Functionally distinct high and low molecular weight species of channel catfish and mouse IL-1

Bacterial lipopolysaccharide (LPS) and interleukin 1 (IL-1) exert multiple physiological effects in the tilapia Oreochromis mossambicus (Teleostei)

“…The comparison between the two experiments shows that the interrenal activa tion was not a requisite for the integumental effect observed, and also identifies the interrenal axis as a tar get of the bacterial product in tilapia. The fact that IL-1 treatment did not result in a similar activation might indicate the different mechanisms of action for in tegumental and endocrine effects of the cytokine prep aration, which likely differs from the tilapia homologue (Ellsaesser and Clem 1994).…”

Cytokines in fish: an update

“…Whilst extreme caution should be used in interpreting results with the former, where non-cytokine factors in supernatants may influence the outcome, the latter approach does seem to have some merit. Some of the first work was carried out with IL-1 in channel catfish (Clem et al, 1991;Ellsaesser & Clem, 1994), and more recently in carp (Verburg van Kemenade et al, 1995). Interestingly, catfish leucocytes were unable to respond to recombinant IL-1 (rIL-1) in a mitogen-induced proliferation assay but could respond to a higher molecular weight form of native IL-1, thought to be an aggregate or polymer (Ellsaesser & Clem, 1994).…”

“…Some of the first work was carried out with IL-1 in channel catfish (Clem et al, 1991;Ellsaesser & Clem, 1994), and more recently in carp (Verburg van Kemenade et al, 1995). Interestingly, catfish leucocytes were unable to respond to recombinant IL-1 (rIL-1) in a mitogen-induced proliferation assay but could respond to a higher molecular weight form of native IL-1, thought to be an aggregate or polymer (Ellsaesser & Clem, 1994). Responsiveness of fish leucocytes to rTNF has also been demonstrated (Hardie et al, 1994a;Jang et al, 1995b), although the e#ect generally required Table 2.…”

“…) (3) Antigenic cross-reactivity (a) to cytokines (e.g. Ahne, 1993Ahne, , 1994aEllsaesser & Clem, 1994;Robertsen et al, 1994;Jang et al, 1995a.) (b) to cytokine receptors (e.g.…”

“…) (2) Biological cross-reactivity (a) fish leucocyte-derived supernatants on mammalian cell assays (e.g. Ellsaesser & Clem, 1994;Verburg van Kemenade et al, 1995.) (b) mammalian cytokines on fish cell assays (e.g.…”