Fungal laccases: Versatile tools for lignocellulose transformation

Piscitelli, Alessandra; Vecchio, Claudia Del; Faraco, Vincenza; Giardina, Paola; Macellaro, Gemma; Miele, Annalisa; Pezzella, Cinzia; Sannia, Giovanni

doi:10.1016/j.crvi.2011.06.007

Cited by 51 publications

(12 citation statements)

References 18 publications

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“…It was reported that the binding of NaN 3 to type II and type III copper sites affected internal electron transfer, thereby inhibiting laccase activity [ 32 ]. The anionic detergent SDS might cause a conformational change in LacA, leading to the inhibition of this enzyme [ 33 ]. However, Christian Johannes and Andrzej Majcherczyk] reported that the apparent inhibitory effects of L-cysteine and DTT were primarily caused by reduction of ABTS radical cation or non-enzymatic interactions with unreacted ABTS rather than by the inhibition of laccase [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

A novel homodimer laccase from Cerrena unicolor BBP6: Purification, characterization, and potential in dye decolorization and denim bleaching

et al. 2018

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The white-rot fungus Cerrena unicolor BBP6 produced up to 243.4 U mL-1 laccase. A novel laccase isoform LacA was purified; LacA is a homodimer with an apparent molecular mass of 55 kDa and an isoelectric point of 4.7. Its optimal pH was 2.5, 4.0, and 5.5 when 2, 2’-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS), guaiacol, and 2, 6-dimethoxyphenol (2, 6-DMP) were used as the substrates, respectively. The optimal temperature was 60°C for ABTS and 80°C for both guaiacol and 2, 6-DMP. LacA retained 82–92% activity when pH was greater than 4 and 42%-92% activity at or below 50°C. LacA was completely inhibited by 0.1 mM L-cysteine, 1 mM Dithiothreitol, and 10 mM metal ions, Ca2+, Mg2+ and Co2+. LacA had good affinity for ABTS, with a Km of 49.1 μM and a kcat of 3078.9 s-1. It decolorized synthetic dyes at 32.3–87.1%. In the presence of 1-hydroxybenzotriazole (HBT), LacA decolorized recalcitrant dyes such as Safranine (97.1%), Methylene Blue (98.9%), Azure Blue (96.6%) and simulated textile effluent (84.6%). With supplemented manganese peroxidase (MnP), Mn2+ and HBT, the purified LacA and BBP6 fermentation broth showed great potential in denim bleaching, with an up to 5-fold increase in reflectance values.

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Section: Resultsmentioning

confidence: 99%

A novel homodimer laccase from Cerrena unicolor BBP6: Purification, characterization, and potential in dye decolorization and denim bleaching

et al. 2018

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“…Laccase producing microorganisms are both fungi (Trametes versicolor, Tremetes trogii, Phlebia floridensis), and bacteria (Citrobacter spp., Straphylococcus saprophlticus, Bacillus subtilis). Lac enzyme plays an important role in lignin degradation and modification processes which increase the yield of both hydrolysis and fermentation process (Piscitelli et al, 2011;Fillat et al, 2017). Laccase is an important enzyme since it oxidizes both toxic and non-toxic substrates.…”

Section: Laccasesmentioning

confidence: 99%

Recent Trends in the Pretreatment of Lignocellulosic Biomass for Value-Added Products

et al. 2018

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Lignocellulosic biomass (LCB) is the most abundantly available bioresource amounting to about a global yield of up to 1. 3 billion tons per year. The hydrolysis of LCB results in the release of various reducing sugars which are highly valued in the production of biofuels such as bioethanol and biogas, various organic acids, phenols, and aldehydes. The majority of LCB is composed of biological polymers such as cellulose, hemicellulose, and lignin, which are strongly associated with each other by covalent and hydrogen bonds thus forming a highly recalcitrant structure. The presence of lignin renders the bio-polymeric structure highly resistant to solubilization thereby inhibiting the hydrolysis of cellulose and hemicellulose which presents a significant challenge for the isolation of the respective bio-polymeric components. This has led to extensive research in the development of various pretreatment techniques utilizing various physical, chemical, physicochemical, and biological approaches which are specifically tailored toward the source biomaterial and its application. The objective of this review is to discuss the various pretreatment strategies currently in use and provide an overview of their utilization for the isolation of high-value bio-polymeric components. The article further discusses the advantages and disadvantages of the various pretreatment methodologies as well as addresses the role of various key factors that are likely to have a significant impact on the pretreatment and digestibility of LCB.

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“…P. ostreatus belongs to a class of white-rot fungi that produces laccases, manganese peroxidases but not lignin peroxidases (Giardina et al 2000). Laccases are synthesized in multiple isoforms depending on the fungal species and the environmental conditions and this variety is related to the diversity of their roles: lignin degradation/synthesis, fruiting bodies development, pigment production, cell detoxification (Piscitelli et al 2011). The biochemical diversity of laccase isoenzymes appears to be due to the multiplicity of laccase genes; however, regulation of their expression can be substantially diverse between the fungal species (Palmieri et al 2003).…”

Section: Ligninolytic Enzymesmentioning

confidence: 99%

Pretreatment strategies for delignification of sugarcane bagasse: a review

Karp

Woiciechowski

Soccol

et al. 2013

Braz. arch. biol. technol.

145

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The valorization of agro-residues by biological routes is a key technology that contributes to the development of sustainable processes and the generation of value-added products. Sugarcane bagasse is an agro-residue generated by the sugar and alcohol industry in Brazil (186 million tons per year), composed essentially of cellulose (32-44%), hemicellulose (27-32%) and lignin (19-24%). The conversion of sugarcane bagasse into fermentable sugars requires essentially two steps: pretreatment and hydrolysis. The aim of the pretreatment is to separate the lignin and break the structure of lignocellulose, and it is one of the most critical steps in the process

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Fungal laccases: Versatile tools for lignocellulose transformation

Cited by 51 publications

References 18 publications

A novel homodimer laccase from Cerrena unicolor BBP6: Purification, characterization, and potential in dye decolorization and denim bleaching

A novel homodimer laccase from Cerrena unicolor BBP6: Purification, characterization, and potential in dye decolorization and denim bleaching

Recent Trends in the Pretreatment of Lignocellulosic Biomass for Value-Added Products

Pretreatment strategies for delignification of sugarcane bagasse: a review

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