Fusarium oxysporum f. sp. lycopersici C2H2 transcription factor FolCzf1 is required for conidiation, fusaric acid production, and early host infection

Yun, Yingzi; Zhou, Xin; Yang, Shuai; Wen, Ya; You, Haixia; Zheng, Yongqiang; Norvienyeku, Justice; Shim, Won‐Bo; Wang, Zonghua

doi:10.1007/s00294-019-00931-9

Cited by 25 publications

(24 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the C2H2 zinc finger domain, the presence of a zinc atom with two conserved cysteine residues at one end of the β‐sheet and two conserved histidine residues at the α‐helix C‐terminus ensure the stability of the zinc finger structure (Wolfe et al, 2000). In F. oxysporum , a total of five C2H2 ZFs have been identified and functionally tested, namely, PacC, ZafA, Ste12, FolCzf1, and Con7‐1 (Table 2) (Asuncion Garcia‐Sanchez et al, 2010; Caracuel et al, 2003; López‐Berges, 2020; Ruiz‐Roldan et al, 2015; Yun et al, 2019). GATA proteins, a family of GATA‐binding zinc finger transcription factors, play diverse functions in fungi and consist of one or two zinc‐binding modules with four cysteines embedded in the sequence Cys‐X2‐Cys‐X17/18‐Cys‐X2‐Cys and an adjacent basic region (Teakle & Gilmartin, 1998).…”

Section: Major Families Of Tfs In F Oxysporummentioning

confidence: 99%

See 1 more Smart Citation

Current progress on pathogenicity‐related transcription factors in Fusarium oxysporum

Zuriegat

Zheng

Liu

et al. 2021

Molecular Plant Pathology

Self Cite

View full text Add to dashboard Cite

Fusarium oxysporum is a well‐known soilborne plant pathogen that causes severe vascular wilt in economically important crops worldwide. During the infection process, F. oxysporum not only secretes various virulence factors, such as cell wall‐degrading enzymes (CWDEs), effectors, and mycotoxins, that potentially play important roles in fungal pathogenicity but it must also respond to extrinsic abiotic stresses from the environment and the host. Over 700 transcription factors (TFs) have been predicted in the genome of F. oxysporum, but only 26 TFs have been functionally characterized in various formae speciales of F. oxysporum. Among these TFs, a total of 23 belonging to 10 families are required for pathogenesis through various mechanisms and pathways, and the zinc finger TF family is the largest family among these 10 families, which consists of 15 TFs that have been functionally characterized in F. oxysporum. In this review, we report current research progress on the 26 functionally analysed TFs in F. oxysporum and sort them into four groups based on their roles in F. oxysporum pathogenicity. Furthermore, we summarize and compare the biofunctions, involved pathways, putative targets, and homologs of these TFs and analyse the relationships among them. This review provides a systematic analysis of the regulation of virulence‐related genes and facilitates further mechanistic analysis of TFs important in F. oxysporum virulence.

show abstract

Section: Major Families Of Tfs In F Oxysporummentioning

confidence: 99%

“…The C2H2 TF FolCzf1 plays an important role in early infection in F. oxysporum (Yun et al, 2019). Deletion of FolCZF1 resulted in a loss of virulence and decreased fusaric acid production (Yun et al, 2019).…”

Section: Transcriptional Regulation Of Direct Virulence Factors In F Oxysporum Including Effectors Cwdes and Mycotoxinsmentioning

confidence: 99%

Current progress on pathogenicity‐related transcription factors in Fusarium oxysporum

Zuriegat

Zheng

Liu

et al. 2021

Molecular Plant Pathology

Self Cite

View full text Add to dashboard Cite

show abstract

“…C 2 H 2 ZFPs comprise of an abundant nucleic acid-binding protein family in the genomes of eukaryotes (Schumacher et al, 2004). C 2 H 2 ZFPs display a variety of functions, which not only participate in transcriptional regulation of target genes but also act in other cellular functions that are probably associated with the zinc-finger domain ( Englbrecht et al, 2004 ; Foster et al, 2003 ; Yun et al, 2019 ). In this study, we found that the calcineurin-responsive TF CgCrzA played important roles in vegetative growth, asexual reproduction, pathogenesis, and also CWI.…”

Section: Discussionmentioning

confidence: 99%

“…In eukaryotic organisms, transcription factors (TFs) regulate a wide range of physiological processes by binding to specific DNA sites, where activation or repression of gene transcription occurs ( Kusuya et al, 2017 ; Pabo and Sauer, 1992 ; Yun et al, 2019 ). Based on the differences of DNA-binding domain types, TFs can be divided into different classes, including basic region leucine zipper (bZIP), MADS-box, myb, helix-loop-helix, homeobox, and zinc fingers ( Chen et al, 2002 ; Pabo and Sauer, 1992 ).…”

mentioning

confidence: 99%

Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides

Wang

Pan

et al. 2020

Plant Pathol J

View full text Add to dashboard Cite

“…FolCZF1 that encodes a CZF TF in F. oxysporum f. sp. lycopersici is essential for conidiation, infection and fusaric acid production (Yun et al 2019).…”

Section: Introductionmentioning

confidence: 99%

FpCzf14 is a putative C2H2 transcription factor regulating conidiation in Fusarium pseudograminearum

et al. 2020

View full text Add to dashboard Cite

C2H2 zinc finger transcription factors such as FlbC and Msn2, have broad regulatory roles in fungal growth and conidiation. In the present study, we cloned and characterized a C2H2 zinc finger transcription factor gene, FpCzf14, in the wheat pathogen Fusarium pseudograminearum. FpCzf14 was localized to the nuclei. The expression of FpCzf14 was significantly upregulated in conidia, suggesting that FpCzf14 might contribute to conidiation. Further analysis of the fpczf14-deleted mutant (Δfpczf14) demonstrated that it exhibited defect in conidiation, and this defect was restored in the complemented strain Δfpczf14-C expressing FpCzf14, demonstrating that FpCzf14 was essential for conidiation. Moreover, FpCzf14 was required for mycelial growth and pathogenicity of F. pseudograminearum. Microscopic observation results showed that Δfpczf14 produced only very few penetration pegs and invasive hyphae inside host tissues compared with WT and Δfpczf14-C. Additionally, results of reverse transcription quantitative PCR (RT-qPCR) showed that FpCzf14 regulated expression of several conidiation-related genes in F. pseudograminearum. In conclusion, FpCzf14, as a core regulatory gene in conidiation, provides new insights into the mechanism of conidiation in F. pseudograminearum.

show abstract

Fusarium oxysporum f. sp. lycopersici C2H2 transcription factor FolCzf1 is required for conidiation, fusaric acid production, and early host infection

Cited by 25 publications

References 59 publications

Current progress on pathogenicity‐related transcription factors in Fusarium oxysporum

Current progress on pathogenicity‐related transcription factors in Fusarium oxysporum

Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides

FpCzf14 is a putative C2H2 transcription factor regulating conidiation in Fusarium pseudograminearum

Contact Info

Product

Resources

About