Further observations on the production of hydrogen peroxide by anaerobic bacteria

Gordon, J.; Holman, R.A.; McLeod, J. W.

doi:10.1002/path.1700660224

Cited by 54 publications

(10 citation statements)

References 6 publications

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“…However, the thiol peroxidase is rather dispensable for the aerobic growth on glycerol. Early studies on NADH peroxidase predicted that one of its physiological role may be the scavenging of the H 2 O 2 formed by glycerol‐3‐phosphate oxidase (Gordon et al ., 1953). If this is correct, we supposed that in the Δ npr mutant, H 2 O 2 would accumulate, which should become gradually growth inhibitory during culture on this substrate.…”

Section: Discussionmentioning

confidence: 99%

“…Survey of the TIGR database revealed that the presence of genes encoding NADH peroxidase is restricted to few eubacterial genera, such as Streptococcus , Lactobacillus and Listeria . The presumed function of NADH peroxidase is to inactivate endogenous H 2 O 2 formed, for example by α‐glycerophosphate oxidase during glycerol metabolism or dismutation of O 2 ‐ , but the enzyme may also protect against exogenous H 2 O 2 (Gordon et al ., 1953). This activity is greatly induced by aerobic growth, and regulation of expression of the npr gene by an OxyR‐like enterococcal protein has been suggested (Pugh and Knowles, 1982; Ross and Claiborne, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Comparative study of the physiological roles of three peroxidases (NADH peroxidase, Alkyl hydroperoxide reductase and Thiol peroxidase) in oxidative stress response, survival inside macrophages and virulence of Enterococcus faecalis

Carbona¹,

Sauvageot²,

Giard³

et al. 2007

Molecular Microbiology

130

122

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SummaryThe opportunistic pathogen Enterococcus faecalis is well equipped with peroxidatic activities. It harbours three loci encoding a NADH peroxidase, an alkyl hydroperoxide reductase and a protein (EF2932) belonging to the AhpC/TSA family. We present results demonstrating that ef2932 does encode a thiol peroxidase (Tpx) and show that it is part of the regulon of the hydrogen peroxide regulator HypR. Characterization of unmarked deletion mutants showed that all three peroxidases are important for the defence against externally provided H 2O2. Exposure to internal generated H2O2 by aerobic growth on glycerol, lactose, galactose or ribose showed that Npr was absolutely required for aerobic growth on glycerol and optimal growth on the other substrates. Growth on glycerol was also dependent on Ahp. Addition of catalase restored growth of the mutants, and therefore, extracellular H2O2 concentrations have been determined. This showed that the time point of growth arrest of the Dnpr mutant correlated with the highest H2O2 concentration measured. Analysis of the survival of the different strains inside peritoneal macrophages revealed that Tpx was the most important antioxidant activity for protecting the cells against the hostile phagocyte environment. Finally, the Dtpx and the triple mutant showed attenuated virulence in a mouse peritonitis model.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative study of the physiological roles of three peroxidases (NADH peroxidase, Alkyl hydroperoxide reductase and Thiol peroxidase) in oxidative stress response, survival inside macrophages and virulence of Enterococcus faecalis

Carbona¹,

Sauvageot²,

Giard³

et al. 2007

Molecular Microbiology

130

122

View full text Add to dashboard Cite

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“…Technical limitations at the time prevented detection of the low but toxic concentrations of H202 that anaerobes produce. It was, however, subsequently shown that nearly all anaerobes do produce H202 (6). The sensitivity of anaerobes to H202 was readily shown, and a high degree of variation was apparent (4, 7).…”

mentioning

confidence: 99%

An Enzyme-Based Theory of Obligate Anaerobiosis: The Physiological Function of Superoxide Dismutase

McCord

Keele

Fridovich

1971

Proc. Natl. Acad. Sci. U.S.A.

844

394

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“…It is important to note that the equipment described is capable of controlling the dissolved oxygen tension well below the levels which can be tolerated by such ' strict anaerobes' as Clostridium tetani ( 2 mm. Hg, 0,; Gordon, Holman & McLeod, 1953).…”

Section: Discussionmentioning

confidence: 99%

Automatic Control of Dissolved Oxygen Concentration in Stirred Microbial Cultures

Maclennan¹,

Pirt²

1966

Journal of General Microbiology

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SUMMARYEquipment was developed for the automatic control of dissolved oxygen concentration in microbial cultures, by using the Mackereth electrode as a sensing probe. Factors affecting the choice of components are discussed and the performance of the equipment is described during typical batch and continuous culture runs. The equipment provided precise and reliable control over a wide range of dissolved oxygen concentrations, and retained a high degree of stability over long periods of continuous use.

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Further observations on the production of hydrogen peroxide by anaerobic bacteria

Cited by 54 publications

References 6 publications

Comparative study of the physiological roles of three peroxidases (NADH peroxidase, Alkyl hydroperoxide reductase and Thiol peroxidase) in oxidative stress response, survival inside macrophages and virulence of Enterococcus faecalis

Comparative study of the physiological roles of three peroxidases (NADH peroxidase, Alkyl hydroperoxide reductase and Thiol peroxidase) in oxidative stress response, survival inside macrophages and virulence of Enterococcus faecalis

An Enzyme-Based Theory of Obligate Anaerobiosis: The Physiological Function of Superoxide Dismutase

Automatic Control of Dissolved Oxygen Concentration in Stirred Microbial Cultures

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