Functional reservoir microcapsules generated <i>via</i> microfluidic fabrication for long-term cardiovascular therapeutics

Dinh, Ngoc-Duy; Kukumberg, Marek; Nguyen, Anh-Tuan; Keramati, Hamed; Guo, Song; Phan, Dinh‐Tuan; Jaafar, Nurdiyana Binte; Birgersson, Erik; Leo, Hwa Liang; Huang, Ruby Yun‐Ju; Kofidis, Theo; Rufaihah, Abdul Jalil; Chen, Chia-Hung

doi:10.1039/d0lc00296h

Cited by 27 publications

(25 citation statements)

References 31 publications

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“…The middle phase (shell) can be selectively gelled or hardened to create solid capsules, , and the minimized oil volume prevents the oil from diffusing to and affecting the inner content directly . Moreover, unlike water-in-oil (w/o) single emulsion, DE is compatible with commercial flow cytometers (FC) and fluorescence-activated cell sorting (FACS) instruments that require an aqueous carrier flow, allowing user-friendly quantitative high-throughput assays. − Due to these significant advantages, DE has been widely used in various food, cosmetic, pharmaceutical, chemical, and biological applications, such as the encapsulation and release of nutrients and flavors, production of low-calorie food, controlled release and targeted delivery of drugs, chemical extraction, synthesis of functional particles, medical diagnostics, and directed cellular and molecular evolution. ,− …”

Section: Introductionmentioning

confidence: 99%

Rapid, Simple, and Inexpensive Spatial Patterning of Wettability in Microfluidic Devices for Double Emulsion Generation

Liu

Piper

2021

Anal. Chem.

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Water-in-oil-in-water (w/o/w) double emulsion (DE) encapsulation has been widely used as a promising platform technology for various applications in the fields of food, cosmetics, pharmacy, chemical engineering, materials science, and synthetic biology. Unfortunately, DEs formed by conventional emulsion generation approaches in most cases are highly polydisperse, making them less desirable for quantitative assays, controlled biomaterial synthesis, and entrapped ingredient release. Microfluidic devices can generate monodisperse DEs with controllable size, morphology, and production rate, but these generally require multistep fabrication processes and use of different solvents or bulky external instrumentation to pattern channel wettability. To overcome these limitations, we propose a rapid, simple, and inexpensive method to spatially pattern wettability in microfluidic devices for the continuous generation of monodisperse DEs. This is achieved by applying corona−plasma treatment to a select zone of the microchannel surface aided by a custom-designed corona resistance microchannel to strictly confine the plasma-treatment zone in a single polydimethylsiloxane (PDMS) microfluidic device. The properties of PDMS channel surfaces and key microchannel regions for DE generation are characterized under different levels of treatment. The size, shell thickness, and number of inner cores of generated DEs are shown to be highly controllable by tuning the phase flow rate ratios. Using DEs as templates, we successfully achieve a one-step generation and collection of gelatin microgels. Additionally, we demonstrate the biological capability of generated DEs by flow cytometric screening of the encapsulation and growth of yeast cells within DEs. We expect that the proposed approach will be widely used to create microfluidic devices with more complex wettability patterns.

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Section: Introductionmentioning

confidence: 99%

Rapid, Simple, and Inexpensive Spatial Patterning of Wettability in Microfluidic Devices for Double Emulsion Generation

Liu

Piper

2021

Anal. Chem.

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“…In the acute myocardial infarction model of a rat, drugs like VEGF and PDGF have been delivered through this method. It was found that the repair of the infraction was significant with respect to the reduction in ventricular wall thickness and fibrosis, and the percentage of scar tissue was reduced to 11.2%, compared to 21.4% with saline [212].…”

Section: Drug Discovery and Disease Modellingmentioning

confidence: 97%

Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction

Sharma

Dash

Govarthanan

et al. 2021

Cells

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Myocardium Infarction (MI) is one of the foremost cardiovascular diseases (CVDs) causing death worldwide, and its case numbers are expected to continuously increase in the coming years. Pharmacological interventions have not been at the forefront in ameliorating MI-related morbidity and mortality. Stem cell-based tissue engineering approaches have been extensively explored for their regenerative potential in the infarcted myocardium. Recent studies on microfluidic devices employing stem cells under laboratory set-up have revealed meticulous events pertaining to the pathophysiology of MI occurring at the infarcted site. This discovery also underpins the appropriate conditions in the niche for differentiating stem cells into mature cardiomyocyte-like cells and leads to engineering of the scaffold via mimicking of native cardiac physiological conditions. However, the mode of stem cell-loaded engineered scaffolds delivered to the site of infarction is still a challenging mission, and yet to be translated to the clinical setting. In this review, we have elucidated the various strategies developed using a hydrogel-based system both as encapsulated stem cells and as biocompatible patches loaded with cells and applied at the site of infarction.

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“…Dinh et al. [ 87 ] prepared a liquid core and polymer shell by using microfluidic technology to separate polyethylene glycol diacrylate, biocompatible polymer, and dextran in droplets. The dextran spontaneously separated from the polyethylene glycol diacrylate in the droplet to form a liquid core, while the polyethylene glycol diacrylate remained in the shell and finally polymerized via ultraviolet irradiation.…”

Section: Drug Deliverymentioning

confidence: 99%

Injectable Microfluidic Hydrogel Microspheres for Cell and Drug Delivery

Zhao

Wang

et al. 2021

Adv Funct Materials

160

107

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Microfluidic hydrogel microspheres have been broadly studied across a wide range of industries and applications, and their use in the medical field, including control cells and drug delivery, is increasing. The usual design of these materials is intended to enable the efficient and smart encapsulation of cells and/or drugs in microspheres in which the functionalities and features are effectively controlled, lending itself some unique properties. These characteristics promote exchanges and cooperation in multiple disciplines and boost the development of precision medicine, new manufacturing technologies, and applied materials. This review begins with a discussion of microfluidic hydrogel microspheres and then introduces the preparation equipment, main principles, and related characteristics of the microspheres. Furthermore, the medical applications of microfluidic hydrogel microspheres for delivering cells and drugs are emphasized. Finally, this review discusses perspectives and future directions for accelerating the development and application of microfluidic hydrogel microspheres for controlled delivery.

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Functional reservoir microcapsules generated via microfluidic fabrication for long-term cardiovascular therapeutics

Abstract:
The reservoir microcapsules are fabricated through one-step microfluidic phase separation for long-term cardiovascular therapeutics.

Cited by 27 publications

References 31 publications

Rapid, Simple, and Inexpensive Spatial Patterning of Wettability in Microfluidic Devices for Double Emulsion Generation

Rapid, Simple, and Inexpensive Spatial Patterning of Wettability in Microfluidic Devices for Double Emulsion Generation

Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction

Injectable Microfluidic Hydrogel Microspheres for Cell and Drug Delivery

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Functional reservoir microcapsules generated via microfluidic fabrication for long-term cardiovascular therapeutics

Abstract: The reservoir microcapsules are fabricated through one-step microfluidic phase separation for long-term cardiovascular therapeutics.

Cited by 27 publications

References 31 publications

Rapid, Simple, and Inexpensive Spatial Patterning of Wettability in Microfluidic Devices for Double Emulsion Generation

Rapid, Simple, and Inexpensive Spatial Patterning of Wettability in Microfluidic Devices for Double Emulsion Generation

Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction

Injectable Microfluidic Hydrogel Microspheres for Cell and Drug Delivery

Contact Info

Product

Resources

About

Abstract:
The reservoir microcapsules are fabricated through one-step microfluidic phase separation for long-term cardiovascular therapeutics.