Functionality of a next generation biosynthetic bacterial 6-phytase in enhancing phosphorus availability to weaned piglets fed a corn-soybean meal-based diet without added inorganic phosphate

Dersjant-Li, Y.; Villca, Boris; Sewalt, Vincent; Kreij, Arno de; Marchal, Luc; Velayudhan, Deepak; Sorg, Robin A.; Christensen, Trine; Mejldal, Rie; Nikolaev, Igor; Pricelius, Sina; Kim, Hyesook; Haaning, Svend; Sørensen, Jens F.; Lizardo, R.

doi:10.1016/j.aninu.2019.11.003

Cited by 20 publications

(7 citation statements)

References 14 publications

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“…Meanwhile, recent studies also have demonstrated that Phy could improve apparent total tract digestibility of Ca and P (Ca/P) in piglets' diets which had adequate or reduced Ca/P content. 10,11 Furthermore, supplementation with 500-4000 FTU kg −1 Phy had a beneficial effect on piglets, including increased P digestibility, average daily gain and growth performance. [12][13][14][15] Ca/P plays an important role in the skeletal bone mineralization in piglets.…”

Section: Introductionmentioning

confidence: 97%

“…The improvements in P utilization which could be achieved with supplemental Phy in pig diets have already been extensively reported. Meanwhile, recent studies also have demonstrated that Phy could improve apparent total tract digestibility of Ca and P (Ca/P) in piglets' diets which had adequate or reduced Ca/P content 10,11 . Furthermore, supplementation with 500–4000 FTU kg −1 Phy had a beneficial effect on piglets, including increased P digestibility, average daily gain and growth performance 12‐15 .…”

Section: Introductionmentioning

confidence: 99%

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Effects of phytase and 25‐hydroxyvitamin D3 supplementation on growth performance and bone development in weaned piglets in Ca‐ and P‐deficient dietary

et al. 2021

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BACKGROUND:The beneficial function of phytase and 25-hydroxyvitamin D3 (HyD) on the feed utilization rate has been widely investigated. However, studies concerning its influence on weaned piglets largely lag behind. The aim of this study was to investigate the effects of phytase and HyD supplementation on the growth performance and bone development in weaned piglets under dietary Ca and P deficiency. RESULTS:The results showed that dietary Ca and P deficiency decreased (P < 0.05) the content of serum P in 6-10 kg piglets, as well as reducing (P < 0.05) the contents of serum Ca and P, average daily gain (ADG), bone mineral density (BMD), breaking force (BF), bone ash and femur Ca in 10-20 kg piglets. Compared with the control group, the feed-to-gain ratio (F/G) of 6-10 kg piglets in the Phy group was decreased (P < 0.05), whereas the ADG, blood Ca and P, BMD, BF, bone ash, P apparent digestibility, Ca and P retention rate of 10-20 kg piglets were increased (P < 0.05). The contents of serum osteocalcin and HyD in 6-10 kg piglets and ADG were higher than in the control group (P < 0.05), as well as the contents of serum Ca and HyD in 10-20 kg piglets in the HyD treatment group. Supplementation with both Phy and HyD decreased the F/D (P < 0.05) and increased the contents of serum Ca, P and HyD in 6-10 kg piglets as well as enhancing the ADG, BMD, BF, bone ash, femur Ca and P, serum Ca and P, HyD, and the apparent digestibility and retention of Ca and P (P < 0.05) in 10-20 kg piglets. Supplementation with Phy and HyD in Ca-and P-deficient dietary decreased bone resorption, and improved tight arrangement of collagen fibers and oblique fibers in weaned piglets. CONCLUSION: These data indicated that supplementation with both 1500 U kg −1 Phy and 50 ∼g kg −1 HyD could enhance dietary Ca and P utilization and promote bone development in low Ca and P dietary, and supplementation with both Phy and HyD had a significant synergy effect compared to single supplement.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Effects of phytase and 25‐hydroxyvitamin D3 supplementation on growth performance and bone development in weaned piglets in Ca‐ and P‐deficient dietary

et al. 2021

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“…Phytic acid has been described as an antinutritional factor because monogastric animals lack endogenous enzymes to degrade it. At a pH above 4, phytic acid would be bound to cationic minerals such as Ca, zinc (Zn), and copper (Cu) resulting in the formation of phytate complexes that are indigestible ( Dersjant-Li et al, 2020 ). Phytic acid can also bind to AA and enzymes at pH levels above or below their isoelectric point, forming binary and ternary protein–complexes that are resistant to proteolytic enzyme hydrolysis ( Deshpande and Cheryan, 1984 ).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of a bacterial 6-phytase supplemented beyond traditional dose levels on jejunal mucosa-associated microbiota, ileal nutrient digestibility, bone parameters, and intestinal health, and growth performance of nursery pigs

Moita

Kim

2023

Journal of Animal Science

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This study aimed to determine the efficacy of a bacterial 6-phytase (Buttiauxella spp.) supplemented beyond traditional dose levels based on jejunal mucosa-associated microbiota, apparent ileal digestibility (AID), intestinal health and bone parameters, and growth performance of nursery pigs. Seventy-two weaned pigs (36 barrows and 36 gilts at 21 d of age with 5.8 ± 0.5 kg BW) were allotted to six treatments based on randomized complete block design with sex and initial BW as blocks and fed in three dietary phases (phase 1 for 14 d, phase 2 for 10 d and phase 3 for 14 d). The treatments included a negative control (NC) diet without phytase formulated meeting nutrient requirements by NRC and the other five treatments deficient in calcium (Ca) and phosphorus (P) by 0.12% with increasing levels of a bacterial 6-phytase (0, 500, 1,000, 2,000, and 5,000 FTU/kg feed). Titanium dioxide (0.4%) was added to phase 3 diets as an indigestible marker to measure AID of nutrients. On d 45, all pigs were euthanized to collect ileal digesta to measure AID, the third metacarpus to measure bone parameters, and jejunal mucosa to evaluate intestinal health and microbiota. Data were analyzed using the MIXED procedure for polynomial contrasts and the NLMIXED procedure for broken line analysis using the SAS 9.4. Broken line analysis demonstrated that 948 FTU/kg feed increased (P < 0.05) the ADG and the bone P content. Increasing phytase supplementation increased (linear, P < 0.05) AID of CP, bone P and ash content. Increasing phytase supplementation reduced (P < 0.05) the fecal score during phases 2 and 3. Broken line analysis demonstrated that 1,889 FTU/kg feed increased (P < 0.05) bone breaking strength. Increasing phytase supplementation (PC vs. Phy) increased (P < 0.05) AID of ether extract (EE) and P. The supplementation of phytase at 2,000 FTU/ kg feed tended (P = 0.087) to reduce the relative abundance of Prevotellaceae. In conclusion, the supplementation of a bacterial 6-phytase beyond traditional dose levels improved bone breaking strength, bone ash and P content, AID of CP, EE, and P, and growth performance of nursery pigs with reduced relative abundance of Bacteroidetes specifically Prevotellaceae in the jejunal mucosa. Supplementation of a bacterial 6-phytase between 1,000 and 2,000 FTU/kg feed provided benefits associated with growth performance and bone parameters of nursery pigs.

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“…A recent study has shown that a high dose (4,000 FTU/kg) of a phytase derived from Citrobacter braakii was able to ameliorate the negative effect of total removal of Pi from a Ca-reduced diet on broiler growth performance and bone conformation in grower and finisher phases ( Ribeiro et al., 2019 ). In pigs, whose digestible-P requirement is lower than that of young broilers ( NRC 1994 , 2012 ), a novel consensus bacterial 6-phytase with high capacity to degrade phytate was recently shown to be effective as a total replacement for inorganic-P during 40 to 70 d of age when applied at a dose-level of 1,000 FTU/kg to a corn-soybean meal–based diet containing a commercially relevant phytate-P content ( Dersjant-Li et al., 2020a ). It was estimated that the same phytase at the same dose level in broilers could replace 2.07 g MCP-P per kilogram of diet based on digestible-P improvement in a comparable diet with similar phytate-P content, maintaining performance and tibia ash during all growth phases equivalent to a nutritionally adequate control diet ( Dersjant-Li et al., 2020b ).…”

Section: Introductionmentioning

confidence: 99%

A novel consensus bacterial 6-phytase variant completely replaced inorganic phosphate in broiler diets, maintaining growth performance and bone quality: data from two independent trials

et al. 2021

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Total replacement of dietary inorganic phosphate ( Pi ) by a novel consensus bacterial 6-phytase variant ( PhyG ) in phytate-rich diets (>0.3% phytate-P) was investigated in 2 trials using growth performance and bone quality as outcome measures. Both trials utilized a completely randomized design with 5 dietary treatments across 4 phases: starter (0–10 d), grower (10–21 d), finisher 1 (21–35 d), and finisher 2 (35–42 d). Treatments comprised a nutritionally adequate positive control ( PC ) diet containing monocalcium phosphate and 4 experimental diets (IPF1, IPF2, IPF3, and IPF4), all containing no added Pi and reduced in Ca by 0.2 to 0.3% units vs. PC. IPF1contained PhyG at 1,000 FTU/kg (all phases); IPF2 contained PhyG at 1,000 FTU/kg (all phases) and was additionally reduced in digestible AA, ME, and sodium (−0.2 to −0.4% points, −74 kcal/kg, –0.04% points, respectively, vs. PC); IPF3 contained PhyG at 3,000 FTU/kg in starter, 2,000 FTU/kg in grower, and 1,000 FTU/kg in finisher phases; and IPF4 contained xylanase (2,000 U/kg) and PhyG (2,000 FTU/kg in starter, 1,500 FTU/kg in grower, and 1,000 FTU/kg in finisher phases) and was additionally reduced in ME (–71 kcal/kg vs. PC). Ross 308 broilers were used (trial 1: n = 1,200 mixed sex; 24 birds per pen × 10 replicates; trial 2: n = 1,300 males; 26 birds × 10 replicates). During all phases in both trials, all IPF treatments maintained or improved BW, ADG, ADFI, FCR and BW-corrected FCRc and bone quality parameters vs. PC. vs. PC, treatment IPF3 increased ADG during starter phase (+10.8%) and reduced overall FCRc (−12 points, P < 0.05) in Trial 1, and increased overall ADG (+4.4%), day 35 and day 42 BW (+3.5%, +4.9%), and reduced overall FCRc (−11 points) in Trial 2 ( P < 0.05). IPF4 produced equivalent performance to IPF3 (both trials). These are the first data to demonstrate total replacement of Pi by microbial phytase during an entire growth cycle in broiler diets.

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Functionality of a next generation biosynthetic bacterial 6-phytase in enhancing phosphorus availability to weaned piglets fed a corn-soybean meal-based diet without added inorganic phosphate

Cited by 20 publications

References 14 publications

Effects of phytase and 25‐hydroxyvitamin D3 supplementation on growth performance and bone development in weaned piglets in Ca‐ and P‐deficient dietary

Effects of phytase and 25‐hydroxyvitamin D3 supplementation on growth performance and bone development in weaned piglets in Ca‐ and P‐deficient dietary

Efficacy of a bacterial 6-phytase supplemented beyond traditional dose levels on jejunal mucosa-associated microbiota, ileal nutrient digestibility, bone parameters, and intestinal health, and growth performance of nursery pigs

A novel consensus bacterial 6-phytase variant completely replaced inorganic phosphate in broiler diets, maintaining growth performance and bone quality: data from two independent trials

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