Alternaria brassicicola’ya karşı Trans-cinnamic Asit ve Xenorhabdus szentirmaii’nin Antifungal Metabolitlerinin Kullanım Potansiyellerinin Araştırılması

Abstract

In the recent study, antifungal activity of trans-cinamic acid (TCA) and cell-free supernatant (CFS) of X. szentirmaii were evaluated against Alternaria brassicola that cause Black spot disease. The results showed that TCA is more suppressive than CFS of X. szentirmaii in the control of A. brassicola. In petri assays, the highest suppressive effect on spore germination was in TCA treatments compared to CFS of X. szentirmaii. Moreover, no germ tube elongation was observed in all tested concentrations (0.25%, 0.50%, 1%, 2%) of TCA. The highest level of mycelial growth inhibition was exhibited by TCA (2%) followed by TCA (1%). The curative and protective activity of TCA on disease severity of A. brassicola were also evaluated on broccoli plant. In the curative activity assays, disease severity was 56.33% and 54.03% for TCA (1%) and TCA (2%) respectively. Whereas in the protective activity, TCA (2%) suppressed the disease severity significantly greater than TCA (1%). The disease severity of A. brassicola after TCA application on broccoli plants was 25.21% and 17.37% for TCA (1%) and TCA (2%) respectively. Similar data were obtained in the efficacy of TCA on A. brassicola. In the curative activity assays, TCA (1%) and TCA (2%) exhibited the efficacy with a rate of 20,57% and 23,02% respectively. However, TCA (2%) showed significantly higher efficacy than TCA (1%) in the protective activity. The current study provides that TCA has a great potential to suppress A. brassicola and can be a good alternative instead of synthetic fungicides.