Fungal growth on soft contact lenses: A SEM-study

Jongebloed, W.L.; Humalda, D.; Worst, J.F.G.

doi:10.1007/bf00142706

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…A few years ago, a SEM analysis allowed to observe globular deposits of fungal spores on soft CLs discarded because of irritation of the cornea. 30 The techniques were also used to evaluate the role of calcium in nodular deposits on the front surface of worn CLs 31 or to compare new and worn siloxane-hydrogel CLs in terms of microscopic structure, surface morphology, and loading of hyaluronan. 26,32 In very recent years, SEM and EDX were used to investigate the surfaces and principal elements of the colorants of cosmetically tinted CLs.…”

Section: Discussionmentioning

confidence: 99%

Interaction between siloxane-hydrogel contact lenses and eye cosmetics: Aluminum as a marker of adsorbed mascara deposits

Zeri

Borghesi

Acciarri

et al. 2020

Polymers and Polymer Composites

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The purpose of the study was to investigate the presence of mascara deposits on polymeric siloxane-hydrogel contact lenses (CLs) after in vivo 8 h of wear and to compare the affinity to mascara of two siloxane-hydrogels through in vitro contamination tests. The siloxane-hydrogel CLs were the Filcon V monthly replacement OPEN30 and the Delefilcon A daily disposable DAILIES TOTAL1 showing a water gradient from the core to the outermost hydrophilic surface layer. The investigated cosmetic was a blue mascara. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) were carried out on new CLs, CLs exposed in vitro to mascara, and CLs worn for 8 h by mascara wearers (after rinsing the CLs in sodium chloride solution). Images by an optical microscope were acquired and processed on new CLs and CLs treated in vitro. EDX analyses on mascara allowed to characterize its elemental composition including aluminum (Al), which is neither a tear nor a CL component, thus being a marker of the presence of mascara deposits on polymeric siloxane-hydrogels. Adsorbed deposits containing Al were observed by SEM/EDX on all worn CLs of both materials, which showed a special affinity for the mascara components, more marked than for the tear components. By processing the images taken by optical microscopy after in vitro tests, the presence of mascara deposits was found to be more than two times higher in Delefilcon A compared to Filcon V CLs.

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Section: Discussionmentioning

confidence: 99%

Interaction between siloxane-hydrogel contact lenses and eye cosmetics: Aluminum as a marker of adsorbed mascara deposits

Zeri

Borghesi

Acciarri

et al. 2020

Polymers and Polymer Composites

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“…In order to perform a more detailed analysis of the yeast adhesion and the deterioration of the surface of the lenses, two lenses of vifilcon A were randomly selected and observed, using a scanning electron microscope (SEM) [6,13,22]. Additionally, two more lenses of vifilcon A were analyzed with a confocal scanning laser microscope (CSLM), in order to observe the morphology of the inner colonies and to quantify their penetration into the matrix.…”

Section: Colonizationmentioning

confidence: 99%

“…Specifically, filamentous fungi such as Aspergillus, Penicillium, Fusarium, Alternaria, Acremonium, Cladosporium, Scopulariopsis, and Paecilomyces, can invade discarded contact lenses [4, 11-13, 16, 23, 25, 26]. Moreover, yeasts such as Candida, Rhodotorula, Torulopsis, and Cryptococcus have also been isolated from contact lenses, although the number of species that can colonize them is lower than that of filamentous fungi [12,13,15,16,19,[24][25][26]. Since filamentous fungi and yeasts can produce ocular infections, the colonization of contact lenses by fungi has been studied under an ophthalmological perspective [1,2,14,19,25,26].…”

Section: Introductionmentioning

confidence: 99%

Colonization of hydrophilic contact lenses by yeast

Marqués-Calvo

2004

J IND MICROBIOL BIOTECHNOL

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The growth of six strains of yeast was analyzed in vitro in order to assess their capacity for colonizing (adhesion and invasion) hydrophilic contact lenses. Lenses with different water content were cultured in two culture media for 3, 7, 14, and 21 days. Only strain 93150 of Candida albicans could adhere to and invade the polymers. Specifically, fungal growth was observed in cultures with Sabouraud's broth. The degree of yeast colonization of contact lenses was significantly related to the species, the strain, and the culture medium in which the yeast and lenses were cultured. The results here obtained were compared with those reported for the filamentous fungus Aspergillus niger 2700. For both microorganisms, the strain and the medium in which the lenses and microorganism were cultured influenced the colonization, but the percentage of colonized lenses, the degree of colonization, and the density and size of the internalized colonies were always noticeably lower for C. albicans 93150. Colonization by A. niger 2700 was also related to the type of material of the lenses and the incubation period. For both microorganisms, when the strain is right and the growth and development are correct, colonization of hydrophilic contact lenses occurs.

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Fungal Invasion of Seven Hydrophilic Contact Lenses

Kirsch

Brownstein

1993

American Journal of Ophthalmology

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Fungal growth on soft contact lenses: A SEM-study

Cited by 5 publications

References 11 publications

Interaction between siloxane-hydrogel contact lenses and eye cosmetics: Aluminum as a marker of adsorbed mascara deposits

Interaction between siloxane-hydrogel contact lenses and eye cosmetics: Aluminum as a marker of adsorbed mascara deposits

Colonization of hydrophilic contact lenses by yeast

Fungal Invasion of Seven Hydrophilic Contact Lenses

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