2010
DOI: 10.1159/000314964
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Platelet Density Distribution in Essential Thrombocythemia

Abstract: Essential thrombocythemia (ET) is characterized by high platelet counts and a slightly increased bleeding risk. Why severe hemorrhage does not occur more frequently is not known. Variations of platelet density (kg/l) depend mainly on cell organelle content in that high-density platelets contain more α and dense granules. This study compares ET patients (n = 2) and healthy volunteers (n = 2) with respect to platelet density subpopulations. A linear Percoll™ gradient containing prostaglandin E1 was em… Show more

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Cited by 16 publications
(17 citation statements)
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“…These findings are in agreement with those from early studies using platelet morphology in blood smears or other Hematology analyzers [21][22][23][24]. The increase in platelet volume and the decrease in platelet buoyant density that we observed could be explained by proliferation or dilation of the dense tubular and open canalicular system and reduction of the mitochondria, dense granules, and -granules, as found in ultrastructural studies of platelets from ET patients [25][26][27][28]. Presumably, the origin of the above morphologic findings may be an aberrant proplatelet formation and abnormalities in ploidy, growth rates, and demarcation during the clonal megakaryocytopoiesis of ET, although enhanced platelet activation in the circulation would also be a possibility [2,3,[26][27][28][29][30].…”
Section: Discussionsupporting
confidence: 92%
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“…These findings are in agreement with those from early studies using platelet morphology in blood smears or other Hematology analyzers [21][22][23][24]. The increase in platelet volume and the decrease in platelet buoyant density that we observed could be explained by proliferation or dilation of the dense tubular and open canalicular system and reduction of the mitochondria, dense granules, and -granules, as found in ultrastructural studies of platelets from ET patients [25][26][27][28]. Presumably, the origin of the above morphologic findings may be an aberrant proplatelet formation and abnormalities in ploidy, growth rates, and demarcation during the clonal megakaryocytopoiesis of ET, although enhanced platelet activation in the circulation would also be a possibility [2,3,[26][27][28][29][30].…”
Section: Discussionsupporting
confidence: 92%
“…The reduced MPC and the elevated PCDW found in ET patients are consistent with the degranulation and shape changes that occur during the process of platelet activation [9,25,27,28]. It must be noted that, in agreement with other experimental and clinical data [4,8,9,25, 27, 28, 31, 32], we observed a negative correlation with moderate intensity of concordance between the percentage of baseline P-selectin expression and the MPC.…”
Section: Discussionsupporting
confidence: 90%
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“…Venous blood (7.5 mL) was anticoagulated with 2.5 mL 0.129 M disodium citrate. In order to separate platelets according to density a linear Percoll™ (GE Healthcare Bio-Sciences AB, Sweden) gradient was used [29,30]. The following substances were mixed in order to provide the two Percoll™ solutions (1.09 and 1.04 kg/L) for the gradient ( Table 2).…”
Section: Laboratory Investigationsmentioning
confidence: 99%
“…The tube was thereafter centrifuged at 2767 g for 1½ hours. After centrifugation, the underside of the test tube was punctured and the contents was separated by gravity into 17 different density fractions [30]. By this setting every fraction holds about 2 mL of the test tube content.…”
Section: Laboratory Investigationsmentioning
confidence: 99%