Functionalized silk fibroin dressing with topical bioactive insulin release for accelerated chronic wound healing

Li, Xiufang; Yan, Lei; Zhang, Jian; You, Renchuan; Qu, Jing; Li, Mingzhong

doi:10.1016/j.msec.2016.11.085

Cited by 80 publications

(57 citation statements)

References 36 publications

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“…procedures were consistent with our previous methods (X. Li et al, 2017;Yan et al, 2013). The process of the surgical implantation of scaffolds into full-thickness wounds of SD rats is shown in Figure S1.…”

Section: Cam Assaysupporting

confidence: 86%

Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

Wang

Jiang

et al. 2019

J Tissue Eng Regen Med

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Vascularization remains a critical challenge in dermal tissue regeneration. In this study, a vascular endothelial growth factor (VEGF165) and angiopoietin‐1 (Ang‐1) dual gene coexpression vector that encoded green fluorescent protein (GFP) was constructed from an arginine–glycine–aspartic acid‐modified adenovirus. Silk fibroin (SF) scaffolds loaded with adenovirus vectors were fabricated by freeze‐drying method. In vitro, the human endothelial‐derived cell line EA.hy926 was infected with adenovirus vectors and then expressed GFP, secreted VEGF165 and Ang‐1, and promoted cell proliferation effectively. The VEGF165 and Ang‐1 genes loaded in the SF scaffolds significantly promoted the formation of abundant microvascular networks in the chick embryo chorioallantoic membrane. In vivo, angiogenic genes loaded in the scaffolds promoted vascularization and collagen deposition in scaffolds, thus effectively accelerating dermal tissue regeneration in a dorsal full‐thickness skin defect wound model in Sprague–Dawley rats. In conclusion, SF scaffolds loaded with arginine–glycine–aspartic acid‐modified adenovirus vectors encoding VEGF165 and Ang‐1 could stimulate the formation of vascular networks through the effective expression of target genes in vascular endothelial cells, thereby accelerating the regeneration of dermal tissue.

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Section: Cam Assaysupporting

confidence: 86%

Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

Wang

Jiang

et al. 2019

J Tissue Eng Regen Med

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“…In addition to SF electrospun mats with rather simple structure, multicomponent SF-based composite systems have been widely researched. Insulin-loaded SF microspheres encapsulated within SF sponges have been reported for the treatment of chronic cutaneous wounds [148]. Owing to the stimulatory effect of insulin, keratinocytes and endothelial cells could exhibit increased proliferation and differentiation.…”

Section: Biomedical Applications Of Sf-based Materialsmentioning

confidence: 99%

Silk Fibroin-Based Biomaterials for Biomedical Applications: A Review

et al. 2019

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Since it was first discovered, thousands of years ago, silkworm silk has been known to be an abundant biopolymer with a vast range of attractive properties. The utilization of silk fibroin (SF), the main protein of silkworm silk, has not been limited to the textile industry but has been further extended to various high-tech application areas, including biomaterials for drug delivery systems and tissue engineering. The outstanding mechanical properties of SF, including its facile processability, superior biocompatibility, controllable biodegradation, and versatile functionalization have allowed its use for innovative applications. In this review, we describe the structure, composition, general properties, and structure-properties relationship of SF. In addition, the methods used for the fabrication and modification of various materials are briefly addressed. Lastly, recent applications of SF-based materials for small molecule drug delivery, biological drug delivery, gene therapy, wound healing, and bone regeneration are reviewed and our perspectives on future development of these favorable materials are also shared.

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“…Despite the aforementioned properties of GL, ganoderma lucidum spores polysaccharide (GLSP) have been identified for antidiabetic and tissue repair benefits, especially for the treatment of chronic cutaneous wounds caused by diabetes mellitus and vascular indications (e.g. such as diabetic foot ulcers), which carry the risk of limb amputation [16,17]. In-vivo experiments of GLSP present no toxicity, implying good biocompatibility, while in practice, β-D-glucan (one form of polysaccharide isolated from GLSP) is highly sensitive to oxidative degradation, including the reactions of active oxygen substances, such as hydroxyl radicals [18].…”

Section: Introductionmentioning

confidence: 99%

Designer fibers from 2D to 3D – Simultaneous and controlled engineering of morphology, shape and size

Yao

Zhang

Ahmad

et al. 2018

Chemical Engineering Journal

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Topography and surface morphology of micrometer and nanometer scaled fibrous biomaterials are crucial for bioactive component encapsulation, release, promoting cell proliferation and interaction within biological environment. Specifically, for drug delivery and tissue repair applications, surface engineering provides control on both aspects in tandem. In this study, the bioactive component (ganoderma lucidum spore polysaccharide (GLSP)) was loaded into zein prolamine (ZP) fiber matrices via coaxial electrospinning (CES) technique. During the CES process, various outer layer enveloping fluids were used to modulate fiber topography in-situ (from 2D to 3D). SEM and water contact angle tests indicate enveloping media impact electrospun fiber diameter (ranging from 400 nm to 3.0 μm) and morphologies (from flat ribbon-like to solid cylindrical structures), with the latter impacting GLSP release profile. Furthermore, CCK-8 assay assessment indicates fibroblast cell proliferation (L929 cell line), while cell extension was also observed for modified ZP fibers. The results demonstrate potential applications of modified fiber morphologies, which are tailored in-situ without impacting chemical stability and encapsulation.

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Functionalized silk fibroin dressing with topical bioactive insulin release for accelerated chronic wound healing

Cited by 80 publications

References 36 publications

Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

Silk Fibroin-Based Biomaterials for Biomedical Applications: A Review

Designer fibers from 2D to 3D – Simultaneous and controlled engineering of morphology, shape and size

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