Functionalized Electrospun Double-Layer Nanofibrous Scaffold for Wound Healing and Scar Inhibition

Abstract

Considerable advances have been made in developing materials that promote wound healing and inhibit scar formation in clinical settings. However, some challenges, such as cumbersome treatment processes and determination of optimal treatment time, remain unresolved. Thus, developing a multifunctional wound dressing with both wound healing and scar inhibition properties is crucial. Here, we present an integrated electrospun fibrous composite membrane (MPC12) for wound healing and scar inhibition, consisting of a quaternized chitosan-loaded inner membrane (PCQC5) and quaternized silicone-loaded outer membrane (MQP12). The inner membrane effectively coagulates blood and promotes wound healing, and the outer membrane moisturizes, resists bacteria, and inhibits scar formation. In vivo evaluation in a rabbit ear model revealed that MPC12 treatment results in faster wound healing and better alleviation of scar hypertrophy than treatment with commercial products (KELO-COTE and MSSG). Our strategy offers an excellent solution for the potential integration of wound healing and scar inhibition.

Chitosan-Based Smart Biomaterials for Biomedical Applications: Progress and Perspectives

“…141 Therefore, Su et al fabricated an electrospun double-layer nanofibrous scaffold (MPC 12) for wound healing and scar inhibition as shown in Figure 15. 142 The fibrous scaffold consisted of Quaternized silicone (QP12) as the outer layer to prevent harmful bacteria, debris, and water penetrating the wound and PVC/collagen/quaternized chitosan membrane (PCQ5) as the inner layer to promoting cell adhesion, proliferation, as well as wound repair. The in vivo experiment shows that 96.20% wound closure was achieved within 14 days and the skin was completely healed without a scar within 50 days.…”

“…Wound dressing that could prevent scars formation is highly demanded since tissue scarring, connective tissue response to surgery, trauma, inflammation, or burn injuries are persistent challenges for dermatologists and plastic surgeons . Therefore, Su et al fabricated an electrospun double-layer nanofibrous scaffold (MPC 12) for wound healing and scar inhibition as shown in Figure . The fibrous scaffold consisted of Quaternized silicone (QP12) as the outer layer to prevent harmful bacteria, debris, and water penetrating the wound and PVC/collagen/quaternized chitosan membrane (PCQ5) as the inner layer to promoting cell adhesion, proliferation, as well as wound repair.…”

“…Schematic illustration of MPC12 nanofiber composite membrane design for the integration of wound healing and scar inhibition in a rabbit ear wound model. Reproduced with permission from ref . Copyright 2022 American Chemical Society.…”

Animal tissue-derived biomaterials for promoting wound healing

“…Although these biomaterials all showed effective results in promoting wound healing, scar-free recovery is still quite difficult. [84][85][86] To further achieve scar-free therapy, Yan et al proposed a novel scaffold by simulating the biochemical constituents and structures of ECM with hyaluronic acid (HA) and silk fibroin nanofibers (SNFs). HUVECs were then cultivated in this scaffold (Fig.…”

An Injectable Integration of Autologous Bioactive Concentrated Growth Factor and Gelatin Methacrylate Hydrogel with Efficient Growth Factor Release and 3D Spatial Structure for Accelerated Wound Healing

“…Delayed wound repair following trauma, surgery, burns, scalds, and chronic infections in diabetes affects the quality of life of individuals, contributing to psycho‐social burden worldwide. [ 1,2 ] Poor homeostasis, wound infection, and scar‐free wound repair remain persistent challenges. Wound healing encompasses the following temporally overlapping phases: a) hemostasis, b) local inflammatory response, c) cell proliferation and differentiation and granulation formation, and d) tissue remodeling.…”