbes’ free cultures while the material can be re‐used due to its photocatalytic behavior. Thus, the newly synthesized nanohybrid can replace harmful sterilization agents used in tissue culture techniques.

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Section: Resultsmentioning

confidence: 99%

“…Ag‐FeTiO 3 /TiO 2 nanohybrid was derived from Sri Lankan ilmenite (beach sand) and utilized in the VL region to eliminate plant exophytic pathogens. [ 27 ] Herein, we used commercially grown Dracaena sanderiana Sander (“White”) ex Mast. as the explant for the tissue culture experiment.…”

Section: Introductionmentioning

confidence: 99%

Visible Light Active Silver Decorated Iron Titanate/Titanium Dioxide Nanohybrid for Sterilization of Explants Grown by In Vitro Technique

Seneviratne

Munaweera

Peiris

et al. 2022

Adv Materials Technologies

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“…Universal ITS 5 and ITS 4 primers were used to amplify the ITS region (White et al, 1990). PCR amplification was carried out using the protocol described by Kumar et al (2019). For analysis, the amplified product (15 μL) was electrophoresed in an agarose gel (1.2%) containing 1x TBE buffer for 80 min at a current of 80 Volt.…”

Section: Methodsmentioning

confidence: 99%

“…Universal ITS 5 and ITS 4 primers were used to amplify the ITS region (White et al, 1990). PCR amplification was carried out using the protocol described by Kumar et al (2019).…”

Section: Conserved Gene Sequence Amplification and Sequencingmentioning

confidence: 99%

First report of Diaporthe helianthi infecting safflower: A threat to its cultivation in India

Akhtar

Kumar

Kiran

et al. 2023

Journal of Phytopathology

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During seed health testing of 160 accessions of safflower, we observed some fungal growth on seed surface of four accessions i.e., IC337954, IC338197, EC182199 and EC181301 which were received from Hyderabad, Telangana, India for long‐term conservation in the National Genebank, ICAR‐National Bureau of Plant Genetic Resources, New Delhi, India. The associated fungus was morphologically identified as Diaporthe helianthi. Later on, the identity was confirmed as Diaporthe helianthi (Desm.) Grove by the sequencing of internal transcribed spacer (ITS) region (GenBank Id: KM979906.1). During seed health testing, seed infection of 30.0 to 70.0 per cent was recorded with no seed germination in infected seeds. Previous findings revealed that D. helianthi is host‐specific pathogen causing stem canker to sunflower only and the fungus is seed‐borne. As a destructive pathogen causing substantial yield losses, especially to sunflower, it may be a serious threat to safflower cultivation in the country and anywhere else. Therefore, the detection and identification of D. helianthi can be helpful in alarming the threat at an early stage thereby minimizing the probable damage to safflower cultivation in near future.

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“…Initial symptoms included a few yellowing rotted areas on the roots, a visible brown discoloration, and rotted areas on the base of the stems as the cortical tissues showed a distinct brown discoloration of water-soaked spots. These lesions became longer up and down and had-dark brown centers with a brown margin, and eventually severe wilting resulted in a significant loss of lucky bamboo plants 5 , 6 .…”

Section: Introductionmentioning

confidence: 99%

Characterization and control of Rhizoctonia solani affecting lucky bamboo (Dracaena sanderiana hort. ex. Mast.) using some bioagents

Abdel-Rahman

Abdel-Megeed

Salem

2023

Sci Rep

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In a survey conducted during the period of March–May 2019 in nurseries, warehouses, and shops at three governorates (Alexandria, El-Behera, and Giza governorates, Egypt), symptoms of root rot, basal stem rot, and wilt disease complex were observed in the lucky bamboo (Dracaena sanderiana hort. ex. Mast.). The highest disease infection percentage was found in lucky bamboo collected from Alexandria City (47.67%), while the highest disease severity was in lucky bamboo collected from El-Behera Governorate (35.19%). Rhizoctonia solani, Fusarium oxysporum, F. solani, Aspergillus niger, and Alternaria alternate were isolated and identified in the infected lucky bamboo samples. R. solani isolates were the most dominant among the recovered fungal species with a percentage of 80.89% of the total isolates (246). Pathogenicity tests showed that R. solani was the most pathogen with 100% disease infection and 76.67% disease severity. Molecular identification characterized R. solani isolate as R. solani AUMC 15120, MZ723906. Meanwhile, four biological control agents (bioagents) were isolated from the healthy lucky bamboo samples and identified based on cultural, morphological, microscopic characteristics, and the molecular phylogenetic analysis as Clonostachys rosea AUMC 15121, OL461708; Bacillus circulans TAG1, MW441316; B. siamensis TAP1, MW441318 and Ochrobactrum anthropi TAM1, MW441317. The four bioagents showed potential inhibition of R. solani in vitro as well as in vivo on lucky bamboo plants in vase treatments compared to the untreated inoculated control as well as certain fungicides and biocides used (Moncut, Rizolex-T, Topsin-M, Bio-Zeid, and Bio-Arc). The bioagent O. anthropi showed the highest inhibition growth (85.11%) of the in vitro R. solani colony, which was not significantly different from the biocide Bio-Arc (83.78%). However, C. rosea, B. siamensis and B. circulans showed inhibition values of 65.33, 64.44, and 60.44%, respectively. On the other hand, the biocide Bio-Zeid showed less inhibitory effect (43.11%), while the lowest growth inhibition was recorded by Rizolex-T (34.22%) and Topsin-M (28.67%). Furthermore, the in vivo experiment supported the in vitro results for the most effective treatments, where all the treatments significantly decreased the percentage of infection and disease severity compared to the inoculated untreated control. Additionally, the bioagent O. anthropi showed the highest effect, i.e., the lowest disease incidence and disease severity being 13.33% and 10%, compared to 100% and 75%, respectively, in the untreated inoculated control. This was not significantly different from the fungicide Moncut (13.33% and 21%) and from the bioagent C. rosea (20% and 15%) treatments for both parameters, respectively. In conclusion, the bioagents O. anthropi MW441317 at 1 × 108 CFU/ml as well as C. rosea AUMC15121 at 1 × 107/ml proved to be efficient to control R. solani causing root rot, and basal stem rot on lucky bamboo, compared to fungicide Moncut and can be used for disease management without the negative impact of the chemical control. Furthermore, this is the first report of the isolation and identification of Rhizoctonia solani, a pathogenic fungus, and four biocontrol agents (Bacillus circulans, B. siamensis, Ochrobactrum anthropi and Clonostachys rosea) associated with the healthy lucky bamboo plants.

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Fusarium solani causing stem rot and wilt of lucky Bamboo (Dracaena sanderiana) in India-first record

Cited by 4 publications

References 5 publications

Visible Light Active Silver Decorated Iron Titanate/Titanium Dioxide Nanohybrid for Sterilization of Explants Grown by In Vitro Technique

Visible Light Active Silver Decorated Iron Titanate/Titanium Dioxide Nanohybrid for Sterilization of Explants Grown by In Vitro Technique

First report of Diaporthe helianthi infecting safflower: A threat to its cultivation in India

Characterization and control of Rhizoctonia solani affecting lucky bamboo (Dracaena sanderiana hort. ex. Mast.) using some bioagents

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