Engineering Cell‐Derived Matrices: From 3D Models to Advanced Personalized Therapies

Rubí-Sans, Gerard; Castaño, Óscar; Cano, Irene; Mateos‐Timoneda, Miguel A.; Pérez‐Amodio, Soledad; Engel, Elisabeth

doi:10.1002/adfm.202000496

Cited by 16 publications

(10 citation statements)

References 200 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 65,66 ] In top‐down approaches, cells are seeded on top of a preformed scaffold. [ 63 ] Among them, fibers, [ 67,68 ] foams, [ 69 ] porous/solid microparticles [ 70–72 ] and decellularized cell‐derived and tissues/organs‐derived matrix scaffolds [ 73,74 ] have been widely used. The main limitation of these scaffolds is related to the necessity of cells infiltration into the structure, an important technical aspect, that generally leads to difficulties in guaranteeing a homogeneous cell distribution and infiltration throughout the whole structure.…”

Section: In Vitro Models To Recapitulate the Tmementioning

confidence: 99%

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

et al. 2021

Self Cite

View full text Add to dashboard Cite

The establishment of tumor microenvironment using biomimetic in vitro models that recapitulate key tumor hallmarks including the tumor supporting extracellular matrix (ECM) is in high demand for accelerating the discovery and preclinical validation of more effective anticancer therapeutics. To date, ECM‐mimetic hydrogels have been widely explored for 3D in vitro disease modeling owing to their bioactive properties that can be further adapted to the biochemical and biophysical properties of native tumors. Gathering on this momentum, herein the current landscape of intrinsically bioactive protein and peptide hydrogels that have been employed for 3D tumor modeling are discussed. Initially, the importance of recreating such microenvironment and the main considerations for generating ECM‐mimetic 3D hydrogel in vitro tumor models are showcased. A comprehensive discussion focusing protein, peptide, or hybrid ECM‐mimetic platforms employed for modeling cancer cells/stroma cross‐talk and for the preclinical evaluation of candidate anticancer therapies is also provided. Further development of tumor‐tunable, proteinaceous or peptide 3D microtesting platforms with microenvironment‐specific biophysical and biomolecular cues will contribute to better mimic the in vivo scenario, and improve the predictability of preclinical screening of generalized or personalized therapeutics.

show abstract

Section: In Vitro Models To Recapitulate the Tmementioning

confidence: 99%

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cell 3D bio-printing allows mimicking native tissues. Cell-derived matrices (CDMs) are a new type of modifiable extracellular matrices for a variety of tissues [47]. Currently, it is possible to fabricate tissues and organs by depositing live cells with ink droplets, although they may lack some elements.…”

Section: D Bio-printingmentioning

confidence: 99%

Use of FDM Technology in Healthcare Applications: Recent Advances

Buj-Corral

Tejo-Otero

Fenollosa-Artés

2021

Materials Forming, Machining and Tribology

View full text Add to dashboard Cite

Additive Manufacturing (AM) technologies, which were developed around 30 years ago, are still evolving. They are applied in different sectors such as aeronautics, automotive, health, etc. There are different AM technologies: binder jetting, direct energy deposition, material extrusion, material jetting, powder bed fusion, sheet lamination and vat photopolimerisation. Amongst all of them, material extrusion is the most common technique used nowadays, due to several reasons: manufacture of parts is easy and cost-effective, desktop 3D printers are affordable, and they allow the use of many different materials. Within this AM category, two main techniques can be highlighted: FFF (Fused Filament Fabrication), also known as FDM (Fused Deposition Modelling), and DIW (Direct Ink Writing). FFF is the most typical technology which can offer multimaterial 3D printed parts. In addition, it can be mixed with other AM technologies in order to build hybrid 3D printers. Main applications of the FFF technology in the medical sector, which are explained in detail in the present chapter, are the manufacture of training models and surgical planning prototypes, medical devices, surgical guides, bio-active scaffolds, and cell 3D-bioprinting, etc. During the global pandemic of COVID-19, the FDM technology allowed to print different devices such as face masks or artificial breathers, among others.

show abstract

“…CDMs can be produced using different cell sources, and repopulation can be done with cell types of choice, which allows engineering of specific tissue and disease in vitro models . In addition, CDM recellularization with patient’s cells opens a door to develop patient-specific in vitro models as a personalized platform for drug screening and therapeutic target identification. , However, most of the mentioned methods produce CDMs as flat ECM sheets or microfabricated patterns, with cells grown on top as a monolayer, a successful strategy used in skin regeneration or wound healing. , To provide a more complex 3D architecture, Chiang et al used mesenchymal stem cell (MSC) spheroids to generate 3D CDMs, and it showed successful repopulation with endothelial cells around the spherical shape . However, the spherical CDMs reached a maximum size of 200 μm in diameter and required aggregation to form microtissues (∼0.5–1 mm).…”

Section: Introductionmentioning

confidence: 99%

Development of Cell-Derived Matrices for Three-Dimensional In Vitro Cancer Cell Models

Rubí-Sans

Nyga

Rebollo

et al. 2021

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Most morphogenetic and pathological processes are driven by cells responding to the surrounding matrix, such as its composition, architecture, and mechanical properties. Despite increasing evidence for the role of extracellular matrix (ECM) in tissue and disease development, many in vitro substitutes still fail to effectively mimic the native microenvironment. We established a novel method to produce macroscale (>1 cm) mesenchymal cell-derived matrices (CDMs) aimed to mimic the fibrotic tumor microenvironment surrounding epithelial cancer cells. CDMs are produced by human adipose mesenchymal stem cells cultured in sacrificial 3D scaffold templates of fibronectin-coated poly-lactic acid microcarriers (MCs) in the presence of macromolecular crowders. We showed that decellularized CDMs closely mimic the fibrillar protein composition, architecture, and mechanical properties of human fibrotic ECM from cancer masses. CDMs had highly reproducible composition made of collagen types I and III and fibronectin ECM with tunable mechanical properties. Moreover, decellularized and MC-free CDMs were successfully repopulated with cancer cells throughout their 3D structure, and following chemotherapeutic treatment, cancer cells showed greater doxorubicin resistance compared to 3D culture in collagen hydrogels. Collectively, these results support the use of CDMs as a reproducible and tunable tool for developing 3D in vitro cancer models.

show abstract

Engineering Cell‐Derived Matrices: From 3D Models to Advanced Personalized Therapies

Cited by 16 publications

References 200 publications

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

Use of FDM Technology in Healthcare Applications: Recent Advances

Development of Cell-Derived Matrices for Three-Dimensional In Vitro Cancer Cell Models

Contact Info

Product

Resources

About