Functions of alpha 2 macroglobulins in pregnancy

Tayade, Chandrakant; Esadeg, Souad; Yuan, Fang; Croy, B. Anne

doi:10.1016/j.mce.2005.10.004

Cited by 59 publications

(45 citation statements)

References 46 publications

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“…[43][44][45] Another member of A2M family is the pregnancy zone protein (PZP) that we found express exclusively in Muse cells and not in bmMSCs or aMSCs (Suppl. File 5).…”

Section: Muse Cells Secrete Factors That May Play a Role In Stem Cellmentioning

confidence: 99%

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The secretome of MUSE cells contains factors that may play a role in regulation of stemness, apoptosis and immunomodulation

et al. 2016

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Mesenchymal stromal cells (MSCs) are a heterogeneous population, which contain several cell phenotypes: mesenchymal stem cells, progenitor cells, fibroblasts and other type of cells. Previously, we identified unique stem cells that we named multilineage-differentiating stress enduring (Muse) cells as one to several percent of MSCs of the bone marrow, adipose tissue and dermis. Among different cell populations in MSCs, Muse cells, positive for pluripotent surface marker SSEA-3, may represent cells responsible for pluripotent-like property of MSCs, since they express pluripotency genes, able to differentiated into triploblastic cells from a single cells and are self-renewable.MSCs release biologically active factors that have profound effects on local cellular dynamics. A thorough examination of MSC secretome seems essential for understanding the physiological functions exerted by these cells in our organism and also for rational cellular therapy design. In this setting, studies on secretome of Muse cells may shed light on pathways that are associated with their specific features.Our findings evidenced that secretomes of MSCs and Muse cells contain factors that regulate extracellular matrix remodeling, ox-redox activities and immune system. Muse cells appear to secrete factors that may preserve their stem cell features, allow survival under stress conditions and may contribute to their immunomodulation capacity.In detail, the proteins belonging to protein kinase A signaling, FXR/RXR activation and LXR/RXR activation pathways may play a role in regulation of Muse stem cell features. These last 2 pathways together with proteins associated with antigen presentation pathway and coagulation system may play a role in immunomodulation.

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“…[43][44][45] Another member of A2M family is the pregnancy zone protein (PZP) that we found express exclusively in Muse cells and not in bmMSCs or aMSCs (Suppl. File 5).…”

Section: Muse Cells Secrete Factors That May Play a Role In Stem Cellmentioning

confidence: 99%

“…This protein is fundamental for a successful pregnancy, since it avoids that maternal immune system attacks the allogeneic fetus. 45,46 The presence of A2M and PZP in the secretome of Muse cells may indicate that these cells may have significant immunomodulator capacity.…”

Section: Muse Cells Secrete Factors That May Play a Role In Stem Cellmentioning

confidence: 99%

The secretome of MUSE cells contains factors that may play a role in regulation of stemness, apoptosis and immunomodulation

et al. 2016

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“…Nonetheless, some possible candidates were anticipated to be immunoglobulin (18−19 kDa) 12) , transferrin (75 kDa) 13) , α2-macroglobulin (720 kDa for tetramer) 14) , and fibrinogen (340 kDa) 15) . Chromatograms of pure bovine serum albumin and lysozyme in Fig.…”

Section: Discussionmentioning

confidence: 99%

Chromatography of carbon nanotubes separated albumin from other serum proteins: a method for direct analysis of their interactions

et al. 2010

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Chromatography technology was employed to clarify the mechanism of interaction between multi-wall carbon nanotubes (MWCNT) and proteins. A column (16×100 mm) was packed with purified MWCNT, and various proteins were eluted with phosphate buffered saline (PBS) with and without gradient systems. It was found that albumin in bovine serum was eluted immediately from the column without any adsorption to MWCNT. Conversely, the non-albumin proteins, including a protein of 85 kDa molecular mass and a group of proteins with molecular masses higher than 115 kDa, exhibited considerably high affinity towards MWCNT. A sample of pure bovine serum albumin was also eluted immediately from the column, while lysozyme did not elute as a peak with PBS, but eluted with 0.6 M NaCl. Fundamentally, carbon nanotubes are devoid of any electrical charge. Therefore, other forces including the hydrogen bonds, hydrophilic interactions, and van der Waals forces were most probably responsible for the differential elution behaviors. In conclusion, this chromatographic method provided a simple and direct analysis of the interactions between carbon nanotubes and the various proteins.

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“…PZP is known to be expressed by a number of different tissue types in humans including the liver, uterus and brain, and is present in cerebral spinal fluid (CSF), synovial fluid and blood plasma (Tayade et al, 2005). The majority of plasma PZP is believed to be synthesised by the liver (Tayade et al, 2005) with plasma levels in both men and women normally < 0.03 mg/ml, but these levels can reportedly increase to ~ 3 mg/mL in some women during pregnancy (Ekelund and Laurell, 1994).…”

Section: B Expression Activation and Turnovermentioning

confidence: 99%

“…The majority of plasma PZP is believed to be synthesised by the liver (Tayade et al, 2005) with plasma levels in both men and women normally < 0.03 mg/ml, but these levels can reportedly increase to ~ 3 mg/mL in some women during pregnancy (Ekelund and Laurell, 1994). At these levels PZP is one of the most abundant proteins in blood plasma.…”

Section: B Expression Activation and Turnovermentioning

confidence: 99%

PZP and PAI-2: Structurally-diverse, functionally similar pregnancy proteins?

Wyatt

Cater

Ranson

2016

The International Journal of Biochemistry & Cell Biology

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Pregnancy zone protein (PZP) and plasminogen activator inhibitor type 2 (PAI-2) are two multifunctional proteins that are elevated in normal pregnancy and numerous other inflammatory states. Both proteins were originally identified as protease inhibitors, but current evidence supports the notion that they may also function as modulators of T-helper cells and/or extracellular chaperones. Exacerbated inflammation, fibrinolytic disturbances and misfolded proteins are all implicated in the pathology of preeclampsia, a leading cause of maternal and foetal mortality and morbidity. Notably, reduced levels of PZP or PAI-2 are associated with preeclampsia and clarification of their diverse functions in normal pregnancy could provide much needed insight regarding the pathogenesis of this disorder. Given that inflammation and protein misfolding underlie the pathology of a very large number of disorders, the contributions of PZP and PAI-2 to extracellular proteostasis and immunoregulation could be broad-reaching. Given that inflammation and protein misfolding underlie the pathology of a very large number of disorders, the contributions of PZP and PAI-2 to extracellular proteostasis and immunoregulation could be broad-reaching.

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Functions of alpha 2 macroglobulins in pregnancy

Cited by 59 publications

References 46 publications

The secretome of MUSE cells contains factors that may play a role in regulation of stemness, apoptosis and immunomodulation

The secretome of MUSE cells contains factors that may play a role in regulation of stemness, apoptosis and immunomodulation

Chromatography of carbon nanotubes separated albumin from other serum proteins: a method for direct analysis of their interactions

PZP and PAI-2: Structurally-diverse, functionally similar pregnancy proteins?

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