Functional silica nanospheres for sensitive detection of H9N2 avian influenza virus based on immunomagnetic separation

Luo, Fanwei; Long, Chao; Wu, Zhen; Xiong, Huayu; Chen, Miaomiao; Zhang, Xun; Wen, Wei

doi:10.1016/j.snb.2020.127831

Cited by 24 publications

(15 citation statements)

References 45 publications

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“… (1) Difficult to prepare; (2) Easy to aggregate. ( Kholafazad Kordasht et al, 2020 ; Luo et al, 2020a ; Vandghanooni et al, 2020 ; Zhou et al, 2014 ) Gold Nanoparticles (AuNPs) (1) Biocompatible; (2) Wide size distribution (1–150 nm); (3) Photoelectric effect with size and morphology dependence; (4) Strong covalent bond with thiol; (5) Electric conductive. (1) High sensitivity; (2) Rapid response; (3) Rich surface active sites; (4) Strong adsorption.…”

Section: Viral Nucleic Acid Detection Based On Mofmentioning

confidence: 99%

Metal-organic frameworks for virus detection

Wang

et al. 2020

Biosensors and Bioelectronics

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Virus severely endangers human life and health, and the detection of viruses is essential for the prevention and treatment of associated diseases. Metal-organic framework (MOF), a novel hybrid porous material which is bridged by the metal clusters and organic linkers, has become a promising biosensor platform for virus detection due to its outstanding properties including high surface area, adjustable pore size, easy modification, etc. However, the MOF-based sensing platforms for virus detection are rarely summarized. This review systematically divided the detection platforms into nucleic acid and immunological (antigen and antibody) detection, and the underlying sensing mechanisms were interpreted. The nucleic acid sensing was discussed based on the properties of MOF (such as metal ion, functional group, geometry structure, size, porosity, stability, etc.), revealing the relationship between the sensing performance and properties of MOF. Moreover, antibodies sensing based on the fluorescence detection and antigens sensing based on molecular imprinting or electrochemical immunoassay were highlighted. Furthermore, the remaining challenges and future development of MOF for virus detection were further discussed and proposed. This review will provide valuable references for the construction of sophisticated sensing platform for the detection of viruses, especially the 2019 coronavirus.

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Section: Viral Nucleic Acid Detection Based On Mofmentioning

confidence: 99%

Metal-organic frameworks for virus detection

Wang

et al. 2020

Biosensors and Bioelectronics

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“…As expected, a positive response was observed above a detection limit of 3 × 10 6 PFU mL −1 . MELISA shows good sensitivity for H1N1 and H3N2 detection but lacks sensitivity compared with the other immunomagnetic bead-based assays described in the literature [20,34,35]. The negative responses obtained for non-related PRRSV at different concentrations indicate the selectivity of MELISA for Influenza A viruses (Fig.…”

Section: Melisa Detection Of H1n1 and H2n3 Virusesmentioning

confidence: 91%

Specific and sensitive detection of Influenza A virus using a biotin-coated nanoparticle enhanced immunomagnetic assay

et al. 2020

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This study reports the development of a sensitive magnetic bead-based enzyme-linked immunoassay (MELISA) for the panreactive detection of the Influenza A virus. The assay combines immunomagnetic beads and biotin-nanoparticle-based detection to quantify a highly conserved viral nucleoprotein in virus lysates. At the capture step, monoclonal antibody-coated magnetic microbeads were used to bind and concentrate the nucleoprotein in samples. The colorimetric detection signal was amplified using biotinylated silica nanoparticles (NP). These nanoparticles were functionalized on the surface with short DNA spacers bearing biotin groups by an automated supported synthesis method performed on nano-on-micro assemblies with a DNA/RNA synthesizer. A biotin-nanoparticle and immunomagnetic bead-based assay was developed. We succeeded in detecting Influenza A viruses directly in the lysis buffer supplemented with 10% saliva to simulate the clinical context. The biotin-nanoparticle amplification step enabled detection limits as low as 3 × 10 3 PFU mL −1 and 4 × 10 4 PFU mL −1 to be achieved for the H1N1 and H3N2 strains respectively. In contrast, a classical ELISA test based on the same antibody sandwich showed detection limit of 1.2 × 10 7 PFU mL −1 for H1N1. The new enhanced MELISA proved to be specific, as no cross-reactivity was found with a porcine respiratory virus (PRRSV).

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“…According to previous research, nanotechnology has been considered in the process of diagnosis and treatment of many viral diseases. The use of luminescence electrochemical biosensors for the selective detection of avian influenza virus (H9N2-AIV) [17] and the use of magnetic nanoparticles (NPs) for the detection of influenza A virus subtype H1N1 virus [18] are examples of nanoparticles applications in virus detection. The use of poly vinyl pyrrolidone (PVP) coated AgNPs (30-50 nm) on HIV strains has also been shown to have antiviral activity [19].…”

Section: Application Of Nanotechnology In the Detection And Treatment Of Viral Infectionsmentioning

confidence: 99%

Use of nanotechnology in the diagnosis and treatment of coronavirus

et al. 2021

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Coronavirus is a beta virus that has caused a worldwide pandemic since December 2019. Many treatments such as antiviral drugs, immunosuppressive drugs, neutralizing antibodies, and monoclonal antibodies have been tested on coronavirus disease 2019 (COVID-19) that most of them were effective. Given that nanotechnology-based approaches have been successful in detection and treatment of viral systems such as human immunodeficiency virus (HIV), influenza A virus subtype H1N1 and Middle East respiratory syndrome coronavirus (MERS-CoV), they also seem to be effective in detecting and treating COVID-19. Nanotechnology is used in various methods for early and rapid diagnosis of the disease. Nanoparticles can be used in products for the diagnosis, treatment and prevention of COVID-19. These substances are very effective in the controlled delivery of antiviral drugs and biomolecules and they are also used in the manufacture of personal safety equipment, widely, and the production of anti-virus coatings for surfaces, air filters and the production of vaccines. In general, nanomaterial can play an important role in controlling the disease, based on strategies to prevent the virus from entering the host cell, inhibiting virus replication, virus delivery systems, and nano-based vaccines. Nanotechnology is a multidisciplinary tool that can offer a variety of solutions based on disease prevention, diagnosis and treatment strategies.

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Functional silica nanospheres for sensitive detection of H9N2 avian influenza virus based on immunomagnetic separation

Cited by 24 publications

References 45 publications

Metal-organic frameworks for virus detection

Metal-organic frameworks for virus detection

Specific and sensitive detection of Influenza A virus using a biotin-coated nanoparticle enhanced immunomagnetic assay

Use of nanotechnology in the diagnosis and treatment of coronavirus

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