Further Contribution to the Taxonomy of the «<i>Dugesia Lugubris-Polychroa Group</i>» with Description of<i>Dugesia Mediterranea</i>N.SP. (Tricladida, Paludicola)

Benazzi, Mario; Baguñà, Jaume; Ballester, Rafael; Puccinelli, Ileana; Papa, Roberto

doi:10.1080/11250007509430132

Cited by 58 publications

(58 citation statements)

References 5 publications

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“…To assay for the effects exerted by the exogenously introduced myosin dsRNA, we used the mAb TMUS-13 (which recognizes planarian myosin heavy chain) to visualize the body-wall musculature (21). dsRNA was synthesized as described (13) by using a 1,186-bp myosin cDNA clone isolated from the diploid, asexual strain of the planarian Schmidtea mediterranea (22). To deliver the myosin dsRNA, individuals of S. mediterranea were cut transversely into 5-6 pieces, and Ϸ32 nl of a solution containing 10 9 -10 11 molecules of myosin dsRNA were injected into the parenchyma and/or the gastrovascular system.…”

Section: Resultsmentioning

confidence: 99%

Double-stranded RNA specifically disrupts gene expression during planarian regeneration

Alvarado

Newmark²

1999

Proc. Natl. Acad. Sci. U.S.A.

474

259

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Metazoan regeneration is one of the least understood fundamental problems of biology. The lack of progress in understanding this phenomenon at the molecular level has been due to the poor regenerative abilities of the genetic organisms used for developmental studies, as well as the difficulties encountered with molecular and genetic manipulations of the commonly studied vertebrate models (the urodele amphibians). Here, we demonstrate that introduction of double-stranded RNA selectively abrogates gene function in planarians, a classic model of regeneration. The ability to eliminate gene function in a regenerating organism such as the planarian overcomes previous experimental limitations and opens the study of animal regeneration to unprecedented levels of molecular detail.

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Section: Resultsmentioning

confidence: 99%

Double-stranded RNA specifically disrupts gene expression during planarian regeneration

Alvarado

Newmark²

1999

Proc. Natl. Acad. Sci. U.S.A.

474

259

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“…Benazzi, Benazzi-Lentati, 1976). Benazzi recognised the existence of 3 species: D. polychroa (including biotypes A,B,C and D), D. lugubris (E,F) and D. mediterranea (biotype G) (Benazzi et al, 1975). Later, the new species D. nova was described for biotype F (Benazzi 1982).…”

Section: Schmidtea (Ball 1974)mentioning

confidence: 99%

Evolutionary history of the Tricladida and the Platyhelminthes: an up-to-date phylogenetic and systematic account

et al. 2012

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Within the free-living platyhelminths, the triclads, or planarians, are the best-known group, largely as a result of long-standing and intensive research on regeneration, pattern formation and Hox gene expression. However, the group's evolutionary history has been long debated, with controversies ranging from their phyletic structure and position within the Metazoa to the relationships among species within the Tricladida. Over the the last decade, with the advent of molecular phylogenies, some of these issues have begun to be resolved. Here, we present an upto-date summary of the main phylogenetic changes and novelties with some comments on their evolutionary implications. The phylum has been split into two groups, and

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“…Schmidtea mediterranea Benazzi, Baguñà , Ballester, and Del Papa, 1975 is restricted to the western Mediterranean in several scattered populations along the Catalan coast, Menorca, Mallorca, Corsica, Sardinia, Sicily and Tunisia [19][20][21]. There is an asexual strain that occurs only in a few locations, in Catalonia and the Balearic Islands, where sexual populations have not been found.…”

Section: Species Genetic Structure: Schmidtea Mediterranea and Dugesimentioning

confidence: 99%

Planarian (Platyhelminthes, Tricladida) Diversity and Molecular Markers: A New View of an Old Group

Álvarez‐Presas

Riutort

2014

Diversity

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Planarians are a group of free-living platyhelminths (triclads) best-known largely due to long-standing regeneration and pattern formation research. However, the group's diversity and evolutionary history has been mostly overlooked. A few taxonomists have focused on certain groups, resulting in the description of many species and the establishment of higher-level groups within the Tricladida. However, the scarcity of morphological features precludes inference of phylogenetic relationships among these taxa. The incorporation of molecular markers to study their diversity and phylogenetic relationships has facilitated disentangling many conundrums related to planarians and even allowed their use as phylogeographic model organisms. Here, we present some case examples ranging from delimiting species in an integrative style, and barcoding them, to analysing their evolutionary history on a lower scale to infer processes affecting biodiversity origin, or on a higher scale to understand the genus level or even higher relationships. In many cases, these studies have allowed proposing better classifications and resulted in taxonomical changes. We also explain shortcomings resulting in a lack of resolution or power to apply the most up-to-date data analyses. Next-generation sequencing methodologies may help improve this situation and accelerate their use as model organisms.

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Further Contribution to the Taxonomy of the «Dugesia Lugubris-Polychroa Group» with Description ofDugesia MediterraneaN.SP. (Tricladida, Paludicola)

Cited by 58 publications

References 5 publications

Double-stranded RNA specifically disrupts gene expression during planarian regeneration

Double-stranded RNA specifically disrupts gene expression during planarian regeneration

Evolutionary history of the Tricladida and the Platyhelminthes: an up-to-date phylogenetic and systematic account

Planarian (Platyhelminthes, Tricladida) Diversity and Molecular Markers: A New View of an Old Group

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