Further studies on the relationship between platelet buoyant density and platelet age

Boneu, B; Vigoni, F.; Boneu, A; Caranobe, C; Sié, P.

doi:10.1002/ajh.2830130307

Cited by 27 publications

(14 citation statements)

References 16 publications

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“…These experiments were performed using similar techniques and experimental design to those previously reported in human studies [ 11,161. We observed that, although platelet density cohorts in dogs appear to be constituted by platelets of different ages, there is a net decrease in density with aging. These observations support earlier evidence in rabbits and rhesus monkeys [l-51 but contrast with the findings in human volunteers, baboons, and Macaca fasicularis monkeys [10][11][12][13][16][17][18]], suggesting that the direction of the change in buoyant density with age is dependent on the species studied.…”

Section: Introductionsupporting

confidence: 88%

“…Numerous studies performed during the past years have addressed the question of the relationship between platelet age and buoyant density with contradictory results. These investigations have been carried out using different methodologies and experimental approaches and have been performed in humans and in various animal species [1][2][3][4][5][6][7][8][9][10][11][12][13]. These facts, as well as the different interpretations of similar experimental findings [ 14,151, explain the prolongation of the controversy over the years.…”

Section: Introductionmentioning

confidence: 99%

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Kinetics of platelet density subpopulations in splenectomized mongrel dogs

et al. 1984

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Autologous 51Cr-platelet kinetic studies were performed in splenectomized mongrel dogs. Mean survival time of PRP-platelets was 5.4 +/- 1.5 (SD) days (n = 6). The curves, though slightly curvilinear, showed mostly a linear type of decay, denoting that platelet removal from the circulation is mainly determined by aging of the cells. High-density (HD) and low-density (LD) platelet cohorts were isolated in Stractan gradients from samples drawn daily after infusion of labeled platelets. Specific radioactivity in HD cohorts declined rapidly postinfusion (T1/2 = 1.3 days), but specific radioactivity in LD platelets increased for 2 days and steadily declined for 4 days thereafter (n = 6). Labeled HD platelets, comprising 11.7% of the total population, lived significantly longer in circulation than LD platelets (19.1% of the total population) (n = 3). The patterns of decay of the radioactivity, however, do not have all the characteristics of pure age-cohort survival curves; 3.7 days after the infusion of labeled HD platelets, the specific radioactivity in LD cohorts was six times higher than on day 1, but attained only 20% of the initial specific radioactivity in HD platelets. After the infusion of labeled LD platelets no radioactivity was recovered in circulating HD cohorts. These findings indicate that mongrel dog platelets decrease in density with aging, but also that platelet density heterogeneity is in part determined during the thrombopoietic process. These data are consistent with those of other authors in rabbits and rhesus monkeys, but contrast with the observations that platelets in humans, baboons, and Macaca fascicularis monkeys increase in density with age, suggesting that the displacement of platelets toward compartments of either higher or lower density depends on the species under study.

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Section: Introductionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Kinetics of platelet density subpopulations in splenectomized mongrel dogs

et al. 1984

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“…Some authors take the view that density decreases as platelets circulate [9,10] . Other studies suggest that recently created platelets are less dense and that density increases with age [11][12][13] . Finally, evidence exists indicating that platelet density does not change in vivo [14][15][16] .…”

mentioning

confidence: 99%

Platelet Density Distribution in Essential Thrombocythemia

Milovanovic

Lotfi

Lindahl

et al. 2010

Pathophysiol Haemos Thromb

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Essential thrombocythemia (ET) is characterized by high platelet counts and a slightly increased bleeding risk. Why severe hemorrhage does not occur more frequently is not known. Variations of platelet density (kg/l) depend mainly on cell organelle content in that high-density platelets contain more α and dense granules. This study compares ET patients (n = 2) and healthy volunteers (n = 2) with respect to platelet density subpopulations. A linear Percoll™ gradient containing prostaglandin E₁ was employed to separate platelets according to density. The platelet population was subsequently divided by density into 16 or 17 subpopulations. Determination of platelet counts was carried out. In each density fraction, platelet in vivo activity, i.e. platelet-bound fibrinogen, was measured using a flow cytometer. To further characterize platelet subpopulations, we determined intracellular concentrations of CD40 ligand (CD40L) and P-selectin in all fractions. Patients and controls demonstrated similar density distributions, i.e. 1 density peak. High-density platelets had more surface-bound fibrinogen in conjunction with signs of platelet release reactions, i.e. with few exceptions they contained less CD40L and P-selectin. Peak density platelets showed less surface-bound fibrinogen. These platelets contained less CD40L and P-selectin than nearby denser populations. The light platelets had more surface-bound fibrinogen than peak platelets together with elevated concentrations of CD40L. In ET, the malignant platelet production could exist together with platelets originating from normal megakaryocytes. It is also possible that clonal megakaryocytes produce platelets covering the entire density span. The ‘normal’ density distribution offers a tenable explanation as to why serious bleedings do not occur more frequently.

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“…Recent suggestions, that young blood platelets increase their buoyant density during the first several days in the circulation by an accumulation of different substances in granules (264)(265)(266)(267), if confirmed, would lend further support to the lysosomal concept of platelet secretory granules. Obviously platelet density may be perceived as a dynamic equilibrium between endocytosis and exocytosis.…”

Section: A Lysosomal Concept Of Platelet Secretionmentioning

confidence: 81%

Lysosomal Consept of Platelet Secretion ‐ Revisited

Polasek

1989

European J of Haematology

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Certain morphological and biochemical aspects of platelet secretion are discussed. Based on own experiments and review of the literature a hypothesis is forwarded that platelet secretory granules or rather storage organelles can be viewed as secondary lysosomes participating in platelet endocytosis and exocytosis. Formation of the platelet thromboplastic activity, so called PF3, is linked to the platelet storage organelles disintegration and lypolysis during their exocytosis through the platelet plasma membrane.

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Further studies on the relationship between platelet buoyant density and platelet age

Cited by 27 publications

References 16 publications

Kinetics of platelet density subpopulations in splenectomized mongrel dogs

Kinetics of platelet density subpopulations in splenectomized mongrel dogs

Platelet Density Distribution in Essential Thrombocythemia

Lysosomal Consept of Platelet Secretion ‐ Revisited

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