Functionalization of graphene family nanomaterials for application in cancer therapy

Melo‐Diogo, Duarte de; Lima‐Sousa, Rita; Alves, Cátia G.; Costa, Elisabete C.; Louro, Ricardo O.; Correia, Ilídio J.

doi:10.1016/j.colsurfb.2018.07.030

Cited by 69 publications

(54 citation statements)

References 121 publications

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“…2,4 Additionally, GO can be functionalized with different molecules such as dispersants, imaging agents, targeting and therapeutic molecules, making this material a perfect platform for theragnostic and multimodal applications. 2,4 However, several issues still need to be addressed. Currently, application of GO in the biomedical field is ruled by the dichotomy between efficiency of the treatment (in vivo or in vitro) and the GO toxicity.…”

Section: Introductionmentioning

confidence: 99%

“Ultramixing”: A Simple and Effective Method To Obtain Controlled and Stable Dispersions of Graphene Oxide in Cell Culture Media

Reina

Ruiz

Murera

et al. 2019

ACS Appl. Mater. Interfaces

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The last decade has seen an increase in the application of graphene oxide (GO) in the biomedical field. GO has been successfully exploited for its ability to deliver many kinds of drugs into target cells. However, GO toxicity assessment is still controversial. Several studies demonstrated that GO protein coating is crucial to alleviate the material's toxicity. Besides, coronation leads to the formation of big agglomerates, reducing the cellular uptake of the material and thus its therapeutic efficiency. In this work, we propose a simple and efficient method based on rapid (Ultra-Turrax, UT) mixing to control protein corona formation. Using UT protocol, we were able to reduce GO agglomeration in the presence of proteins and to obtain stable GO dispersions in cell culture media. By labelling GO with luminescent nanoparticles (QDs), we studied the GO internalization kinetic and efficiency. Comparing the "classic" and the UT protocols, we found that the latter allows a faster and more efficient internalization both in macrophages and HeLa cells without affecting cell viability. We believe that the use of UT protocol will be interesting and suitable for the preparation of next generation GO-based drug delivery platforms. Recherche (ANR) through the LabEx project Chemistry of Complex Systems (ANR-10-LABX-0026_CSC).

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Section: Introductionmentioning

confidence: 99%

“Ultramixing”: A Simple and Effective Method To Obtain Controlled and Stable Dispersions of Graphene Oxide in Cell Culture Media

Reina

Ruiz

Murera

et al. 2019

ACS Appl. Mater. Interfaces

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“…19 Besides, the addition of rGO in the mixing solution usually causes a poor water solubility and undesired aggregation which can adversely affect cellular behaviour and fate. 20 To address these problems, the aim of the present study is twofold. Firstly, it attempts to improve the fabrication method of the 3D graphene-alginate composite scaffolds that uses a simple, scalable and environmentally sustainable fabrication technique involving solution mixing, freeze-drying, crosslinking, and bio-reduction.…”

Section: Introductionmentioning

confidence: 99%

Advancing fabrication and properties of three-dimensional graphene–alginate scaffolds for application in neural tissue engineering

et al. 2019

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“…The acid‐labile D‐DOX was designed as drug–drug conjugate for tumor intracellular pH‐triggered release, by conjugating two doxorubicin (DOX) molecules with one ADH molecule through acid‐labile hydrazone linkage between the carbonyl group in DOX and the hydrazide groups in ADH (Scheme ). To endow the blood stability, improve the pharmacokinetic and pharmacodynamic outcomes of therapeutics, and tailor the particles size of the proposed DSDS prodrug nanoparticles by micellization, the D‐DOX dimer was mono‐PEGylated or bi‐PEGylated with methoxy‐poly(ethylene glycol)‐carboxylic acid (mPEG‐COOH) as prodrug surfactant, via the amidation on one or two amino groups in the D‐DOX dimer. The success synthesis of the D‐DOX dimer and the PEGylated D‐DOX prodrug surfactants were revealed by the 1 H NMR analysis ( Figures and ).…”

Section: Resultsmentioning

confidence: 99%

Self‐Assembly of Drug–Drug Conjugates as Drug Self‐Delivery System for Tumor‐Specific pH‐Triggered Release

Liu

2019

Part & Part Syst Charact

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An acid‐labile doxorubicin dimer (D‐DOX) is designed as drug–drug conjugate for tumor intracellular pH‐triggered release, by conjugating doxorubicin (DOX) with adipic acid dihydrazide (ADH). The dimer‐based surfactants modified with polyethylene glycol (PEG), DOX‐ADH‐DOX‐PEG or are synthesized by mono‐PEGylation and bi‐PEGylation, respectively. Then the prodrug nanoparticles are fabricated with different drug contents via dialyzing the mixture solution of D‐DOX and the PEGylated surfactants in dimethyl sulfoxide (DMSO) with different mass ratios against water. It is found that the smaller prodrug nanoparticles (142–163 nm) could be obtained with the mono‐PEGylated surfactant, than those of 157–225 nm with the bi‐PEGylated surfactant. Furthermore, the mono‐PEGylated surfactant results in a higher drug content of 51% due to their lower PEG contents. All prodrug nanoparticles could release DOX completely within 36 h at pH 5.0, with the premature drug leakage of less than 10% at pH 7.4. The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assays demonstrate the proposed drug self‐delivery system possessed an enhanced anticancer efficacy against HepG2 cells than the free DOX.

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Functionalization of graphene family nanomaterials for application in cancer therapy

Cited by 69 publications

References 121 publications

“Ultramixing”: A Simple and Effective Method To Obtain Controlled and Stable Dispersions of Graphene Oxide in Cell Culture Media

“Ultramixing”: A Simple and Effective Method To Obtain Controlled and Stable Dispersions of Graphene Oxide in Cell Culture Media

Advancing fabrication and properties of three-dimensional graphene–alginate scaffolds for application in neural tissue engineering

Self‐Assembly of Drug–Drug Conjugates as Drug Self‐Delivery System for Tumor‐Specific pH‐Triggered Release

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