Fungal Musculoskeletal Infections

Henry, Michael; Miller, Andy O.; Walsh, Thomas J.; Brause, Barry D.

doi:10.1016/j.idc.2017.01.006

Cited by 47 publications

(27 citation statements)

References 85 publications

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“…Approximately, 300 fungi are known to be pathogenic to humans 71 . For clinical laboratories to diagnose, the growth of fungi occurs on enriched bacteriologic media 72 . However, numerous fungal cultures need days to weeks of incubation for first growth and the proliferation of bacteria present in the specimen is more rapid 73 .…”

Section: Crispr/cas–based In Vitro Diagnosticsmentioning

confidence: 99%

CRISPR‐Cas system for biomedical diagnostic platforms

Wang

Cui

2020

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Clustered short palindrome repeats with regular intervals, abbreviated as CRISPR, and functions as a self‐defense system for prokaryotes, detecting particular pathogenic nucleic acid, interfering with the functions of exoteric DNA, and protecting them against foreign invaders. In recent years, CRISPR has attracted increasing interests in the in vitro diagnostic field because of its inherent allele specificity, which is one of the critical factors for the successful application of this technology in the development of high‐precision treatment and diagnosis. Herein, this review article aims to provide an overview of CRISPR‐CRISPR associated proteins (Cas) based biomedical diagnostics, including the biological mechanism, biomaterials, and applications. This paper first briefly introduces the development history and biological characteristics of the CRISPR‐Cas system, and then summarizes the application status and development trend of the CRISPR‐Cas system in the detection and identification of particular pathogens, specifically displaying a brilliant prospect in the most recent outbreak of novel coronavirus (formerly named 2019‐nCoV). Moreover, its potential diagnostic power in oncogene mutations and single nucleotide variations detecting are assembled. Finally, we discuss challenges and future prospects of CRISPR‐Cas system based diagnostic platforms in biomedicine, hoping to further inspire the development of biomedical diagnostics.

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Section: Crispr/cas–based In Vitro Diagnosticsmentioning

confidence: 99%

CRISPR‐Cas system for biomedical diagnostic platforms

Wang

Cui

2020

VIEW

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“…Fortunately, mycologic investigation was performed in his first visit to our hospital and C. parapsilosis was identified in the joint fluid and synovial tissue. Previously, many lines of evidence have indicated that most patients with fungal infection are immunosuppressed with predisposing factors, including systemic disease, recent surgery, chemotherapy, long-term antibiotics use, corticosteroid therapy and insertion of central venous catheters [5, 6]. In the current case, the patient had poorly controlled blood glucose and several events of corticosteroid injection of his knee which might cause global and local impairment of immunity.…”

Section: Discussionmentioning

confidence: 77%

Recurrent arthritis caused by Candida parapsilosis: a case report and literature review

et al. 2019

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Background Candida arthritis is extremely rare and also represents a major challenge of diagnosis and treatment. Here we reported a rare case of recurrent arthritis caused by Candida parapsilosis. Case presentation A 56-year-old Chinese male suffered from recurrent pain and swelling in his right knee after several times of “small needle-knife” acupuncture and corticosteroid injection of the joint. Candida parapsilosis was cultured in his synovial fluid and identified by sequencing of its Internal Transcribed Spacer (ITS) gene. Here we present the radiological characteristics, arthroscopic pictures, and synovium pathology of this patient. Also, blood test and chemical analysis of his synovial fluid were listed as well as the ITS sequence of this Candida species identified. The patient underwent thorough arthroscopic debridement and then set on fluconazole 400 mg daily for 12 months. His symptoms resolved and no relapse was observed on the last follow-up. Additionally, a brief but comprehensive review of C. parapsilosis arthritis episodes from past to now were studied. Conclusion With the detailed clinical information reported in this case and our literature review, we hope they would add to our knowledge of C. parapsilosis arthritis - its clinical settings, laboratory features, radiological characteristics, arthroscopic findings and experience of management. Electronic supplementary material The online version of this article (10.1186/s12879-019-4255-1) contains supplementary material, which is available to authorized users.

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“…Aspergillosis is an opportunistic infection that mainly affects the respiratory tract and can be spread through blood contact [2]. Severe neutropenia, glucocorticoid use, transplantation and other immunosuppressive conditions may lead to invasive infections [3]. Aspergillus infection can occur in most organs, but the lung is the main target organ.…”

Section: Introductionmentioning

confidence: 99%

Aspergillus spondylitis: case series and literature review

Dai

Wang

Yin

et al. 2020

BMC Musculoskelet Disord

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Background: Spinal fungal infections, especially spinal Aspergillus infections, are rare in the clinic. Here, we introduce the clinical features, diagnosis, treatment, and prognoses of 6 cases of Aspergillus spondylitis. Methods: We retrospectively analysed the complete clinical data of patients with Aspergillus spondylitis treated in our hospital from January 2013 to January 2020. Results: Aspergillus fumigatus was isolated in 4 cases, and Aspergillus spp. and Aspergillus niger were isolated in 1 case each. All six patients reported varying degrees of focal spinal pain; one patient reported radiating pain, one patient experienced bowel dysfunction and numbness in both lower limbs, and three patients had fever symptoms. One case involved the thoracic spine, one case involved the thoracolumbar junction, and 4 cases involved the lumbar spine. Three patients were already in an immunosuppressed state, and three patients entered an immunosuppressed state after spinal surgery. All six patients were successfully cured, and five required surgery. Of the 5 patients who underwent surgical treatment, 2 had spinal cord compression symptoms, and 3 had spinal instability. At the end of follow-up, 1 patient reported left back pain and 1 patient reported left limb numbness. Conclusion: The clinical manifestations of Aspergillus spondylitis are non-specific, and the diagnosis depends on typical imaging findings and microbiological and histopathological examination results. When there is no spinal instability, spinal nerve compression symptoms, or progressive deterioration, antifungal therapy alone may be considered. If spinal instability, spinal nerve compression, or epidural abscess formation is present, surgery combined with antifungal therapy is recommended.

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Fungal Musculoskeletal Infections

Cited by 47 publications

References 85 publications

CRISPR‐Cas system for biomedical diagnostic platforms

CRISPR‐Cas system for biomedical diagnostic platforms

Recurrent arthritis caused by Candida parapsilosis: a case report and literature review

Aspergillus spondylitis: case series and literature review

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