Creating silver nanoparticles through the biogenic synthesis process using marine fungi Aspergillus flavus OQ651270: Examining their characterization and conducting biological studies.

Abstract

Silver nanoparticles (Ag-NPs) have gained widespread attention due to their diverse applications in modern technology, prompting extensive research and development efforts. This study focuses on investigating the antibacterial properties of Ag-NPs, encompassing their production, characterization, and biological aspects. The eco-friendly extracellular biosynthetic method employed in this work utilized extracts from the marine fungus Aspergillus flavus MK4 as reducing agents for nanoparticle synthesis. UV-visible spectroscopy revealed the colloidal Ag-NPs, with a Plasmon absorbance peak observed at 450 nm. Transmission electron microscopy (TEM) elucidated the spherical shape and 15 nm size of the Ag-NPs. Evaluation against both Gram-negative and Gram-positive bacteria demonstrated significant antibacterial activity. Cytotoxicity against the HepG-2 cell line was assessed using the MTT assay, and antioxidant properties were examined through the radical scavenging (DPPH) assay. The synthesized Ag-NPs exhibit promising characteristics for applications in antibacterial, anticancer, and wound-healing contexts. Aspergillus flavus MK4, with its robust Ag-NP synthesis capability, produces well-characterized nanoparticles with proven antibacterial, antioxidant, anticancer, and wound-healing properties.