Fungicidal activity of Thai medicinal plant extracts against Alternaria brassicicola causing black spot of Chinese kale

Dethoup, Tida; Songkumarn, Patcharavipha; Rueangrit, Soraya; Suesa-ard, Supaporn; Kaewkrajay, Chutima

doi:10.1007/s10658-018-1460-5

Cited by 15 publications

(16 citation statements)

References 32 publications

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“…Moreover, numerous phenolic acids and phenolic-rich extracts exhibited high inhibitory effects on the radial growth of fungi [18,47]. Although considerable attempts have been made using naturally phenolic-rich products against Alternaria alternata [19][20][21][22][48][49][50][51], very few reports are available about their roles against A. solani. Nevertheless, the physiological and biochemical mechanisms behind this role are poorly understood.…”

Section: Discussionmentioning

confidence: 99%

The Antifungal Activity of Gallic Acid and Its Derivatives against Alternaria solani, the Causal Agent of Tomato Early Blight

et al. 2020

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Tomato (Solanum lycopersicum L.) is among the most important vegetable crops worldwide. Early blight disease, caused by Alternaria solani, is a destructive foliar disease of tomato and other Solanaceae species. Herein, we investigated the in vitro antifungal properties of gallic acid and two of its derivatives (syringic and pyrogallic acids) against A. solani during 2019 and 2020 seasons. The physiological and biochemical effects of these compounds on infected tomato plants were also investigated using the whole plant bioassay. The in vitro investigation showed that all tested compounds showed fungistatic action and inhibited the mycelial radial growth of A. solani in a dose-dependent manner. In two separate pot-experiments, those compounds efficiently suppressed the development of the disease symptoms and area under disease progress curve (AUDPC), without any phytotoxic effects on the treated tomato plants. Additionally, all tested compounds positively enhanced the biochemical traits of treated plants including the chlorophyll content, the total soluble phenolics, the total soluble flavonoids, and the enzymatic activities of catalase, peroxidase, and polyphenol oxidase during 2019 and 2020 seasons. Moreover, the treatment with gallic acid and its derivatives significantly increased all yield components of A. solani-infected tomato plants such as the total number of flowers and fruits, and the fruit yield for each tomato plant in both experiments. Considering the fungitoxicity of phenolic acids against A. solani with no phytotoxicity on treated tomato plants, we believe that gallic acid and its derivatives might be a sustainable eco-friendly control strategy to reduce the usage of chemical fungicides partially or entirely against A. solani particularly, and fungal diseases in general.

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Section: Discussionmentioning

confidence: 99%

The Antifungal Activity of Gallic Acid and Its Derivatives against Alternaria solani, the Causal Agent of Tomato Early Blight

et al. 2020

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“…The isolates were submerged in 10.0% glycerol and kept at 4 °C before use. All isolates were activated on the prepared potato dextrose agar (PDA) and incubated at room temperature (28 ± 2 °C) for 7 days before being used [ 64 ].…”

Section: Methodsmentioning

confidence: 99%

Recovery of Polyphenolic Fraction from Arabica Coffee Pulp and Its Antifungal Applications

Sangta

Wongkaew

Tangpao

et al. 2021

Plants

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Coffee pulp is one of the most underutilised by-products from coffee processing. For coffee growers, disposing of this agro-industrial biomass has become one of the most difficult challenges. This study utilised this potential biomass as raw material for polyphenolic antifungal agents. First, the proportion of biomass was obtained from the Arabica green bean processing. The yield of by-products was recorded, and the high-potency biomass was serially extracted with organic solvents for the polyphenol fraction. Quantification of the polyphenols was performed by High Performance Liquid Chromatography (HPLC), then further confirmed by mass spectrometry modes of the liquid chromatography–quadrupole time-of-flight (QTOF). Then, the fraction was used to test antifungal activities against Alternaria brassicicola, Pestalotiopsis sp. and Paramyrothecium breviseta. The results illustrated that caffeic acid and epigallocatechin gallate represented in the polyphenol fraction actively inhibited these fungi with an inhibitory concentration (IC50) of 0.09, 0.31 and 0.14, respectively. This study is also the first report on the alternative use of natural biocontrol agent of P. breviseta, the pathogen causing leaf spot in the Arabica coffee.

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“…The rates of efficacy were 43.88 % and 22.63 % for TCA (2%) and TCA (1%) respectively (Figure 3b). Previously, different microorganism such as antagonistic fungi [24], endophytic Streptomyces bacteria [25], [26] or their crude extracts such as marine algae extracts [27], and medicinal plants extracts [4] were tested to control A. brassicicola. This study is the first to evaluate the antifungal activity of X. szentirmaii CFS and TCA against A. brassicicola.…”

Section: Resultsmentioning

confidence: 99%

“…The causing agent of Black Spot Disease, Alternaria brassicicola, is an important plant pathogenic fungus which can affectvarious plants including spinach [1], radish [2], cauliflower, celery cabbage, broccoli, [2], [3] and Chinese kale [4], [5]. The pathogen attacks plant's leaves and stems causing brown spots on leaves which can be yellow halos at an early stage.…”

Section: Introductionmentioning

confidence: 99%

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Alternaria brassicicola’ya karşı Trans-cinnamic Asit ve Xenorhabdus szentirmaii’nin Antifungal Metabolitlerinin Kullanım Potansiyellerinin Araştırılması

GÜLCÜ,

ALTIN

2024

Düzce Üniversitesi Bilim Ve Teknoloji Dergisi

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In the recent study, antifungal activity of trans-cinamic acid (TCA) and cell-free supernatant (CFS) of X. szentirmaii were evaluated against Alternaria brassicola that cause Black spot disease. The results showed that TCA is more suppressive than CFS of X. szentirmaii in the control of A. brassicola. In petri assays, the highest suppressive effect on spore germination was in TCA treatments compared to CFS of X. szentirmaii. Moreover, no germ tube elongation was observed in all tested concentrations (0.25%, 0.50%, 1%, 2%) of TCA. The highest level of mycelial growth inhibition was exhibited by TCA (2%) followed by TCA (1%). The curative and protective activity of TCA on disease severity of A. brassicola were also evaluated on broccoli plant. In the curative activity assays, disease severity was 56.33% and 54.03% for TCA (1%) and TCA (2%) respectively. Whereas in the protective activity, TCA (2%) suppressed the disease severity significantly greater than TCA (1%). The disease severity of A. brassicola after TCA application on broccoli plants was 25.21% and 17.37% for TCA (1%) and TCA (2%) respectively. Similar data were obtained in the efficacy of TCA on A. brassicola. In the curative activity assays, TCA (1%) and TCA (2%) exhibited the efficacy with a rate of 20,57% and 23,02% respectively. However, TCA (2%) showed significantly higher efficacy than TCA (1%) in the protective activity. The current study provides that TCA has a great potential to suppress A. brassicola and can be a good alternative instead of synthetic fungicides.

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Fungicidal activity of Thai medicinal plant extracts against Alternaria brassicicola causing black spot of Chinese kale

Cited by 15 publications

References 32 publications

The Antifungal Activity of Gallic Acid and Its Derivatives against Alternaria solani, the Causal Agent of Tomato Early Blight

The Antifungal Activity of Gallic Acid and Its Derivatives against Alternaria solani, the Causal Agent of Tomato Early Blight

Recovery of Polyphenolic Fraction from Arabica Coffee Pulp and Its Antifungal Applications

Alternaria brassicicola’ya karşı Trans-cinnamic Asit ve Xenorhabdus szentirmaii’nin Antifungal Metabolitlerinin Kullanım Potansiyellerinin Araştırılması

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