Further stabilization of lipase from Pseudomonas fluorescens immobilized on octyl coated nanoparticles via chemical modification with bifunctional agents

Rios, Nathália Saraiva; Morais, Eva Gomes; Galvão, Wesley S.; Neto, Davino M. Andrade; Santos, José Cleiton Sousa dos; Bohn, F.; Corrêa, M.A.; Fechine, Pierre Basílio Almeida; Fernández-Lafuente, Roberto; Gonçalves, Luciana Rocha Barros

doi:10.1016/j.ijbiomac.2019.09.003

Cited by 59 publications

(20 citation statements)

References 120 publications

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“…Covalent bonding is considered one of the strongest chemical interactions between enzymes and supports [86][87][88][89][90][91][92][93][94][95][96]. This immobilization process involves several enzyme residues.…”

Section: Covalent Linkagementioning

confidence: 99%

A Comprehensive Review on the Use of Metal–Organic Frameworks (MOFs) Coupled with Enzymes as Biosensors

et al. 2022

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Several studies have shown the development of electrochemical biosensors based on enzymes immobilized in metal–organic frameworks (MOFs). Although enzymes have unique properties, such as efficiency, selectivity, and environmental sustainability, when immobilized, these properties are improved, presenting significant potential for several biotechnological applications. Using MOFs as matrices for enzyme immobilization has been considered a promising strategy due to their many advantages compared to other supporting materials, such as larger surface areas, higher porosity rates, and better stability. Biosensors are analytical tools that use a bioactive element and a transducer for the detection/quantification of biochemical substances in the most varied applications and areas, in particular, food, agriculture, pharmaceutical, and medical. This review will present novel insights on the construction of biosensors with materials based on MOFs. Herein, we have been highlighted the use of MOF for biosensing for biomedical, food safety, and environmental monitoring areas. Additionally, different methods by which immobilizations are performed in MOFs and their main advantages and disadvantages are presented.

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Section: Covalent Linkagementioning

confidence: 99%

A Comprehensive Review on the Use of Metal–Organic Frameworks (MOFs) Coupled with Enzymes as Biosensors

et al. 2022

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“…Despite its excellent performance potential, industrial applications were made impossible due to these undesirable characteristics [195,196]. In this scenario, immobilization techniques stand out as alternatives to overcome these limitations, since they can offer better stability, increased activity and selectivity, excellent resistance, improvements in product separation and purification, and the possibility of enzyme reuse, rendering processes increasingly efficient [192][193][194][195][196][197][198][199][200][201][202][203][204][205][206].…”

Section: Enzymesmentioning

confidence: 99%

“…Enzymatic immobilization is based on the confinement of enzyme molecules on the surface of a reliable support that is different from that in which the substrate or products are present [202][203][204][205][206][207][208][209]. In contrast with their solubilized form, immobilized enzymes provide a large enzyme to substrate ratio, efficient digestion, and secure handling, in addition to showing more significant activity and the possibility of reuse for several cycles [197,[210][211][212][213].…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

et al. 2021

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Among the many biological entities employed in the development of biosensors, enzymes have attracted the most attention. Nanotechnology has been fostering excellent prospects in the development of enzymatic biosensors, since enzyme immobilization onto conductive nanostructures can improve characteristics that are crucial in biosensor transduction, such as surface-to-volume ratio, signal response, selectivity, sensitivity, conductivity, and biocatalytic activity, among others. These and other advantages of nanomaterial-based enzymatic biosensors are discussed in this work via the compilation of several reports on their applications in different industrial segments. To provide detailed insights into the state of the art of this technology, all the relevant concepts around the topic are discussed, including the properties of enzymes, the mechanisms involved in their immobilization, and the application of different enzyme-derived biosensors and nanomaterials. Finally, there is a discussion around the pressing challenges in this technology, which will be useful for guiding the development of future research in the area.

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“…Furthermore, regarding their application, free-state lipases (or free enzymes in general) have limitations such as sensitivity to the reaction medium and low operational stability, making their industrial use practical only by their immobilization [44][45][46]. Immobilization methods are good alternatives that can favor enzyme activity, facilitate biocatalyst recovery, modulate its selectivity and specificity, and improve resistance to inhibitors [44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59].…”

Section: Introductionmentioning

confidence: 99%

Current Status and Future Perspectives of Supports and Protocols for Enzyme Immobilization

Cavalcante

Sousa

et al. 2021

Catalysts

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The market for industrial enzymes has witnessed constant growth, which is currently around 7% a year, projected to reach $10.5 billion in 2024. Lipases are hydrolase enzymes naturally responsible for triglyceride hydrolysis. They are the most expansively used industrial biocatalysts, with wide application in a broad range of industries. However, these biocatalytic processes are usually limited by the low stability of the enzyme, the half-life time, and the processes required to solve these problems are complex and lack application feasibility at the industrial scale. Emerging technologies create new materials for enzyme carriers and sophisticate the well-known immobilization principles to produce more robust, eco-friendlier, and cheaper biocatalysts. Therefore, this review discusses the trending studies and industrial applications of the materials and protocols for lipase immobilization, analyzing their advantages and disadvantages. Finally, it summarizes the current challenges and potential alternatives for lipases at the industrial level.

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Further stabilization of lipase from Pseudomonas fluorescens immobilized on octyl coated nanoparticles via chemical modification with bifunctional agents

Cited by 59 publications

References 120 publications

A Comprehensive Review on the Use of Metal–Organic Frameworks (MOFs) Coupled with Enzymes as Biosensors

A Comprehensive Review on the Use of Metal–Organic Frameworks (MOFs) Coupled with Enzymes as Biosensors

Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

Current Status and Future Perspectives of Supports and Protocols for Enzyme Immobilization

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