Fusarium oxysporum homogenates and jasmonate induce limited sanguinarine accumulation in Argemone mexicana cell cultures

Abstract

In vitro cultures of Argemone mexicana (Papaveraceae) were induced from leaves of mature plants. Sanguinarine, a benzophenanthridine, was the main alkaloid in the cultures, even in the absence of inducers of secondary metabolism. The accumulation of this metabolite was increased by adding methyl jasmonate and fungal elicitors, although in a limited fashion in comparison to other sanguinarine-producing species. Evidence of a transport mechanism, which may be related to the magnitude of the response, was obtained based on the fluorescent properties of bezophenathridines in the elicited cultures.

Yeast extract induction of sanguinarine biosynthesis is partially dependent on the octadecanoic acid pathway in cell cultures of Argemone mexicana L., the Mexican poppy

“…Over the years, a number of in vitro cell lines derived from different tissues have been generated in our laboratory. In all cases, the ability to produce berberine was lost in the undifferentiated cultures, but they were able to accumulate sanguinarine and DHSA (Trujillo-Villanueva et al 2010Vázquez-Flota et al 2012). One of these cell lines, named AmMiF, accumulates significant amounts of sanguinarine without any induction treatment .…”

The sequential exposure to jasmonate, salicylic acid and yeast extract promotes sanguinarine accumulation in Argemone mexicana cell cultures

“…Due to sanguinarine toxicity, cells require to control its internal amounts (Trujillo-Villanueva et al 2010). The development of discrete auto fluorescent foci and their distribution in the sequentially induced cultures, suggest the compartmentalization of sanguinarine in vesicles, probably derived from the endoplasmic reticulum, which later on could be dispatched to the exterior, as part of mechanism to avoid its deleterious effects (Fig.…”

“…Discrete fluorescent spots were also observed scattered in the cytoplasm, between the nucleus and the cell periphery. These fluorescent areas might correspond to membranous vesicles, and could be a part of a mechanism for the isolation and excretion of alkaloids that the cell uses to cope with the toxic effects of sanguinarine (Alcantara et al 2005;Sirikantaramas et al 2008) since they were not noticed observed prior to exposure to YE (Trujillo-Villanueva et al 2010). The operation of such excretion mechanism may account for some sanguinarine recovered from the spent medium (over 10% of the total sanguinarine produced).…”

“…Besides its antimicrobial effects, sanguinarine also presents cytotoxic and antiviral activities (Capasso et al 2002). We have developed an A. mexicana cell culture that accumulates sanguinarine, but not berberine (Trujillo-Villanueva et al 2010). In these cultures, the individual exposure to fungal homogenates, methyl jasmonate (MeJa) or salicylic acid (SA) resulted in an increase in sanguinarine accumulation, but in a limited fashion when compared to other species (Trujillo-Villanueva et al 2010).…”

“…We have developed an A. mexicana cell culture that accumulates sanguinarine, but not berberine (Trujillo-Villanueva et al 2010). In these cultures, the individual exposure to fungal homogenates, methyl jasmonate (MeJa) or salicylic acid (SA) resulted in an increase in sanguinarine accumulation, but in a limited fashion when compared to other species (Trujillo-Villanueva et al 2010). Here, we report that the sequential exposure to three different elicitors: MeJa, SA and yeast extract (YE), with 24 h between them, promoted increased sanguinarine production which was superior to the sole exposure to each one as well as to simultaneous exposure.…”