Fusarium culmorum is a single phylogenetic species based on multilocus sequence analysis

Obanor, Friday; Erginbaş-Orakci, Gül; Tunalı, Berna; Nıcol, J. M.; Chakraborty, Sukumar

doi:10.1016/j.funbio.2010.07.001

Cited by 46 publications

(52 citation statements)

References 57 publications

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“…A comparison with previously available EF-1a sequences was performed by downloading all available reference sequences from the nucleotide database in NCBI using keywords 'Fusarium culmorum' and 'elongation factor' followed by a manual check for the correct sequence correspondence (Table S1). The FHB-derived F. culmorum data set from Beyer et al (2014) as well as the data set of Obanor et al (2010), which both provide information on the disease type, were included. The SNP designation was counted for all sequences, comparing the presence of T or A, to distinguish two types of isolates.…”

Section: Strain Characterization and Storagementioning

confidence: 99%

“…Screening publicly available data sets on F. culmorum EF-1a sequences (Table S1) revealed that the A-haplotype form was present in some isolates collected in Tunisia, Syria, Iran (Obanor et al, 2010), Turkey (O'Donnell et al, 2012), Australia (Ward et al, 2008) and Norway (Kristensen et al, 2005) and thus seems to occur worldwide (Fig. S1).…”

Section: Ef-1a Diversity and Disease Associationmentioning

confidence: 99%

“…S1). No further statistical analysis could be performed to validate the hypothesis of association with FRR due to the very limited information on sample sources: disease type was only described for isolates originating from Tunisia, Iran and Syria, (Obanor et al, 2010), but the small number of isolates did not permit statistical processing with satisfactory power (Table S1). …”

Section: Ef-1a Diversity and Disease Associationmentioning

confidence: 99%

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Fusarium species and chemotypes associated with fusarium head blight and fusarium root rot on wheat in Sardinia

et al. 2015

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Environmental conditions in Sardinia (Tyrrhenian Islands) are conducive to fusarium root rot (FRR) and fusarium head blight (FHB). A monitoring survey on wheat was carried out from 2001 to 2013, investigating relations among these diseases and their causal agents. FHB was more frequently encountered in the most recent years while FRR was constantly present throughout the monitored period. By assessing the population composition of the causal agents as well as their genetic chemotypes and EF-1a polymorphisms, the study examined whether the two diseases could be differentially associated to a species or a population. Fusarium culmorum chemotypes caused both diseases and were detected at different abundances (88% 3-ADON, 12% NIV). Fusarium graminearum (15-ADON genetic chemotype) appeared only recently (2013) and in few areas as the causal agent of FHB. In F. culmorum, two haplotypes were identified based on an SNP mutation located 34 bp after the first exon of the EF-1a partial sequence (60% adenine, 40% thymine); the two populations did not segregate with the chemotype but the A-haplotype was significantly associated with FRR in the Sardinian data set (P = 0Á001), suggesting a possible fitness advantage of the A-haplotype in the establishment of FRR that was neither dependent on the sampling location nor the sampling year. The SNP determining the Sardinian haplotype is distributed worldwide. The question whether the A-haplotype segregates with characters facilitating FRR establishment will require further validation on a specifically sampled international data set.

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Section: Strain Characterization and Storagementioning

confidence: 99%

Section: Ef-1a Diversity and Disease Associationmentioning

confidence: 99%

Section: Ef-1a Diversity and Disease Associationmentioning

confidence: 99%

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Fusarium species and chemotypes associated with fusarium head blight and fusarium root rot on wheat in Sardinia

et al. 2015

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“…The diseases are caused by more than ten Fusarium species. Fusarium culmorum, a single phylogenetic species, is a major causal agent of HB in many agro-ecological regions located in West-Asia, Europe and America (Obanor et al, 2010;Pasquali et al, 2016). Diseases caused by F. culmorum resulted in severe economic losses due to decrease in crop quality and quantity (Smiley et al, 2005;Miedaner et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum

Zümrüt

Tunalı

Kansu

et al. 2018

Turkish JAF Sci.Tech.

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Fusarium culmorum is predominating causal agent of head blight (HB) and root rot (RR) in cereals worldwide. Since F. culmorum has a great level of genetic diversity and the parasexual stage is assumed for this phytopathogen, characterization of isolates from different regions is significant step in food safety and controlling the HB. In this study, it was aimed to characterize totally 37 F. culmorum isolates from Turkey via sequence related amplified polymorphism (SRAP) marker based genotyping. MAT-1/MAT-2 type assay was also used in order to reveal intraspecific variation in F. culmorum. MAT-1 and MAT-2 specific primer pairs for mating assays resulted in 210 and 260 bp bands, respectively. 11 of isolates were belonged to MAT-1 type whereas 19 samples were of MAT-2. Remaining 7 samples yielded both amplicons. Totally 9 SRAP primer sets yielded amplicons from all isolates. Genetic similarity values were ranged from 39 to 94.7%. Total band number was 127 and PCR product sizes were in the range of 0.1-2.5 kb. Amplicon numbers for individuals were ranged from 1 to 16. According to data obtained from current study, SRAP based genotyping is powerful tool for supporting the data obtained from investigations including phenotypic and agro-ecological characteristics. Findings showed that SRAP-based markers could be useful in F. culmorum characterization.

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“…Later PHO gene was used in four-locus phylogeny analysis [33] which allows to distinguish a number of isolates from Siberia and the Far East, originally identified as F. poae, as a separate species F. sibiricum. At the same time PHO gene is polymorphic enough to clearly distinguish F. culmorum from the closely related F. cerealis [41]. We used PHO as a marker for intra-and interspecific polymorphism study in F. avenaceum, F. tricinctum, F. acuminatum, F. torulosum, producing toxins of enniatin group and moniliformin [32].…”

mentioning

confidence: 99%

MOLECULAR GENETIC APPROACHES FOR INVESTIGATION OF TAXONOMY AND SPECIFIC IDENTIFICATION OF TOXIN-PRODUCING Fusarium SPECIES: ACHIEVEMENTS AND PROBLEMS (review)

Stakheev¹,

Samokhvalova²,

Ryazantsev³

et al. 2016

S-h. biol.

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A b s t r a c tAscomycetous fungi of the genus Fusarium, which was separated as a single taxonomic group in 1809 by Heinrich Friedrich Link, are distributed worldwide and have different ecological niches. They have been found in soil and plants as endophytes, saprotrophs and parasites. Infections of agricultural plants, caused by Fusarium fungi, lead to annual damage, estimated in hundred million dollars. In addition to decrease harvest quality and quantity, Fusarium fungi are able to produce toxic metabolites (fusariotoxins). The consumption of fusariotoxin-contaminated winter grain led to deaths of tens of thousands of people in Volga and Ural regions in 1930s-1940s. These factors make necessary investigation of morphology, biochemistry and genetics of those organisms. Nowadays there is no universal taxonomic system of the genus Fusarium, so it is not possible to identify an isolate to species level in many cases. The high variability of morphological structures, and, on the other side, their similarity for closely related species, are major problems for researchers which use classical methods for identification and systematization of this group of fungi. Today, the molecular methods, based on the use of specific DNA sequence, are playing an increasingly important role in Fusarium systematics. The application of this approach has led to establishment of a number of new species in the genus. T. Yli-Mattila et al. (2009, 2011 used multilocus phylogenetic analysis to describe the novel species F. sibiricum and F. ussurianum which were found in Siberia and Far East and proved to be morphologically similar to F. graminearum and F. langsethiae, respectively. The authors of this paper identified the species F. torulosum (morphologically similar to F. avenaceum) in Russia for the first time based on the use of DNA markers. It is no less important that the analysis of interand intraspecific polymorphism makes possible the development of highly-specific assays for molecular detection of Fusarium fungi, including ones which infect plants and produce mycotoxins. The use of these assays (for example, ones described by M. Nicolaisen et al., 2009 and A.A. Stakheev et al., 2011) allows not only to detect the contamination of plants by mycotoxin producers, but also to estimate the quantity of their content in a probe. At the same time, a lot of questions are still unanswered: particularly, simultaneous application of different species concepts (morphological, biological and phylogenetic) makes it difficult to develop universal taxonomic system of the genus; also the most informative and reliable marker, which would reflect the evolutionary history of the genus, has not been found. The main achievements and problems of Fusarium phylogenetics, and perspectives of the application of molecular approach for studies of this group of organisms are discussed in this review.

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Fusarium culmorum is a single phylogenetic species based on multilocus sequence analysis

Cited by 46 publications

References 57 publications

Fusarium species and chemotypes associated with fusarium head blight and fusarium root rot on wheat in Sardinia

Fusarium species and chemotypes associated with fusarium head blight and fusarium root rot on wheat in Sardinia

Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum

MOLECULAR GENETIC APPROACHES FOR INVESTIGATION OF TAXONOMY AND SPECIFIC IDENTIFICATION OF TOXIN-PRODUCING Fusarium SPECIES: ACHIEVEMENTS AND PROBLEMS (review)

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