Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean

Abstract

Fusarium wilt (Fusarium oxysporum f. sp. phaseoli, Fop) is one of the main fungal soil diseases in common bean. The aim of the present study was to identify genomic regions associated with Fop resistance through genome-wide association studies (GWAS) in a Mesoamerican Diversity Panel (MDP) and to identify potential common bean sources of Fop’s resistance. The MDP was genotyped with BARCBean6K_3BeadChip and evaluated for Fop resistance with two different monosporic strains using the root-dip method. Disease severity rating (DSR) and the area under the disease progress curve (AUDPC), at 21 days after inoculation (DAI), were used for GWAS performed with FarmCPU model. The p-value of each SNP was determined by resampling method and Bonferroni test. For UFV01 strain, two significant single nucleotide polymorphisms (SNPs) were mapped on the Pv05 and Pv11 for AUDPC, and the same SNP (ss715648096) on Pv11 was associated with AUDPC and DSR. Another SNP, mapped on Pv03, showed significance for DSR. Regarding IAC18001 strain, significant SNPs on Pv03, Pv04, Pv05, Pv07 and on Pv01, Pv05, and Pv10 were observed. Putative candidate genes related to nucleotide-binding sites and carboxy-terminal leucine-rich repeats were identified. The markers may be important future tools for genomic selection to Fop disease resistance in beans.

Genetic variation in a tepary bean (Phaseolus acutifolius A. Gray) diversity panel reveals loci associated with biotic stress resistance

“…The minor allele of SNP S04_50664971 had the largest effect size of any Fusarium‐associated SNPs, decreasing the Fusarium severity rating by 1.5, followed by the minor allele for SNP S02_6425026 that reduced Fusarium ratings by 1.3. A previous GWAS study in common bean that examined resistance to two strains of Fusarium wilt yielded QTL on several chromosomes including a cluster of NLR genes on chromosome Pv11 (Paulino et al., 2021) yet the QTL associated with Fusarium wilt resistance in tepary bean was not syntenic with the common bean chromosome Pv11 QTL.…”

Genetic architecture of inducible and constitutive metabolic profile related to drought resistance in qingke (Tibetan hulless barley)

“…Wilting is the external manifestation of water potential in plants. It has been proved that leaf wilting is closely related to the plant drought resistance and can be an indicator for the evaluation of plant drought resistance ( Alidu et al, 2019 ; Ye et al., 2020 ), scores based on the leaf wilting were also used for genome-wide association analysis in biotic ( Paulino et al., 2021 ) or abiotic ( Ahmed et al., 2021 ) stress research. To assess the drought resistance of highland barley directly and accurately, the wilting rate of qingkes under drought stress was counted and divided into 0 to 4 grades.…”

Inheritance of resistance to Fusarium wilt in the carioca grain type common bean

“…Isolate FOP UFV 01, which was used in this assay, was collected in Coimbra (Minas Gerais, Brazil) from plants of the black type grain cultivar Meia Noite, which exhibited the typical symptoms of Fusarium wilt (Pereira et al 2013). This isolate was chosen because it was previously identified using molecular markers (Cruz et al 2018) and has been widely characterized and applied in common bean breeding programs owing to its aggressiveness (Batista et al 2016, Batista et al 2017, Cruz et al 2018, Sasseron et al 2020, Paulino et al 2021.…”