Functional substitution of the recE gene of Bacillus subtilis by the recA gene of Proteus mirabilis

Eitner, G.; Manteuffel, Renate; Hofemeister, J.

doi:10.1007/bf00341456

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…The recE4 mutant strains of B. subtilis were the most UV sensitive and were depressed at least 1,000-fold in transformation frequency (7). Recently, deVos and Venema (3) detected a 45,000-dalton protein, missing in recE4 mutants, that has been shown to be induced by UV irradiation or mitomycin C. The B. subtilis recE4 gene can be complemented by the recA genes of E. coli (2) and Proteus mirabilis (8). Functional similarities between the recF and recG genes of B. subtilis and the E. coli recL gene have also been found (4).…”

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confidence: 99%

Isolation of recombination-defective and UV-sensitive mutants of Bacillus megaterium

English¹,

Vary²

1986

J Bacteriol

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Mutants of Bacillus megaterium QMB1551 sensitive to mitomycin C or methyl methanesulfonate were isolated and characterized phenotypically. Cell survival after UV-light and gamma-ray exposure was determined, as was transductional recombination. Of the mutants tested, three were sensitive to UV but remained recombination proficient. The UV-sensitive mutants were also reduced in host cell reactivation. At least three mutants had undetectable transduction frequencies, i.e., less than 0.3 to 1.3% of the parental strain frequencies, and so appear to be recombination deficient. Sensitivities of these mutant strains to UV light and gamma radiation were compared with those of parental B. megaterium as well as parental, recE4, recA1, uvrA19, and uvrB109 strains of Bacillus subtilis. In each case, the strains of B. megaterium, including the parental strains, showed a higher percentage of cell survival than B. subtilis.

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confidence: 99%

Isolation of recombination-defective and UV-sensitive mutants of Bacillus megaterium

English¹,

Vary²

1986

J Bacteriol

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“…Another recombinant recApm plasmid, pHP334, previously used for expression of recApm in B. subti& ( EITNER et al 1984), expressed the same amount of RecA protein as the pPL603recApm plasmids (results not shown). This was indicative of the use of the recApm promoter in pHP334.…”

Section: U V and Cm Resistancementioning

confidence: 93%

“…We previously presented results which strongly suggested expression and biological function in B. subtilis of the recA gene of the gramnegative P . mirabilis (EITNER et al 1984). In the present paper we prove that expression of the P .…”

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confidence: 99%

Expression of the Proteus mirabilis recA gene in Bacillus subtilis is directed by its own promoter

Eitner‐mönke¹,

Manteuffel²

1986

J. Basic Microbiol.

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The recA gene of Proteus mirabilis (recApm) has been cloned into the PstI site of the Bacillus promoter-probe plasmid pPL603. When present on this plasmid, the recApm1) gene is expressed in B. subtilis under the control of its own transcriptional and translational signals. It is concluded that the high AT-content of the DNA sequence upstream of the -35 region is of decisive importance for the usage of the recApm promoter by the B. subtilis RNA polymerase. The results are discussed in relation to the expression barriers found to exist for genes from gram-negative bacteria in the gram-positive B. subtilis.

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“…Analysis of the "The novAB marker is a low-efficiency (0.1) marker. 6 Plasmid prec2255 transferred to a fresh Rd1388 culture.…”

Section: Methodsmentioning

confidence: 99%

Cloning and characterization of the Haemophilus influenzae Rd rec-1+ gene

Stuy

1989

J Bacteriol

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The Haemophilus influenzae Rd rec-l+ gene was cloned from a partial chromosomal digest into a plasmid vector as a 20-kilobase-pair (kbp) BstEII fragment and then subcloned. The smallest subclone with rec-l+ activity carried a 3.1-kbp EcoRI fragment. The identity of the rec-l gene in these clones was confirmed by transforming an Rd strain carrying a leaky rec-l mutation (recA4) to resistance to methyl methanesulfonate (MMS) by using whole or

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Functional substitution of the recE gene of Bacillus subtilis by the recA gene of Proteus mirabilis

Cited by 5 publications

References 42 publications

Isolation of recombination-defective and UV-sensitive mutants of Bacillus megaterium

Isolation of recombination-defective and UV-sensitive mutants of Bacillus megaterium

Expression of the Proteus mirabilis recA gene in Bacillus subtilis is directed by its own promoter

Cloning and characterization of the Haemophilus influenzae Rd rec-1+ gene

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