Fusarium oxysporum f. sp. phaseoli genetic variability assessed by new developed microsatellites

Abstract

Fusarium oxysporum f. sp. phaseoli (Fop) J.B. Kendrich & W.C. Snyder is the causal agent of Fusarium wilt of common bean (Phaseolus vulgaris L.). The objective of this study was to develop microsatellite markers (SSRs) to characterize the genetic diversity of Fop. Two libraries enriched with SSRs were developed and a total of 40 pairs of SSRs were characterized. Out of these, 15 SSRs were polymorphic for 42 Fop isolates. The number of alleles varied from two to ten, with an average of four alleles per locus and an average PIC (Polymorphic Information Content) of 0.38. The genetic diversity assessed by microsatellites for Fop was low, as expected for an asexual fungus, and not associated with geographic origin, but they were able to detect enough genetic variability among isolates in order to differentiate them. Microsatellites are a robust tool widely used for genetic fingerprinting and population structure analyses. SSRs for Fop may be an efficient tool for a better understanding of the ecology, epidemiology and evolution of this pathogen.

Assessment of resistance in common bean to Fusarium oxysporum f. sp. phaseoli using different inoculation and evaluation methods

“…isolates, the race as well as the diversity of physiological races. This is important for the characterization of resistant bean genotypes to the fungus (Karimian et al 2010, Henrique et al 2015, Cruz et al 2018, Wamalwa et al 2018, Petkar et al 2019, Sasseron et al 2020.…”

Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean

“…Fop is genetically variable and often found in common bean growing in different countries and regions; up to now, seven pathogenic races related to geographical regions are cited in the literature [ 20 , 80 , 81 ], and new races like UFV01 and IAC18001 occur, supporting Fop pathogenic evolution [ 82 ]. However, mutations and recombination between avirulence genes (avr) in sexually reproducing pathogens are postulated as the mechanisms responsible for variation in races [ 83 ].…”

Historical Differentiation and Recent Hybridization in Natural Populations of the Nematode-Trapping Fungus Arthrobotrys oligospora in China

“…STRs (or microsatellites) have been extensively used for molecular ecology and population genetic studies due to their high-level polymorphisms and high reproducibility in eukaryotic genomes [ 32 , 33 , 34 , 35 , 36 ]. In this study, the STR markers were developed based on the genome sequence of A. oligospora strain ATCC24927 (GenBank accession ADOT00000000), using the MISA software (Available online: (accessed on 12 April 2021)).…”