Further study on the cytogenetic effects of combined chemotherapy with isoniazid and para‐aminosalicylic acid on human lymphocytes in vivo: Sister chromatid exchanges, chromosome aberrations in first‐division metaphases, cell growth kinetics, and mitotic index

Abstract

Tuberculosis patients under therapy with the drug combination isoniazid (INH) and para-aminosalicylic acid (PAS) were screened to investigate the cytogenetic effects. Results were compared with those from the patient controls (ie, tuberculosis patients before starting drug therapy) and general controls (individuals from the general population). The induction of chromosome aberrations was homogeneous and increased significantly in all the drug-exposed patients, whereas the frequency of sister chromatid exchanges (SCEs) was increased only in one of them. These results indicated differences in the two processes: chromosome aberrations and SCEs. Patients under therapy exhibited no change in cell growth kinetics, but the mitotic index was slightly enhanced compared to patient controls. It is concluded that the drug combination of INH + PAS induces damage in lymphocyte chromosomes in vivo. Further studies should be carried out on germ cells.

Screening of drug targets for tuberculosis on the basis of transcription factor regulatory network and mRNA sequencing technology

“…The GO analysis showed that the enrichment of the mitotic cell cycle was the most significant in the biological processes (BP), and it is well known that mitosis is an important process to maintain the normal growth and development of individuals. It has been indicated in some studies that the mitotic index of tuberculosis patients who have received treatment with anti-tuberculosis drugs will improve ( Jaju et al, 1983 ). In the cell components (CC), the enrichment of cytoplasm was the most significant.…”