“…However, because cellulosic components (i.e., substrates for ethanol production) are strongly covered with lignin in the lignocellulosic biomass, pretreatment (delignification) is necessary for efficient enzymatic saccharification followed by alcohol fermentation to increase the accessibility of cellulose to cellulases (Karp et al, 2013). Various pretreatment methods have been developed and applied to target the conversion of biomass to biofuel including: acid treatment (Torget et al, 1988), alkaline treatment (Ucar, 1990), ammonia fiber expansion (Dale et al, 1996), organosolv treatment (Bonn et al, 1987), liquid hot water treatment (Sreenath et al, 1999), microwave irradiation (Ooshima et al, 1984), superheated steam treatment (Bahrin et al, 2012), and steam explosion (Ramos et al, 1992).…”