Further structural characterization of ovine forestomach matrix and multi-layered extracellular matrix composites for soft tissue repair

Smith, Matthew J.; Dempsey, Sandi G.; Veale, Robert W. F.; Duston-Fursman, Claudia G.; Rayner, Chloe A F; Javanapong, Chettha; Gerneke, Dane; Dowling, Shane G.; Bosque, Brandon A; Karnik, Tanvi; Jerram, Michael J; Nagarajan, Anantha Venkataraman; Rajam, Ravinder; Jowsey, Alister T; Cutajar, Samuel; Mason, Isaac; Stanley, Roderick G; Malmström, Jenny; Miller, Chris H.; May, Barnaby C. H.

doi:10.1177/08853282211045770

Cited by 11 publications

(6 citation statements)

References 78 publications

(116 reference statements)

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“…Lyophilized hydrogel sponges usually allow for improved cell migration and flow of nutrients into the interiors of the scaffold, depending on the pore structure. Another method to create composite, multilayer scaffolds is to fabricate an interlocking hole-and-tab system to interlock sheets of biomaterials, without the need to add cross-linking chemicals [ 295 ].…”

Section: Ecm Modification and Methodsmentioning

confidence: 99%

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

McInnes

Möser

Chen

2022

JFB

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The multidisciplinary fields of tissue engineering and regenerative medicine have the potential to revolutionize the practise of medicine through the abilities to repair, regenerate, or replace tissues and organs with functional engineered constructs. To this end, tissue engineering combines scaffolding materials with cells and biologically active molecules into constructs with the appropriate structures and properties for tissue/organ regeneration, where scaffolding materials and biomolecules are the keys to mimic the native extracellular matrix (ECM). For this, one emerging way is to decellularize the native ECM into the materials suitable for, directly or in combination with other materials, creating functional constructs. Over the past decade, decellularized ECM (or dECM) has greatly facilitated the advance of tissue engineering and regenerative medicine, while being challenged in many ways. This article reviews the recent development of dECM for tissue engineering and regenerative medicine, with a focus on the preparation of dECM along with its influence on cell culture, the modification of dECM for use as a scaffolding material, and the novel techniques and emerging trends in processing dECM into functional constructs. We highlight the success of dECM and constructs in the in vitro, in vivo, and clinical applications and further identify the key issues and challenges involved, along with a discussion of future research directions.

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Section: Ecm Modification and Methodsmentioning

confidence: 99%

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

McInnes

Möser

Chen

2022

JFB

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“…Sugar-induced modification of the decellularized kidneys increases mechanical strength and resistance to deformation ( Sant et al, 2021 ). Recently multilayered decellularized scaffolds demonstrated promising bioactivity and should be further studied to be widely used ( Smith et al, 2022 ). Moreover, growth factors and drugs could be loaded on the dECMs, making them more potent.…”

Section: Pre-application Processingmentioning

confidence: 99%

Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods

Neishabouri

Khaboushan

Daghigh

et al. 2022

Front. Bioeng. Biotechnol.

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Reproduction of different tissues using scaffolds and materials is a major element in regenerative medicine. The regeneration of whole organs with decellularized extracellular matrix (dECM) has remained a goal despite the use of these materials for different purposes. Recently, decellularization techniques have been widely used in producing scaffolds that are appropriate for regenerating damaged organs and may be able to overcome the shortage of donor organs. Decellularized ECM offers several advantages over synthetic compounds, including the preserved natural microenvironment features. Different decellularization methods have been developed, each of which is appropriate for removing cells from specific tissues under certain conditions. A variety of methods have been advanced for evaluating the decellularization process in terms of cell removal efficiency, tissue ultrastructure preservation, toxicity, biocompatibility, biodegradability, and mechanical resistance in order to enhance the efficacy of decellularization methods. Modification techniques improve the characteristics of decellularized scaffolds, making them available for the regeneration of damaged tissues. Moreover, modification of scaffolds makes them appropriate options for drug delivery, disease modeling, and improving stem cells growth and proliferation. However, considering different challenges in the way of decellularization methods and application of decellularized scaffolds, this field is constantly developing and progressively moving forward. This review has outlined recent decellularization and sterilization strategies, evaluation tests for efficient decellularization, materials processing, application, and challenges and future outlooks of decellularization in regenerative medicine and tissue engineering.

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“…However, these models fail to fully capture the physiological architecture or stiffness of the native tendon tissue simultaneously. ,− Tissue culture plates, which are commonly used for in vitro studies, expose the cells to a highly stiff, flat substrate that does not resemble the tendon microenvironment and causes a loss of tenogenic phenotype. , In contrast, aligned tendon matrix models prepared by electrospinning polycaprolactone or anisotropic collagen gels fail to incorporate the native tendon stiffness and do not mimic the tendon fibrillar architecture. ,,− , Therefore, in vitro models that manage to capture the complexity of the native tissue at scale for stiffness and architecture would be a valuable tool for studying cell behavior in healthy and diseased tendon matrices. Decellularized matrices closely mimic the in vivo tissue characteristics and thereby offer an ideal platform as an in vitro tissue mimetic model. − Although there have been previous studies that have decellularized tendons for tissue engineering purposes, − to our knowledge, this is the first study to utilize decellularized tendons as an in vitro model, and further characterizing cell phenotype is yet to be explored.…”

Section: Introductionmentioning

confidence: 99%

Novel In Vitro Platform for Studying the Cell Response to Healthy and Diseased Tendon Matrices

Konar,

Leung,

Tay

et al. 2024

ACS Biomater. Sci. Eng.

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Current in vitro models poorly represent the healthy or diseased tendon microenvironment, limiting the translation of the findings to clinics. The present work aims to establish a physiologically relevant in vitro tendon platform that mimics biophysical aspects of a healthy and tendinopathic tendon matrix using a decellularized bovine tendon and to characterize tendon cells cultured using this platform. Bovine tendons were subjected to various decellularization techniques, with the efficacy of decellularization determined histologically. The biomechanical and architectural properties of the decellularized tendons were characterized using an atomic force microscope. Tendinopathy-mimicking matrices were prepared by treating the decellularized tendons with collagenase for 3 h or collagenase−chondroitinase (CC) for 1 h. The tendon tissue collected from healthy and tendinopathic patients was characterized using an atomic force microscope and compared to that of decellularized matrices. Healthy human tendon-derived cells (hTDCs) from the hamstring tendon were cultured on the decellularized matrices for 24 or 48 h, with cell morphology characterized using f-actin staining and gene expression characterized using real-time PCR. Tendon matrices prepared by freeze−thawing and 48 h nuclease treatment were fully decellularized, and the aligned structure and tendon stiffness (1.46 MPa) were maintained. Collagenase treatment prepared matrices with a disorganized architecture and reduced stiffness (0.75 MPa), mimicking chronic tendinopathy. Treatment with CC prepared matrices with a disorganized architecture without altering stiffness, mimicking early tendinopathy (1.52 MPa). hTDCs on a healthy tendon matrix were elongated, and the scleraxis (SCX) expression was maintained. On tendinopathic matrices, hTDCs had altered morphological characteristics and lower SCX expression. The expression of genes related to actin polymerization, matrix degradation and remodeling, and immune cell invasion were higher in hTDCs on tendinopathic matrices. Overall, the present study developed a physiological in vitro system to mimic healthy tendons and early and late tendinopathy, and it can be used to better understand tendon cell characteristics in healthy and diseased states.

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Further structural characterization of ovine forestomach matrix and multi-layered extracellular matrix composites for soft tissue repair

Cited by 11 publications

References 78 publications

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods

Novel In Vitro Platform for Studying the Cell Response to Healthy and Diseased Tendon Matrices

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