Fungal Endophytes in Roots of Aralia Species and Their Antifungal Activity

Paul, Narayan Chandra; Kim, Won Ki; Woo, Sung Kyoon; Park, Myung Soo; Yu, Seung Hun

doi:10.5423/ppj.2007.23.4.287

Cited by 39 publications

(35 citation statements)

References 24 publications

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“…1), conWrming that a few species are frequent colonizers, and yet a majority of the groups are rare inhibitors in woody plants in temperate regions and tropical regions [13,24]. Diaporthe, Phomopsis (anamorph of Diaporthe), Acremonium, and Pezicula are frequent colonizers in T. chinensis, whereas they are not cosmopolitan species within other Taxus plants, such as Taxus mairei and Taxus baccata [3,21], showing dominant genera residing in diVerent yews are distinct.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of endophytic taxol-producing fungi from Taxus chinensis

Kaihui

Ding

Deng

et al. 2009

J Ind Microbiol Biotechnol

156

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This study investigated the endophytic fungi diversity of Taxus chinensis and screened the taxol-producing fungi in the host. A total of 115 endophytic fungi isolates obtained from bark segments of T. chinensis were grouped into 23 genera based on the morphological traits and sequence analysis of the internal transcribed spacers (ITS1-5.8S-ITS2), indicating endophytic fungi in T. chinensis are diverse and abundant. Diaporthe, Phomopsis (anamorph of Diaporthe), Acremonium, and Pezicula were the dominant genera, whereas the remaining genera were infrequent groups. The 13 representative species of the distinct genera were capable of producing taxol verified by reverse-phase high performance liquid chromatography (HPLC). Among the taxol-producing fungi, the yield of taxol produced by the Metarhizium anisopliae, H-27 was 846.1 microg l(-1) in reformative potato dextrose liquid medium, and the fungal taxol was further validated by mass spectrometry (MS). The taxol-producing fungi (92.3%) were infrequent communities, suggesting that infrequent fungi associated with T. chinensis might be a fascinating reservoir of taxol-generating fungi.

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of endophytic taxol-producing fungi from Taxus chinensis

Kaihui

Ding

Deng

et al. 2009

J Ind Microbiol Biotechnol

156

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“…Endophytic microorganism have been found in all plant families (Sieber et al, 1988), which represent many species in different climate regions of the world (Bussaban et al, 2001;Fisher et al, 1992;Larran et al, 2001Larran et al, , 2002Paul et al, 2007;Petrini and Carroll, 1981;Spurr and Welty, 1975). Endophytic attention has increased in recent years because of its taxonomic diversity (Chen et al, 2011a), its multiple functions including potential use as genetic vectors (Murray et al, 1992); host plant growth promotion and fitness (Baltruschat et al, 2008;Tanaka et al, 2006;Tudzynski and Sharon, 2002).…”

mentioning

confidence: 99%

“…Endophytic attention has increased in recent years because of its taxonomic diversity (Chen et al, 2011a), its multiple functions including potential use as genetic vectors (Murray et al, 1992); host plant growth promotion and fitness (Baltruschat et al, 2008;Tanaka et al, 2006;Tudzynski and Sharon, 2002). Furthermore, they are the source of secondary metabolites (Fisher et al, 1986;Paul et al, 2007;Strobel et al, 1996) and biological control agents (Bacon, 1990;Clay, 1989;Dorworth and Callan, 1996;Schardl et al, 1991). Carroll (1988) has demonstrated the enhancing of the host survival against fungal pathogens in some plant-endophyte associations.…”

mentioning

confidence: 99%

Distribution and Antifungal Activity of Endophytic Fungi in Different Growth Stages of Chili Pepper (Capsicum annuum L.) in Korea

Paul¹,

Deng²,

Sang³

et al. 2012

The Plant Pathology Journal

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This study investigated the distribution of endophytic fungi obtained from the surface sterilized tissues of leaves, stems and roots of chili pepper (Capsicum annuum L.) plants in seedling, flowering and fruiting stages in Korea and their antifungal activity. A total of 481 isolates were recovered and were identified using molecular techniques. Based on rDNA ITS gene sequence and phylogenetic analysis, 21 fungal genera were characterized, belonging to 16 Ascomycota and 5 Basidiomycota. Penicillium in seedling stage, Fusarium in flowering stage, Colletotrichum followed by Fusarium, Alternaria and Xylaria in fruiting stage was predominant and Alternaria, Cladosporium and Fusarium were common in all growth stages. Among 481 endophytes 90 phenotypes were evaluated for the antimicrobial activity against three major pathogens (Phytophthora capsici, Colletotrichum acutatum and Fusarium oxysporum) of chili pepper. Among them 16 isolates inhibited the growth of at least one test microorganisms. Three strains showed a broad spectrum antifungal activity and displayed strong inhibition against chili pepper pathogenic fungi.

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“…DNA was extracted from fungal mycelia by the CTAB method (Paul et al 2007). A pair of universal primers ITS1 (5 0 -TCC GTA GGT GAA CCT GCG G-3 0 ) and ITS4 (5 0 -TCC TCC GCT TAT TGA TAT GC-3 0 ) was used to amplify the target genes.…”

Section: Taxonomic Identification Of Fungal Isolatesmentioning

confidence: 99%

Endophytic fungi associated withTaxus fuana(West Himalayan Yew) of Pakistan: potential bio-resources for cancer chemopreventive agents

Fatima

Kondratyuk

Park

et al. 2016

Pharmaceutical Biology

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Context: Endophytic fungi, being a prolific source of bioactive secondary metabolites, are of great interest for natural product discovery. Objective: Isolation and partial characterization of endophytic fungi inhabiting the leaves and woody parts of Taxus fuana Nan Li & R.R. Mill. (Taxaceae) and evaluation of biological activity. Materials and methods: Endophytic fungal isolates were identified by molecular analysis of internal transcribed spacer (ITS) regions of 18S rDNA. Extracts of the endophytic fungi cultured on potato dextrose agar and modified medium were evaluated using cancer chemoprevention bioassays [inhibition of TNF-ainduced NFjB, aromatase and inducible nitric oxide synthase (iNOS); induction of quinone reductase 1 (QR1)] and growth inhibition with MCF-7 cells. Results: Nine of 15 fungal isolates were identified as belonging to Epicoccum, Mucor, Penicillium, Chaetomium, Paraconiothriym, Plectania or Trichoderma. Five of the 15 extracts inhibited NFjB activity (IC 50 values ranging between 0.18 and 17 lg/mL) and five inhibited iNOS (IC 50 values ranging between 0.32 and 12.9 lg/mL). In the aromatase assay, only two isolates mediated inhibition (IC 50 values 12.2 and 10.5 lg/ mL). With QR1 induction, three extracts exhibited significant activity (concentrations to double activity values ranging between 0.20 and 5.5 lg/mL), and five extracts inhibited the growth of MCF-7 cells (IC 50 values ranging from 0.56 to 17.5 lg/mL). Six active cultures were derived from woody parts of the plant material. Conclusion: The endophytic fungi studied are capable of producing pharmacologically active natural compounds. In particular, isolates derived from the wood of Taxus fuana should be prioritized for the isolation and characterization of bioactive constituents. ARTICLE HISTORY

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Fungal Endophytes in Roots of Aralia Species and Their Antifungal Activity

Cited by 39 publications

References 24 publications

Isolation and characterization of endophytic taxol-producing fungi from Taxus chinensis

Isolation and characterization of endophytic taxol-producing fungi from Taxus chinensis

Distribution and Antifungal Activity of Endophytic Fungi in Different Growth Stages of Chili Pepper (Capsicum annuum L.) in Korea

Endophytic fungi associated withTaxus fuana(West Himalayan Yew) of Pakistan: potential bio-resources for cancer chemopreventive agents

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