Abstract:We present a biomimetic and reusable microsensor with sub-nanomolar sensitivity by elaboratly functionalizing graphene for monitoring NO release in real-time.
“…These results reveal that FGPC/AN has a satisfactory selectivity toward NO. The good selectivity might be attributed to the highly inherent specificity of the ferriporphyrin to electrocatalytic oxidation of NO, which is in good agreement with the previous report 40 . Figure 3 ( a ) Amperometric response of the FGPC/AN to interference substances in deaerated PBS solutions, in which all species were at the concentration of 1 mM.…”
Section: Resultssupporting
confidence: 92%
“…For example, Liu et al . 40 has constructed a microelectrode array sensor based on metalloporphyrin functionalized graphene, and realized ultra sensitive real-time monitoring of NO release from living cells. Jiang et al .…”
Section: Introductionmentioning
confidence: 99%
“…Therefore, the catalytic capability of porphyrin or hemin functionalized with nanomaterials can greatly enhance the detection sensitivity to NO. Although NO electrochemical sensors have been reported from various living cells 27 , 40 , none of them has been utilized for real-time monitoring of NO in local acupoints. In our previous work 41 , 42 , we have successfully fabricated nanomaterials modified acupuncture needle by electrochemical deposition of graphene or CNT on the surface of acupuncture needle, and explored electrochemical measurements for sensitive detection of dopamine and serotonin, respectively.…”
This study reports an acupuncture needle modified with an iron-porphyrin functionalized graphene composite (FGPC) for real-time monitoring of nitric oxide (NO) release in acupoints of rats. A gold film was first deposited to the needle surface to enhance the conductivity. The FGPC was prepared via hydrothermal synthesis, and subsequently applied to the tip surface of acupuncture needle by electrochemical deposition method. The functionalized needle enabled a specific and sensitive detection of NO based on the favorably catalytic properties of iron-porphyrin and the excellent conductivity of graphene. Amperometric data showed that the needle achieved not only a low detection limit down to 3.2ânM in PBS solution, but also a satisfactory selectivity. Interestingly, the functionalized needle could be inserted into the acupoints of rats for real-time monitoring of NO in vivo. It was found that a remarkable response to NO was respectively obtained in different acupoints when stimulated by L-arginine (L-Arg), revealing that the release of NO was detectable in acupoints. We expect this work would showcase the applications of acupuncture needle in detecting some important signaling molecules in vivo, and exploring the mechanism of acupuncture treatment.
“…These results reveal that FGPC/AN has a satisfactory selectivity toward NO. The good selectivity might be attributed to the highly inherent specificity of the ferriporphyrin to electrocatalytic oxidation of NO, which is in good agreement with the previous report 40 . Figure 3 ( a ) Amperometric response of the FGPC/AN to interference substances in deaerated PBS solutions, in which all species were at the concentration of 1 mM.…”
Section: Resultssupporting
confidence: 92%
“…For example, Liu et al . 40 has constructed a microelectrode array sensor based on metalloporphyrin functionalized graphene, and realized ultra sensitive real-time monitoring of NO release from living cells. Jiang et al .…”
Section: Introductionmentioning
confidence: 99%
“…Therefore, the catalytic capability of porphyrin or hemin functionalized with nanomaterials can greatly enhance the detection sensitivity to NO. Although NO electrochemical sensors have been reported from various living cells 27 , 40 , none of them has been utilized for real-time monitoring of NO in local acupoints. In our previous work 41 , 42 , we have successfully fabricated nanomaterials modified acupuncture needle by electrochemical deposition of graphene or CNT on the surface of acupuncture needle, and explored electrochemical measurements for sensitive detection of dopamine and serotonin, respectively.…”
This study reports an acupuncture needle modified with an iron-porphyrin functionalized graphene composite (FGPC) for real-time monitoring of nitric oxide (NO) release in acupoints of rats. A gold film was first deposited to the needle surface to enhance the conductivity. The FGPC was prepared via hydrothermal synthesis, and subsequently applied to the tip surface of acupuncture needle by electrochemical deposition method. The functionalized needle enabled a specific and sensitive detection of NO based on the favorably catalytic properties of iron-porphyrin and the excellent conductivity of graphene. Amperometric data showed that the needle achieved not only a low detection limit down to 3.2ânM in PBS solution, but also a satisfactory selectivity. Interestingly, the functionalized needle could be inserted into the acupoints of rats for real-time monitoring of NO in vivo. It was found that a remarkable response to NO was respectively obtained in different acupoints when stimulated by L-arginine (L-Arg), revealing that the release of NO was detectable in acupoints. We expect this work would showcase the applications of acupuncture needle in detecting some important signaling molecules in vivo, and exploring the mechanism of acupuncture treatment.
“…Graphene and its derivatives represent an increasingly attractive delivery system and the strategy of covalent and non-covalent functionalization seems to increase the stability, the solubility, the biocompatibility and biological applications [ 60 ]. On the one hand, graphene decoration influences the cell growth, the ROS release or the secretion of nitric oxide (NO) in order to differentiate cancer cells and normal cells [ 33 , 61 , 62 , 63 , 64 , 65 , 66 ]. Graphene functionalization can promote drug delivery and increase the tissue specificity of the delivery systems in order to enhance the drug bioavailability and overcome multidrug resistance in conventional cancer therapy [ 67 , 68 , 69 ].…”
Section: Intracellular Molecular Pathways Activated By Functional mentioning
The paper reviews the network of cellular signaling pathways activated by Functional Graphene Nanomaterials (FGN) designed as a platform for multi-targeted therapy or scaffold in tissue engineering. Cells communicate with each other through a molecular device called signalosome. It is a transient co-cluster of signal transducers and transmembrane receptors activated following the binding of transmembrane receptors to extracellular signals. Signalosomes are thus efficient and sensitive signal-responding devices that amplify incoming signals and convert them into robust responses that can be relayed from the plasma membrane to the nucleus or other target sites within the cell. The review describes the state-of-the-art biomedical applications of FGN focusing the attention on the cell/FGN interactions and signalosome activation.
“…Graphene oxide, as a graphene-based inorganic carbon material, possesses many exceptional properties including nanoscale controllability and biocompatibility which merit attention for many potential applications. As a robust yet flexible material it provides many possibilities for facile modification and fabrication to produce other desired graphene based materials [1][2][3][4][5][6][7]. Recently, graphene has advanced its applications in biomedical and energy engineering [8][9][10].…”
Fluorescent materials are important for low-cost opto-electronic and biomedical sensor devices. In this study we present the synthesis and characterization of graphene modified with bis-thiosemicarbazone (BTS). This new material was characterized using Fourier transform infrared spectroscopy (FT-IR), Ultraviolet-visible (UV-Vis) and Raman spectroscopy techniques. Further evaluation by X-ray diffraction (XRD), thermo-gravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and atomic-force microscopy (AFM) allowed us to fully characterize the morphology of the fabricated material. The average height of the BTSGO sheet is around 10nm. Optical properties of BTSGO evaluated by photoluminescence (PL) spectroscopy showed red shift at different excitation wavelength compared to graphene oxide or bisthiosemicarbazide alone. These results strongly suggest that BTSGO material could find potential applications in graphene based optoelectronic devices.
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