Fungal endophytes from higher plants: a prolific source of phytochemicals and other bioactive natural products

Aly, Amal H.; Debbab, Abdessamad; Kjer, Julia; Proksch, Peter

doi:10.1007/s13225-010-0034-4

Cited by 548 publications

(314 citation statements)

References 86 publications

Supporting

Mentioning

271

Contrasting

Unclassified

Order By: Relevance

“…rhynchopetala on the south coast of China. The mangrove-derived endophytic fungi are a promising source of diverse and structurally unprecedented bioactive natural compounds, which attract considerable attention (Sridhar 2004; Aly et al 2010; Debbab et al 2013). …”

Section: Introductionmentioning

confidence: 99%

Community structure of endophytic fungi of four mangrove species in Southern China

Sun

Chen

et al. 2016

Mycology

View full text Add to dashboard Cite

Mangrove forests play an important role in subtropical and tropical coastal ecosystems. Endophytic fungi are widely distributed in various ecosystems and have great contribution to global biodiversity. In order to better understand the effects of mangrove species and tissue types on endophytic fungal community, we investigated cultivable endophytic fungi in leaves and twigs of four mangroves Aegiceras corniculatum, Avicennia marina, Bruguiera gymnorrhiza, and Kandelia candel in Guangxi, China. The four tree species had similar overall colonisation rates of endophytic fungi (24–33%). The colonisation rates of endophytic fungi were higher in twigs (30–58%) than in leaves (6–25%) in the four plant species. A total of 36 endophytic fungal taxa were identified based on morphological characteristics and molecular data, including 35 Ascomycota and 1 Basidiomycota, dominated by Phomopsis, Phyllosticta, Xylaria, Leptosphaerulina, and Pestalotiopsis. The diversity of endophytic fungi was higher in twigs than in leaves in the four plant species. Some endophytic fungi showed host and tissue preference. The endophytic fungal community composition was different among four mangrove species and between leaf and twig tissues.

show abstract

Section: Introductionmentioning

confidence: 99%

Community structure of endophytic fungi of four mangrove species in Southern China

Sun

Chen

et al. 2016

Mycology

View full text Add to dashboard Cite

show abstract

“…Endophytes resided in the internal tissues of living plants occur in almost every plant on earth from the arctic to the tropics, and they are rich sources for bioactive natural products (Guo et al 2008;Aly et al 2010Aly et al , 2011Qin et al 2011). It is generally recognized that endophytes represent an important and largely untapped reservoir of unique chemical structures that have been modified through evolution (Gunatilaka 2006;Kharwar et al 2011) and exhibit the capability to produce the same functional compounds as their hosts, some examples include taxol (Stierle et al 1993(Stierle et al , 1995, podophyllotoxin , hypericin (Kusari et al 2008), and azadirachtin (Findlay et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production

Xiang

Xing

Chen

et al. 2013

Appl Microbiol Biotechnol

151

View full text Add to dashboard Cite

Camptothecin (CPT), the third largest anticancer drug, is produced mainly by Camptotheca acuminata and Nothapodytes foetida. CPT itself is the starting material for clinical CPT-type drugs, but the plant-derived CPT cannot support the heavy demand from the global market. Research efforts have been made to identify novel sources for CPT. In this study, three CPT-producing endophytic fungi, Aspergillus sp. LY341, Aspergillus sp. LY355, and Trichoderma atroviride LY357, were isolated and identified from C. acuminata. Most CPT produced by these fungi was found in the fermentation broth, and their corresponding CPT yields were 7. 93, 42.92, and 197.82 μg l −1 , respectively. The CPT-producing capability of LY341 and LY355 was completely lost after repeat subculturing. A substantial decrease of CPT production was also observed in the second generation of LY357. However, a stable and sustainable production of CPT was found from the second generation through the eighth generation of LY357. The fermentation medium, time, pH, temperature, and agitation rate were optimized for CPT production. Methyl jasmonate and XAD16 were proven to be an optimum elicitor and adsorbent resin, respectively, in view of that CPT yield was increased 3.4-and 11-fold through their use. A 50-to 75-fold increase of CPT yield was obtained when the optimized fermentation conditions, elicitor, and adsorbent resin were combined and applied to the culture of the seventh and eighth generations of LY357, and the highest CPT yield was 142.15 μg l −1 . The CPTproducing T. atroviride LY357 paves a potential to uncover the mysteries of CPT biosynthesis.

show abstract

“…The results of this present analysis provide physical evidence that the apparent inability of endophytic fungi to produce lovastatin secondary metabolite [13] is due solely to the lack of DNA sequences that are identical or homologous to lovastatin gene present in lovastatin producing organism. The lack of production of lovastatin by endophytic fungi is apparently not due to suboptimal physiological conditions or altered regulation of lovastatin biosynthetic pathway enzymes.…”

Section: Resultsmentioning

confidence: 65%

“…However, an endophytic strain of the same organism does not possess DNA sequences homologous to lovastatin genes. Considering the endophytic relationship with host plant systems, it may be an advantage for a fungal strain not to produce secondary metabolites that may interfere with plant growth promoting characteristics [13]. Thus, there probably was no need for retaining/acquiring lovastatin biosynthetic pathway genes by these endophytic fungi isolated from medicinal plants.…”

Section: Resultsmentioning

confidence: 99%

Bioinformatic Comparative Analysis of Lovastatin Gene Cluster in Endophytic Fungi and a Soil Fungus, Aspergillus terreus

Janakiraman¹

2014

MOJPB

View full text Add to dashboard Cite

Genes responsible for lovastatin production in certain fungi are often clustered (64 kb DNA). In the present study, we report the comparative analysis of the genome of sixty one endophytic fungi and a soil fungus for the presence of lovastatin gene sequences. Nucleotide sequences were obtained from NCBI and aligned with lovastatin gene cluster sequence (AF141924.1& AF141925.1). DNA sequences that are identical to lovastatin gene were identified in Aspergillus terreus, (AH007774.1), a soil isolate, whereas all the other sixty one endophytic fungi including a species of Aspergillus terreus showed very limited or no homology with the lovastatin gene cluster.

show abstract

Fungal endophytes from higher plants: a prolific source of phytochemicals and other bioactive natural products

Cited by 548 publications

References 86 publications

Community structure of endophytic fungi of four mangrove species in Southern China

Community structure of endophytic fungi of four mangrove species in Southern China

Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production

Bioinformatic Comparative Analysis of Lovastatin Gene Cluster in Endophytic Fungi and a Soil Fungus, Aspergillus terreus

Contact Info

Product

Resources

About