Functional state of the liver antioxidant system of the bream Abramis brama (L.) from Rybinsk Reservoir regions with different anthropogenic loads

Abstract

The breams living in five regions of the Rybinsk Reservoir with different degrees of anthropogenic load have been assayed for the characteristics of their antioxidant system (AOS) and lipid peroxidation (LPO) system. Individuals caught at two stations in the Rybinsk Reservoir that maximally differ in the degree of pol lution display different qualitative and quantitative compositions of polychlorinated biphenyls (PCBs). At the station at the Sheksninskii Reach, which is located near a local source of pollution (Cherepovets), the total content of PCBs and homologous groups of congeners is one order of magnitude higher than in the bream living at the station of the comparatively clean Molozhskii Reach. The AOS response and the generation intensity of LPO products in the bream (Abramis brama L.) liver caused by different degrees of anthropogenic pollution have been determined. The content of LPO products in the liver of the breams caught in the Shek sninskii Reach is considerably higher and the values of AOS characteristics are considerably lower than in the fish from the Molozhskii Reach. The bream living in the Molozhskii and Volzhskii Reaches are in a more favorable state than those in the Sheksninskii Reach. The AOS and LPO characteristics are an appropriate biomarker for estimating the state of the health of the fish.

Effect of sodium chloride on oxidative stress biomarkers of the freshwater bivalve Anodonta cygnea

“…Тенденция повышения уровня GSH обнаружена у M. galloprovincialis на загрязненных станциях в естественной среде, в результате накопления в моллюсках Cr, Fe и Mn (Regoli et al, 2004). В других работах на моллюсках M. galloprovincialis и рыбах Abramis brama и Dicentrarchus labrax отмечено уменьшение концентрации GSH в результате влияния тяжелых металлов и СОЗ в полевых и лабораторных условиях (Canesi et al, 1999;Morozov et al, 2012). В некоторых исследованиях сообщалось о кратковременном повышении концентрации GSH и последующем уменьшении у разных видов растений и животных.…”

“…Концентрацию белка в пробах измеряли методом Брэдфорд с бычьим сывороточным альбумином в качестве стандарта. Температура инкубационной среды при определении активности ферментов поддерживалась на уровне 25,0 ± 0,5 °С (Morozov et al, 2012).…”

Responses of hepatic biochemical markers in bream Abramis brama L. to dietary administered polychlorinated biphenyls

“…Production of PCBs is currently discontinued; however, they still circulate in ecosystems at high con centrations owing to stability in the environment and the capacity for bioaccumulation (Mamontov et al, 2000;Chuiko et al, 2010;Morozov et al, 2012;etc.). Waste dumps containing PCB contaminated waste, as well as old equipment, are sources of PCB leakage into the environment (Harrad et al, 1994).…”