Silver nanoparticles (NPs) have been synthesized by aqueous Beetroot extract. Effective parameters on the synthesis of silver NPs like pH, extract volume, silver nitrate concentration and time of reaction have been investigated at room temperature. The synthesized silver NPs have been studied with different analysis like UV-Vis, FT-IR, FE-SEM, TEM, and XRD. Our analysis indicates that the level of Surface Plasmon is 439 nm. The shape of NPs is spherical and the size is 20-30 nm using FE-SEM and TEM technique. Four bacteria (Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Salmonella typhymurium) were used as references bacteria to analyze the antibacterial effect. Antibacterial results show that gram negative bacteria are so sensitive to synthesize silver NP. The recommended method was completely eco-friendly.