FUS3-dependent gene regulation during late embryogenesis

Reidt, Wim; Ellerström, Mats; Kölle, Kerstin; Tewes, Annegret; Tiedemann, Jens; Altschmied, Lothar; Bäumlein, Helmut

doi:10.1078/0176-1617-00352

Cited by 15 publications

(9 citation statements)

References 39 publications

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“…Our finding that NF-Y-B6 has a role in the signaling mechanism responsible for interfering with the ABA-mediated delay in seed germination, but does not seem to participate in the BLF system induction of Lhcb expression in the etiolated cotyledons is consistent with the previous observations regarding the relationship between NF-Y-B9 and NF-Y-B6 (Kwong et al, 2003). Other embryonic tissue-active proteins involved with LEC1 and LEC1 LIKE can be found in tissues other than seed, like FUS3 (Reidt et al, 2001), which may be involved in vegetative processes. ABI3 also is reported to be active in nonseed tissues including the shoot apex (Rohde et al, 1999;Kurup et al, 2000), as is ABI5 and ABF3 in seeds and seedling stress responses (Finkelstein et al, 2005).…”

Section: Hissupporting

confidence: 79%

The GCR1, GPA1, PRN1, NF-Y Signal Chain Mediates Both Blue Light and Abscisic Acid Responses in Arabidopsis

et al. 2007

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Different classes of biotic (e.g. plant hormones) and abiotic (e.g. different wavelengths of light) signals act through specific signal transduction mechanisms to coordinate higher plant development. While a great deal of progress has been made, full signal transduction chains have not yet been described for most blue light-or abscisic acid-mediated events. Based on data derived from T-DNA insertion mutants and yeast (Saccharomyces cerevisiae) two-hybrid and coprecipitation assays, we report a signal transduction chain shared by blue light and abscisic acid leading to light-harvesting chlorophyll a/b-binding protein expression in etiolated Arabidopsis (Arabidopsis thaliana) seedlings. The chain consists of GCR1 (the sole Arabidopsis protein coding for a potential G-protein-coupled receptor), GPA1 (the sole Arabidopsis Ga-subunit), Pirin1 (PRN1; one of four members of an iron-containing subgroup of the cupin superfamily), and a nuclear factor Y heterotrimer comprised of A5, B9, and possibly C9. We also demonstrate that this mechanism is present in imbibed seeds wherein it affects germination rate.

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Section: Hissupporting

confidence: 79%

The GCR1, GPA1, PRN1, NF-Y Signal Chain Mediates Both Blue Light and Abscisic Acid Responses in Arabidopsis

et al. 2007

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“…Transactivation and binding studies demonstrated that the FUS3 factor physically interacts with the RY element of seed-specific gene promoters (Reidt et al 2000). In transient-expression assays based on tobacco protoplasts, RY-containing seed-specific promoters can be strongly induced both by FUS3 and ABI3, either separately or in combination (Reidt et al 2000(Reidt et al , 2001.…”

Section: Discussionmentioning

confidence: 97%

“…This was shown for the legumin (Ba¨umlein et al 1992) and for the napin promoter Stalberg et al 1996). Transient-expression studies in protoplasts demonstrated the functional link between the RY motif and the B3 domain containing transcription factors such as PvALF, ABI3 and FUS3 (Bobb et al 1997;Ezcurra et al 2000;Reidt et al 2000Reidt et al , 2001. Moreover, the isolated B3 domain of the VP1 factor was found to bind specifically to the Sph/RY element TCCATGCAT involved in the VP1-mediated induction of the C1 gene (Suzuki et al 1997).…”

Section: Introductionmentioning

confidence: 88%

Seed-specific transcription factors ABI3 and FUS3: molecular interaction with DNA

Mönke

Altschmied

Tewes

et al. 2004

Planta

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162

141

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In Arabidopsis thaliana (L.) Heynh. the seed-specific transcription factors ABI3 and FUS3 have key regulatory functions during the development of mature seeds. The highly conserved RY motif [DNA motif CATGCA(TG)], present in many seed-specific promoters, is an essential target of both regulators. Here we show that, in vitro, the full-length ABI3 protein, as well as FUS3 protein, is able to bind to RY-DNA and that the B3 domains of both transcription factors are necessary and sufficient for the specific interaction with the RY element. Flanking sequences of the RY motif modulate the binding, but the presence of an RY sequence alone allows the specific interaction of ABI3 and FUS3 with the target in vitro. Transcriptional activity of ABI3 and FUS3, measured by transient promoter activation, requires the B3 DNA-binding domain and an activation domain. In addition to the known N-terminal-located activation domain, a second transcription activation domain was found in the B1 region of ABI3.

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“…In plant development the expression of these genes is related to zygotic embrygenesis, and declines during seed germination (Lotan et al, 1998;Reidt et al, 2001). LEC genes have also been found to be active during SE induced in IZE-derived culture, and LEC2 expression was stimulated in embryogenic culture (Gaj et al, 2005;Ledwoń and Gaj, 2009).…”

Section: Discussionmentioning

confidence: 99%

Immature Zygotic Embryo Cultures of Arabidopsis. A Model System for Molecular Studies on Morphogenic Pathways Induced In Vitro

Kraut¹,

Wójcikowska²,

Ledwoń³

et al. 2011

Acta Biologica Cracoviensia Series Botanica

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To understand the molecular mechanism controlling in vitro plant morphogenesis, a culture system enabling induction of alternative morphogenic pathways (somatic embryogenesis, SE; shoot organogenesis, ORG) in a well defined population of somatic cells is needed. Arabidopsis is the most useful model plant for genomic studies, but a system in which SE or ORG can be induced alternatively in the same type of explant has not been proposed. Immature zygotic embryos (IZEs) of Arabidopsis provide the only explants with embryogenic potential, and have been recommended for studying mechanisms of SE induced in vitro. This study was aimed at defining culture conditions promoting induction of alternative morphogenic pathways: shoot ORG in IZE explants. The established protocol involves pretreatment of IZE explants with liquid auxin-rich callus induction (CIM) medium, followed by subculture on solid cytokinin-rich shoot induction medium (SIM). The method enables efficient shoot induction in Columbia (Col-0) and Wassilewskija (Ws), genotypes commonly used in molecular studies. During 3 weeks of culture up to 90% of Col-0 and 70% of Ws explants regenerated shoots via an indirect morphogenic pathway. We analyzed the qRT-PCR expression patterns of the LEC (LEC1, LEC2 and FUS3) genes, the key regulators of Arabidopsis embryogenesis, in the IZE explants induced to promote shoot ORG. The sharp decline of LEC expression on SIM medium confirmed that culture of Arabidopsis IZE explants enables experimental manipulation of the morphogenic response of somatic cells. A scheme illustrating various in vitro morphogenic responses of IZEs in relation to hormonal treatment is presented.K Ke ey y w wo or rd ds s: : Arabidopsis, immature zygotic embryos, in vitro morphogenesis, shoot regeneration.

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FUS3-dependent gene regulation during late embryogenesis

Cited by 15 publications

References 39 publications

The GCR1, GPA1, PRN1, NF-Y Signal Chain Mediates Both Blue Light and Abscisic Acid Responses in Arabidopsis

The GCR1, GPA1, PRN1, NF-Y Signal Chain Mediates Both Blue Light and Abscisic Acid Responses in Arabidopsis

Seed-specific transcription factors ABI3 and FUS3: molecular interaction with DNA

Immature Zygotic Embryo Cultures of Arabidopsis. A Model System for Molecular Studies on Morphogenic Pathways Induced In Vitro

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