Functionalization of Gold and Glass Surfaces with Magnetic Nanoparticles Using Biomolecular Interactions

Abstract

Advances in nanotechnology have enabled the production and characterization of magnetic particles with nanometer-sized features that can be functionalized with biological recognition elements for numerous applications in biotechnology. In the present study, the synthesis of and interactions between self-assembled monolayers (SAMs) on gold and glass surfaces and functionalized magnetic nanoparticles have been characterized. Immobilization of 10-15 nm streptavidin-functionalized nanoparticles to biotinylated gold and glass surfaces was achieved by the strong interactions between biotin and streptavidin. Fluorescent streptavidin-functionalized nanoparticles, biotinylated surfaces, and combinations of the two were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and electron and fluorescent microscopy to confirm that little or no functionalization occurred in nonbiotinylated regions of the gold and glass surfaces compared to the biotinylated sites. Together these techniques have potential use in studying the modification and behavior of functionalized nanoparticles on surfaces in biosensing and other applications.

Application of Iron Magnetic Nanoparticles in Protein Immobilization

“…The proteins functionalized with (strept)avidin are unusually stable to extremes such as heat, denaturants, pH, and proteolysis, indicating the binding is essentially irreversible. For instance, Nidumolu’s group reported the synthesis of streptavidin-functionalized magnetic nanoparticles and investigation of the binding to biotinylated SAMs on gold and glass for biological recognition applications [148]. The whole process includes three steps.…”

Bio and Nanomaterials Based on Fe3O4

“…Fe 3 O 4 nanoparticles have been intensively utilized to realize this objective due to its unique magnetic performance, and various practical and economical biocatalysts with improved stability and reusability have been fabricated based on Fe 3 O 4 nanoparticles, which could be easily separated from the reaction medium in the presence of external magnetic field [1,2,3,4,5,6,7,8,9,10,95,96,97,98,99,100,101,102,103,104,105,106,107,108,109,110,111,112,113,114]. Proteins could be immobilized onto Fe 3 O 4 nanoparticles in the manner of physical absorption [95,96,97], covalent bonding [98,99,100,101,102,103,104], and bioconjugation [105,106,107]. Coupling reagents, such as glutaraldehyde [99,100,101,102,108,109,110], 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) [103,104,111,112,113] and sodium tripolyphosphate (TPP) [114], are often utilized to achieve much more stable immobilization via covalent bon...…”

Theory and Applications of Surface Plasmon Resonance, Resonant Mirror, Resonant Waveguide Grating, and Dual Polarization Interferometry Biosensors

“…Protein-carbohydrate interactions can also be monitored by glycan-modified surfaces [35,36]. By taking advantage of the extremely strong affinity of biotin for avidin or streptavidin, biotinylated surfaces [37] can be particularly useful for capturing labeled proteins, as we have recently demonstrated [38]. Nickel nitrilotriacetic acid (Ni-NTA)-derivatized surfaces [39,40] are also convenient for specific capturing of proteins that have been genetically engineered with an N- or C-terminal polyhistidine tag, a common affinity moiety used during protein expression and purification processes.…”