Seed-specific transcription factors ABI3 and FUS3: molecular interaction with DNA

Mönke, Gudrun; Altschmied, Lothar; Tewes, Annegret; Reidt, Wim; Mock, Hans‐Peter; Bäumlein, Helmut; Conrad, Udo

doi:10.1007/s00425-004-1206-9

Cited by 165 publications

(153 citation statements)

References 39 publications

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“…Recent studies have demonstrated that they directly controlled the induction of SSP gene expression, recognizing the RY motifs present in the promoters of these target genes Reidt et al, 2000;Kroj et al, 2003;Mö nke et al, 2004;Braybrook et al, 2006). Accordingly, we found that FUS3 is able to specifically bind the RY element of the SDH2-3 promoter in EMSA assays (Fig.…”

Section: B3 Domain Transcription Factors Regulate Sdh2-3supporting

confidence: 58%

“…FUS3 and ABI3 Bind the SDH2-3 Promoter RY motifs are putative targets for B3 domain transcription factors (Suzuki et al, 1997;Reidt et al, 2000;Kroj et al, 2003;Mö nke et al, 2004). To investigate whether these factors could recognize in vitro the SDH2-3 RY promoter element, ABI3 and FUS3 proteins expressed in Escherichia coli were tested for their ability to bind to an oligonucleotide containing this element in an electrophoretic mobility shift assay (EMSA).…”

Section: Abre and Ry Elements Are Required For Sdh2-3 Promoter Activitymentioning

confidence: 99%

“…Streptavidin-coated microwell strips (Nunc) were used, and the following double-stranded biotinylated DNA fragments were prepared according to Mö nke et al (2004): for the ABRE2-ABRE3 probe, ABRE2-ABRE3F and ABRE2-ABRE3R; and for the ABRE1-RY probe, ABRE1-RYF and ABRE1-RYR. Antibodies against T7 tag (Novagen) and GST (Amersham Biosciences) conjugated with horseradish peroxidase (HRP) were used to detect bound transcription factors.…”

Section: Dna-protein Interaction Analysis By Elisamentioning

confidence: 99%

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A Nuclear Gene Encoding the Iron-Sulfur Subunit of Mitochondrial Complex II Is Regulated by B3 Domain Transcription Factors during Seed Development in Arabidopsis

et al. 2009

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Mitochondrial complex II (succinate dehydrogenase) is part of the tricarboxylic acid cycle and the respiratory chain. Three nuclear genes encode its essential iron-sulfur subunit in Arabidopsis (Arabidopsis thaliana). One of them, SUCCINATE DEHYDROGENASE2-3 (SDH2-3), is specifically expressed in the embryo during seed maturation, suggesting that SDH2-3 may have a role as the complex II iron-sulfur subunit during embryo maturation and/or germination. Here, we present data demonstrating that three abscisic acid-responsive elements and one RY-like enhancer element, present in the SDH2-3 promoter, are involved in embryo-specific SDH2-3 transcriptional regulation. Furthermore, we show that ABSCISIC ACID INSENSI-TIVE3 (ABI3), FUSCA3 (FUS3), and LEAFY COTYLEDON2, three key B3 domain transcription factors involved in gene expression during seed maturation, control SDH2-3 expression. Whereas ABI3 and FUS3 interact with the RY element in the SDH2-3 promoter, the abscisic acid-responsive elements are shown to be a target for bZIP53, a member of the basic leucine zipper (bZIP) family of transcription factors. We show that group S1 bZIP53 protein binds the promoter as a heterodimer with group C bZIP10 or bZIP25. To the best of our knowledge, the SDH2-3 promoter is the first embryo-specific promoter characterized for a mitochondrial respiratory complex protein. Characterization of succinate dehydrogenase activity in embryos from two homozygous sdh2-3 mutant lines permits us to conclude that SDH2-3 is the major iron-sulfur subunit of mature embryo complex II. Finally, the absence of SDH2-3 in mutant seeds slows down their germination, pointing to a role of SDH2-3-containing complex II at an early step of germination.

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Section: B3 Domain Transcription Factors Regulate Sdh2-3supporting

confidence: 58%

Section: Abre and Ry Elements Are Required For Sdh2-3 Promoter Activitymentioning

confidence: 99%

Section: Dna-protein Interaction Analysis By Elisamentioning

confidence: 99%

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A Nuclear Gene Encoding the Iron-Sulfur Subunit of Mitochondrial Complex II Is Regulated by B3 Domain Transcription Factors during Seed Development in Arabidopsis

et al. 2009

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“…We found that six copies of the highly conserved RY motif CATGCA(TG), an essential target of FUS3 and ABI3 transcription factors (Mö nke et al, 2004), are present in the AtMYB44 promoter. ABI3 and FUS3 transcription factors are associated with ABA action (Nambara et al, 2000;Gazzarrini et al, 2004).…”

Section: Discussionmentioning

confidence: 97%

Overexpression of AtMYB44 Enhances Stomatal Closure to Confer Abiotic Stress Tolerance in Transgenic Arabidopsis

et al. 2007

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AtMYB44 belongs to the R2R3 MYB subgroup 22 transcription factor family in Arabidopsis (Arabidopsis thaliana). Treatment with abscisic acid (ABA) induced AtMYB44 transcript accumulation within 30 min. The gene was also activated under various abiotic stresses, such as dehydration, low temperature, and salinity. In transgenic Arabidopsis carrying an AtMYB44 promoterdriven b-glucuronidase (GUS) construct, strong GUS activity was observed in the vasculature and leaf epidermal guard cells. Transgenic Arabidopsis overexpressing AtMYB44 is more sensitive to ABA and has a more rapid ABA-induced stomatal closure response than wild-type and atmyb44 knockout plants. Transgenic plants exhibited a reduced rate of water loss, as measured by the fresh-weight loss of detached shoots, and remarkably enhanced tolerance to drought and salt stress compared to wild-type plants. Microarray analysis and northern blots revealed that salt-induced activation of the genes that encode a group of serine/threonine protein phosphatases 2C (PP2Cs), such as ABI1, ABI2, AtPP2CA, HAB1, and HAB2, was diminished in transgenic plants overexpressing AtMYB44. By contrast, the atmyb44 knockout mutant line exhibited enhanced salt-induced expression of PP2C-encoding genes and reduced drought/salt stress tolerance compared to wild-type plants. Therefore, enhanced abiotic stress tolerance of transgenic Arabidopsis overexpressing AtMYB44 was conferred by reduced expression of genes encoding PP2Cs, which have been described as negative regulators of ABA signaling.

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“…The B3 domains of ABI3, FUS3, and LEC2 exhibit binding to sequences containing the RY motif [CATGCA] (Suzuki et al, 1997;Kroj et al, 2003;Mö nke et al, 2004). It is not known at present whether the B3 domains of the HSI2 subfamily proteins exhibit binding to sequences similar to the RY motif.…”

Section: Discussionmentioning

confidence: 99%

Analysis of a Sugar Response Mutant of Arabidopsis Identified a Novel B3 Domain Protein That Functions as an Active Transcriptional Repressor

Tsukagoshi

Saijo²,

Shibata³

et al. 2005

Plant Physiology

106

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A recessive mutation hsi2 of Arabidopsis (Arabidopsis thaliana) expressing luciferase (LUC) under control of a short promoter derived from a sweet potato (Ipomoea batatas) sporamin gene (Spo min TLUC) caused enhanced LUC expression under both lowand high-sugar conditions, which was not due to increased level of abscisic acid. The hsi2 mutant contained a nonsense mutation in a gene encoding a protein with B3 DNA-binding domain. HSI2 and two other Arabidopsis proteins appear to constitute a novel subfamily of B3 domain proteins distinct from ABI3, FUS3, and LEC2, which are transcription activators involved in seed development. The C-terminal part of HSI2 subfamily proteins contained a sequence similar to the ERFassociated amphiphilic repression (EAR) motif. Deletion of the C-terminal portion of HSI2 lost in the hsi2 mutant caused reduced nuclear targeting of HSI2. Null allele of HSI2 showed even higher Spo min TLUC expression than the hsi2 mutant, whereas overexpression of HSI2 reduced the LUC expression. Transient coexpression of 35STHSI2 with Spo min TLUC in protoplasts repressed the expression of LUC activity, and deletion or mutation of the EAR motif significantly reduced the repression activity of HSI2. These results indicate that HSI2 and related proteins are B3 domain-EAR motif active transcription repressors.In addition to transcriptional activators, transcriptional repressors play important roles in the regulation of transcription. Transcriptional repressors are basically classified into passive repressors and active repressors (Hanna-Rose and Hansen, 1996;Thiel et al., 2004). Passive repressors do not have intrinsic repressing activity and inhibit the activation of transcription by inhibiting the function of transcriptional activators through a competition with activators for binding with DNA or a formation of inactive heterodimers with activators. On the other hand, active repressors inhibit transcription in an activatorindependent manner by binding with basic transcription factors or corepressors.The expression of a number of plant genes is regulated by changes in sugar status via multiple signal transduction pathways (for review, see Koch, 1996;Smeekens, 2000;Rolland et al., 2002). Genetic analyses of sugar signaling mutants of Arabidopsis (Arabidopsis thaliana), obtained by genetic screen based on inhibition of germination and early seedling development by sugars, revealed a close link between sugar signaling and the production of ethylene and abscisic acid (ABA; for review, see Gazzarrini and McCourt, 2001;Rook and Bevan, 2003). In addition, direct screening based on sugar-responsive expression of endogenous genes or transgenes with reporters also resulted in identification of mutants with altered sugar-responsive gene expression (Dijkwel et al., 1997;Martin et al., 1997;Mita et al., 1997aMita et al., , 1997bRook et al., 2001; Baier et al., 2004). In general, these mutants affected the expression of a subset of sugar-regulated genes, and mutation either diminished or enhanced the sugarresponsive ex...

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Seed-specific transcription factors ABI3 and FUS3: molecular interaction with DNA

Cited by 165 publications

References 39 publications

A Nuclear Gene Encoding the Iron-Sulfur Subunit of Mitochondrial Complex II Is Regulated by B3 Domain Transcription Factors during Seed Development in Arabidopsis

A Nuclear Gene Encoding the Iron-Sulfur Subunit of Mitochondrial Complex II Is Regulated by B3 Domain Transcription Factors during Seed Development in Arabidopsis

Overexpression of AtMYB44 Enhances Stomatal Closure to Confer Abiotic Stress Tolerance in Transgenic Arabidopsis

Analysis of a Sugar Response Mutant of Arabidopsis Identified a Novel B3 Domain Protein That Functions as an Active Transcriptional Repressor

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