Fusarium kyushuense sp. nov. from Japan

Aoki, Takayuki; O’Donnell, Kerry

doi:10.1007/bf02461571

Cited by 42 publications

(18 citation statements)

References 10 publications

(15 reference statements)

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“…Never any indication for a contamination of the culture with A. parasiticus, even after repeated checking for purity, could be observed. According to Aoki and O'Donnell (1998), F. kyushuense NRRL 3509 is a degenerated strain with the capacity to sector formation and variable pigmentation. This could also be observed in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…This strain was originally deposited as F. tricinctum NRRL 3509 in the culture collection of the MRI, but it is now reclassified as F. kyushuense NRRL 3509 (Aoki and O'Donnell 1998). The original type culture of F. kyushuense NRRL 3509 was gratefully obtained from the National Institute of Agrobiological Sciences, Kannondai, Tsukuba, Ibaraki 305-8602, Japan (www.gene.affrc.go.jp).…”

Section: Rationale Of the Analysismentioning

confidence: 99%

“…The strain was later classified as F. sporotrichioides var. tricinctum (Joffe and Palti 1975) and was reclassified as F. kyushuense by Aoki and O'Donnell (1998). Both clones of this strain were used for all subsequent analytical experiments.…”

Section: Rationale Of the Analysismentioning

confidence: 99%

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A strain of Fusarium kyushuense is able to produce aflatoxin B1 and G1

et al. 2009

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A strain of F. kyushuense is able to produce aflatoxin B1 and G1 under certain growth conditions. This was ultimately demonstrated by LC-MS analysis. By using a microarray covering oligonucleotides specific for the aflatoxin pathway genes of A. flavus, the expression of homologues of nearly the complete aflatoxin biosynthesis gene cluster was detected in F. kyushuense under producing conditions, e.g., after growth in liquid YES medium. In contrast, after growth of F. kyushuense on solid YES medium, only a very low transcription level of one of the homologues (nor-1) could be observed by microarray and Real Time PCR demonstrating differential gene expression. The cross-hybridization of the F. kyushuense mRNA to the A. flavus aflatoxin biosynthesis gene oligonucleotides of the array indicates homology between the sequences. PCR analyses with primers deduced from the nor-1 gene sequence of the aflatoxin biosynthesis gene cluster from A. flavus revealed the presence of a homologue of this gene in F. kyushuense. Sequencing of PCR fragments of the nor-1 homologue revealed a sequence similarity of >90% to the nor-1 gene of A. flavus.

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Section: Discussionmentioning

confidence: 99%

Section: Rationale Of the Analysismentioning

confidence: 99%

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A strain of Fusarium kyushuense is able to produce aflatoxin B1 and G1

et al. 2009

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“…However, reports of a new species, Fusarium kyushuense, first described in Japan (Aoki and O'Donnell, 1998) but recently reported causing Fusarium wilt of tobacco in China (Wang et al, 2013) are of concern, as it is unknown whether current resistance genes in tobacco are effective against this pathogen. The development and use of wilt-resistant tobacco cultivars must continue indefinitely.…”

Section: Rotationmentioning

confidence: 97%

Fusarium wilt of tobacco

LaMondia

2015

Crop Protection

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“…Recently, the use of molecular methods based on sequence analysis of multiple genes has contributed to a more accurate identification of Fusarium and has showed great inter -and intra-species diversity, that in some cases represent complexes of species (16,17). Among the most important molecular markers used to identify Fusarium are the 5' region of the 28s rdnA subunit (18) and the gene encoding the elongation factor of translation 1α (tef1-α), considered latter as the most informative gene for identification to species or species complex of the genus (19).…”

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confidence: 99%

Molecular identification of clinical isolates of Fusarium in Colombia

et al. 2018

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Objective Identifying Fusarium isolates from mycosis symptomatic patients through molecular techniques as PCR and sequencing. Methods In this study, samples were taken from 101 mycosis symptomatic patients in-between [2004][2005][2006]. To determine isolates belonging to the Fusarium genus, the DNAr 28S region was amplified through PCR and specific PCR primers further confirmed their identity to the species level. Additionally, in order to confirm the identity of the species of the isolates, 75 isolates of these were analyzed by partial sequencing of the 28S rDNA and the TEF1-α gene. Results The 28S rDNA portion detected all 101 isolates as belonging to Fusarium and the PCR specific primers detected 52 and 29 isolates as F. oxysporum and F. solani, respectively; 34 and 41 of these, afterwards studied by partial sequencing of the 28S rDNA and TEF1-α genes respectively, were effectively identified by the technique. Conclusion From all the molecular markers used to identify Fusarium isolates, the sequence of the TEF1-α gene provided the best resolution in the identification of species level; however it is possible to discriminate between F. oxysporum and F. solani isolates by PCR, in most of the cases, what is important considering the simplicity of the technique and a faster diagnosis.Key Words: PCR; rDNA; sequence analysis; elongation factor (source: MeSH, NLM). RESUMENObjetivo Identificar aislamientos de Fusarium en pacientes con micosis por medio de las técnicas moleculares de PCR y secuenciación. Métodos Se tomaron 101 muestras de pacientes con micosis sintomática, entre los años 2004 y 2006. Para la detección de aislamientos como pertenecientes al género Fusarium, se amplificó parcialmente por PCR la región 28S del DNAr; y posteriormente -para la detección de la especie de Fusarium-se utilizaron cebadores específicos para F. oxysporum y F. solani. La verificación de la identidad de la especie de los aislamientos se hizo por secuenciación parcial de los genes 28S DNAr y TEF1-α. Resultados El total de 101 aislamientos fueron detectados como pertenecientes al género Fusarium utilizando un cebador universal de la region 28S DNAr; 52 y 29 aislamientos se detectaron como F. oxysporum o F. salani, respectivamente con los cebadores específicos, y la secuenciación parcial de los genes 28S rDNA o TEF1-α confirmó la identidad de las especies. Conclusión La secuenciación parcial del gen TEF1-α es aún el mejor marcador molecular para identificar aislamientos de Fusarium a nivel de especie. Sin embargo, en la mayoría de los casos es posible discriminar entre aislamientos de F. oxysporum y F. solani por PCR con cebadores específicos, lo que proporciona una ventaja importante considerando la simplicidad de la técnica y el rápido diagnóstico.Palabras Clave: ADN ribosómico; PCR; secuenciación; elongación (fuente: DeCS, BIREME).

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Fusarium kyushuense sp. nov. from Japan

Cited by 42 publications

References 10 publications

A strain of Fusarium kyushuense is able to produce aflatoxin B1 and G1

A strain of Fusarium kyushuense is able to produce aflatoxin B1 and G1

Fusarium wilt of tobacco

Molecular identification of clinical isolates of Fusarium in Colombia

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