Functionality of decellularized matrix in cartilage regeneration: A comparison of tissue versus cell sources

Sun, Yu; Yan, Lianqi; Chen, Song; Pei, Ming

doi:10.1016/j.actbio.2018.04.048

Cited by 74 publications

(57 citation statements)

References 248 publications

(317 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This method takes advantage of the natural bioactive properties of tissue to produce a suitable scaffold for stem cell culture and differentiation. The methods used to process the tissue may vary and can result in different properties for the scaffold material obtained [193] and they vary based on the tissue targeted [194]. Such dECM scaffolds have been extensively characterized for tissue engineering applications involving MSCs [195,196].…”

Section: Decellularized Extracellular Matrixmentioning

confidence: 99%

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Willerth

Sakiyama‐Elbert

2019

STJ

111

113

View full text Add to dashboard Cite

Combining stem cells with biomaterial scaffolds serves as a promising strategy for engineering tissues for both in vitro and in vivo applications. This updated review details commonly used biomaterial scaffolds for engineering tissues from stem cells. We first define the different types of stem cells and their relevant properties and commonly used scaffold formulations. Next, we discuss natural and synthetic scaffold materials typically used when engineering tissues, along with their associated advantages and drawbacks and gives examples of target applications. New approaches to engineering tissues, such as 3D bioprinting, are described as they provide exciting opportunities for future work along with current challenges that must be addressed. Thus, this review provides an overview of the available biomaterials for directing stem cell differentiation as a means of producing replacements for diseased or damaged tissues.

show abstract

Section: Decellularized Extracellular Matrixmentioning

confidence: 99%

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Willerth

Sakiyama‐Elbert

2019

STJ

111

113

View full text Add to dashboard Cite

show abstract

“…4 and Table 8. (11) . They are highly differentiated cells whose main secretions are collagen (mainly type II collagen) and proteoglycans.…”

Section: Resultsmentioning

confidence: 99%

Guyanxiao formula antagonizes LPS-induced KOA chondrocyte injury by regulating the SDF-1 / CXCR4 signaling pathway1

Zhang

Shu-guang

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background To determine whether Guyanxiao formula protects chondrocytes in a model of knee arthritis induced by lipopolysaccharide, and whether it can repair chondrocyte damage and suppress osteoarthritis cartilage degeneration by regulating SDF-1 / CXCR4 signaling pathway.Methods Lipopolysaccharide(LPS) induces chondrocytes in vitro to prepare knee osteoarthritis model. Toluidine blue (TBS) staining was used to observe the changes of proteoglycan content of rabbit chondrocytes in order to identify the source of cells. The biochemical detection method was used to determine the content of inflammatory factor nitric oxide (NO) in chondrocytes to identify whether the osteoarthritis chondrocytes were successfully modeled in vitro.The cell proliferation rate was measured by the cell viability test (CCK-8), the concentration with no obvious cytotoxicity was screened, and the low, medium and high dose groups of Guyanxiao formula were established.Immunofluorescence(IF) staining was used to observe the effect of Guyanxiao formula on the content of type Ⅱ collagen in chondrocytes of knee osteoarthritis.Enzyme-linked immunosorbent assay was carried out to determine the expression of inflammatory factors MMP-3, MMP-9 and MMP-13. The mRNA and protein expressions of SDF-1, CXCR4, Vascular endothelial growth factor(VEGF) were analyzed by reverse transcription quantitative polymerase chain reaction and Western blot analysis.Results The identify of chondrocytes was confirmed with toluidine blue staining. LPS treatment remarkably increased the NO content, indicating successful noodling of the KOA chondrocyte model. According to the CCK-8 experiment results, 0.36, 3.6, and 36 µg / mL were set as the low, medium, and high dose administration concentrations of ostitis.Immunofluorescence(IF) staining showed that the degree of type Ⅱ collagen damage in each treatment group was improved compared with the model group, and the high concentration group was the most obvious improvement in the Guyanxiao formula treatment group.The levels of MMP-3,MMP-9, MMP-13, and IL-1b were much lower in the Cell supernatant of the each treatment group than in that of model group.The levels of SDF-1, CXCR4, VEGF mRNA and protein were much lower in the Chondrocytes of the each treatment group than in that of model group. In addition, the therapeutic effect of Guyanxiao formula treatment group decreased in a concentration-dependent manner.Conclusion Guyanxiao formula antagonizes LPS-induced KOA chondrocyte injury by regulating the SDF-1 / CXCR4 signaling pathway.

show abstract

“…Cartilage tissue formation was faster in decellularized CDM membranes with an increased hyaline-like characteristic. [180] In summary, CDMs have been demonstrated to be more effective in cartilage regeneration than tissue dECMs and synthetic scaffolds coated with ECM (reviewed by Sun et al [181] ).…”

Section: Skeletal Tissuementioning

confidence: 99%

Engineering Cell‐Derived Matrices: From 3D Models to Advanced Personalized Therapies

Rubí-Sans

Castaño

Cano

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

Regenerative medicine and disease models have evolved in recent years from two to three dimensions, providing in vitro constructs that are more similar to in vivo tissues. By mimicking native tissues, cell-derived matrices (CDMs) have emerged as new modifiable extracellular matrices for a variety of tissues, allowing researchers to study basic cellular processes in tissue-like structures, test tissue regeneration approaches, and model disease development. In this review, different fabrication techniques and characterization methods of CDMs are presented and examples of their application in cell behavior studies, tissue regeneration, and disease models are provided. In addition, future guidelines and perspectives in the field of CDMs are discussed.

show abstract

Functionality of decellularized matrix in cartilage regeneration: A comparison of tissue versus cell sources

Cited by 74 publications

References 248 publications

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Guyanxiao formula antagonizes LPS-induced KOA chondrocyte injury by regulating the SDF-1 / CXCR4 signaling pathway1

Engineering Cell‐Derived Matrices: From 3D Models to Advanced Personalized Therapies

Contact Info

Product

Resources

About