Fungi fabrication, characterization, and anticancer activity of silver nanoparticles using metals resistant Aspergillus niger

Lan, Nguyễn Thúy; Veeraragavan, Geetha Royapuram; Brindhadevi, Kathirvel; Chinnathambi, Arunachalam; Salmen, Saleh H.; Alharbi, Sulaiman Ali; Ramakrishnan, K. K.

doi:10.1016/j.envres.2022.112721

Cited by 16 publications

(10 citation statements)

References 49 publications

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“…After exposing MCF-7 cells (human breast adenocarcinoma) to the nanoparticles, a low IC50 value (121.23 µg/cm3) was obtained, indicating high cytotoxicity and the potential for tumor control [51]. Fungi-made silver nanoparticles exhibited strong anticancer activity against many tumor cells lines, with IC 50 values consistent with those previously reported [37,39,[52][53][54][55].…”

Section: Discussionsupporting

confidence: 80%

Creating silver nanoparticles through the biogenic synthesis process using marine fungi Aspergillus flavus OQ651270: Examining their characterization and conducting biological studies.

Al-Agamy,

Kelany,

Alhuzani

et al. 2023

Preprint

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Silver nanoparticles (Ag-NPs) have gained widespread attention due to their diverse applications in modern technology, prompting extensive research and development efforts. This study focuses on investigating the antibacterial properties of Ag-NPs, encompassing their production, characterization, and biological aspects. The eco-friendly extracellular biosynthetic method employed in this work utilized extracts from the marine fungus Aspergillus flavus MK4 as reducing agents for nanoparticle synthesis. UV-visible spectroscopy revealed the colloidal Ag-NPs, with a Plasmon absorbance peak observed at 450 nm. Transmission electron microscopy (TEM) elucidated the spherical shape and 15 nm size of the Ag-NPs. Evaluation against both Gram-negative and Gram-positive bacteria demonstrated significant antibacterial activity. Cytotoxicity against the HepG-2 cell line was assessed using the MTT assay, and antioxidant properties were examined through the radical scavenging (DPPH) assay. The synthesized Ag-NPs exhibit promising characteristics for applications in antibacterial, anticancer, and wound-healing contexts. Aspergillus flavus MK4, with its robust Ag-NP synthesis capability, produces well-characterized nanoparticles with proven antibacterial, antioxidant, anticancer, and wound-healing properties.

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Section: Discussionsupporting

confidence: 80%

Creating silver nanoparticles through the biogenic synthesis process using marine fungi Aspergillus flavus OQ651270: Examining their characterization and conducting biological studies.

Al-Agamy,

Kelany,

Alhuzani

et al. 2023

Preprint

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show abstract

“…The analysis of the FTIR spectra of the AgNPs 35 (Figure 4a) and AgNPs 58 (Figure 4b) showed different bands indicating the presence of biomolecules with functional groups such as amines (NH and CN), alcohols (OH) and alkanes (C═C, CH). These groups are probably involved in the reduction and coating of the NPs, acting in their stabilization preventing agglomeration and aggregation [27].…”

Section: Resultsmentioning

confidence: 99%

Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens

et al. 2022

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To find effective silver nanoparticles (AgNPs) for control of phytopathogens, in this study, two strains of actinomycetes isolated from the soil of the Brazilian biome Caatinga (Caat5–35) and from mangrove sediment (Canv1–58) were utilized. The strains were identified by using the 16S rRNA gene sequencing as Streptomyces sp., related to Streptomyces mimosus species. The obtained AgNPs were coded as AgNPs 35 and AgNPs58 and characterized by size and morphology using dynamic light scattering, zeta potential, transmission electron microscopy, and Fourier transformed infrared (FTIR). The antifungal activity of the AgNPs35 and AgNPs58 was evaluated in vitro by the minimal inhibitory concentration (MIC) assay on the phytopathogens, Alternaria solani, Alternaria alternata, and Colletotrichum gloeosporioides. The phytotoxic effect was evaluated by the germination rate and seedling growth of rice (Oryza sativa). AgNPs35 and AgNPs58 showed surface plasmon resonance and average sizes of 30 and 60 nm, respectively. Both AgNPs presented spherical shape and the FTIR analysis confirmed the presence of functional groups such as free amines and hydroxyls of biomolecules bounded to the external layer of the nanoparticles. Both AgNPs inhibited the growth of the three phytopathogens tested, and A. alternate was the most sensible (MIC ≤ 4 µM). Moreover, the AgNPs35 and AgNPs58 did not induce phytotoxic effects on the germination and development of rice seedlings. In conclusion, these AgNPs are promising candidates to biocontrol of these phytopathogens without endangering rice plants.

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“…The diameters of the nanoparticles of this study were varied from 35 to 80 nm, with an average size of 53 nm. The synthesized BT-AgNPs size varied from 20.15 to 22.21 nm, as demonstrated by Chi et al [ 95 ]. Furthermore, the average size of the AgNPs produced by the extract of walnut fruit ( Juglans regia ) was 31.4 nm [ 96 ].…”

Section: Discussionmentioning

confidence: 85%

Unravelling the Antimicrobial, Antibiofilm, Suppressing Fibronectin Binding Protein A (fnba) and cna Virulence Genes, Anti-Inflammatory and Antioxidant Potential of Biosynthesized Solanum lycopersicum Silver Nanoparticles

Mekky,

Abdelaziz,

Youssef

et al. 2024

Medicina

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Background and Objectives: Urinary tract infections [UTIs] are considered the third most known risk of infection in human health around the world. There is increasing appreciation for the pathogenicity of Gram-positive and Gram-negative strains in UTIs, aside from fungal infection, as they have numerous virulence factors. Materials and Methods: In this study, fifty urine samples were collected from patients suffering from UTI. Among the isolates of UTI microbes, six isolates were described as MDR isolates after an antibiotic susceptibility test carried out using ten different antibiotics. An alternative treatment for microbial elimination involved the use of biosynthesized silver nanoparticles (AgNPs) derived from Solanum lycopersicum [S. cumin]. Results: The sizes and shapes of AgNPs were characterized through TEM imaging, which showed spherical particles in a size range of 35–80 nm, of which the average size was 53 nm. Additionally, the silver nanoparticles (AgNPs) demonstrated inhibitory activity against Staphylococcus aureus (OR648079), exhibiting a 31 mm zone of inhibition at a minimum inhibitory concentration (MIC) of 4 mg/mL and a minimum bactericidal concentration (MBC) of 8 mg/mL. This was followed by Aspergillus niger (OR648075), which showed a 30 mm inhibition zone at an MIC of 16 mg/mL and a minimum fungicidal concentration (MFC) of 32 mg/mL. Then, Enterococcus faecalis (OR648078), Klebsiella pneumoniae (OR648081), and Acinetobacter baumannii (OR648080) each displayed a 29 mm zone of inhibition at an MIC of 8 mg/mL and an MBC of 16 mg/mL. The least inhibition was observed against Candida auris (OR648076), with a 25 mm inhibition zone at an MIC of 16 mg/mL and an MFC of 32 mg/mL. Furthermore, AgNPs at different concentrations removed DPPH and H2O2 at an IC50 value of 13.54 μg/mL. Also, AgNPs at 3 mg/mL showed remarkable DNA fragmentation in all bacterial strains except Enterococcus faecalis. The phytochemical analysis showed the presence of different active organic components in the plant extract, which concluded that rutin was 88.3 mg/g, garlic acid was 70.4 mg/g, and tannic acid was 23.7 mg/g. Finally, AgNPs concentrations in the range of 3–6 mg/mL showed decreased expression of two of the fundamental genes necessary for biofilm formation within Staphylococcus aureus, fnbA (6 folds), and Cna (12.5 folds) when compared with the RecA gene, which decreased by one-fold when compared with the control sample. These two genes were submitted with NCBI accession numbers [OR682119] and [OR682118], respectively. Conclusions: The findings from this study indicate that biosynthesized AgNPs from Solanum lycopersicum exhibit promising antimicrobial and antioxidant properties against UTI pathogens, including strains resistant to multiple antibiotics. This suggests their potential as an effective alternative treatment for UTIs. Further research is warranted to fully understand the mechanisms of action and to explore the therapeutic applications of these nanoparticles in combating UTIs.

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Fungi fabrication, characterization, and anticancer activity of silver nanoparticles using metals resistant Aspergillus niger

Cited by 16 publications

References 49 publications

Creating silver nanoparticles through the biogenic synthesis process using marine fungi Aspergillus flavus OQ651270: Examining their characterization and conducting biological studies.

Creating silver nanoparticles through the biogenic synthesis process using marine fungi Aspergillus flavus OQ651270: Examining their characterization and conducting biological studies.

Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens

Unravelling the Antimicrobial, Antibiofilm, Suppressing Fibronectin Binding Protein A (fnba) and cna Virulence Genes, Anti-Inflammatory and Antioxidant Potential of Biosynthesized Solanum lycopersicum Silver Nanoparticles

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