Functional redundancy and divergence of β-carbonic anhydrases in Physcomitrella patens

Chen, Zexi; Wang, Wenbo; Dong, Xueyi; Pu, Xiaojun; Gao, Bei; Liu, Li

doi:10.1007/s00425-020-03429-8

Cited by 2 publications

(1 citation statement)

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“…We measured the chlorophyll fluorescence parameter, F v /F m , to evaluate the quantum yield of photosystem II (Figure 4b,c). This parameter is a sensitive reporter of the level of stress experienced by plants (Alboresi et al, 2010; Azzabi et al, 2012; Chen et al, 2020; Gao et al, 2016; Pettinari et al, 2022). We found that F v /F m of colonies grown on cellophane and NRP were statistically indistinguishable (Figure 4d), demonstrating that growth on NRP does not significantly impair photosynthesis.…”

Section: Resultsmentioning

confidence: 99%

A fibrous scaffold for in vitro culture and experimental studies of Physcomitrium patens

Calcutt,

Aghli,

Arinzeh

et al. 2024

Plant Direct

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The model moss, Physcomitrium patens, is routinely cultured on cellophane placed over a solid nutrient medium. While this culture method is convenient for moss propagation, it is not suitable for studying how topographical features and mechanical cues from the environment influence the growth and development of moss. Here, we show that P. patens can be grown on fibrous scaffolds consisting of nanoscale, randomly oriented fibers composed of polyvinylidene tri‐fluoroethylene (NRP). The moss adheres tightly to NRP in contrast to the lack of adhesion to cellophane. Adhesion to the scaffold is associated with slower tip growth of moss protonema for some time, followed by an increase in tip growth rate that is equivalent to that on cellophane. In addition, the orientation of the first subapical cell division plane differs between NRP‐grown and cellophane‐grown protonema. Nonetheless, moss colonies grown on NRP did not show signs of nutrient or photosynthetic stress and developed normal gametophores. Together, these data establish NRP as a suitable substrate for the culture of P. patens and to probe the influence of mechanical forces on tip growth and cell division of moss.

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Section: Resultsmentioning

confidence: 99%

A fibrous scaffold for in vitro culture and experimental studies of Physcomitrium patens

Calcutt,

Aghli,

Arinzeh

et al. 2024

Plant Direct

View full text Add to dashboard Cite

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Identification and molecular characterization of the alternative spliced variants of beta carbonic anhydrase 1 (βCA1) from Arabidopsis thaliana

Shen

et al. 2021

PeerJ

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Carbonic anhydrases (CAs) are ubiquitous zinc metalloenzymes that catalyze the interconversion of carbon dioxide and bicarbonate. Higher plants mainly contain the three evolutionarily distinct CA families αCA, βCA, and γCA, with each represented by multiple isoforms. Alternative splicing (AS) of the CA transcripts is common. However, there is little information on the spliced variants of individual CA isoforms. In this study, we focused on the characterization of spliced variants of βCA1 from Arabidopsis. The expression patterns and subcellular localization of the individual spliced variants of βCA1 were examined. The results showed that the spliced variants of βCA1 possessed different subcellular and tissue distributions and responded differently to environmental stimuli. Additionally, we addressed the physiological role of βCA1 in heat stress response and its protein-protein interaction (PPI) network. Our results showed that βCA1 was regulated by heat stresses, and βca1 mutant was hypersensitive to heat stress, indicating a role for βCA1 in heat stress response. Furthermore, PPI network analysis revealed that βCA1 interacts with multiple proteins involved in several processes, including photosynthesis, metabolism, and the stress response, and these will provide new avenues for future investigations of βCA1.

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Functional redundancy and divergence of β-carbonic anhydrases in Physcomitrella patens

Abstract: Main conclusion β-carbonic anhydrases, which function in regulating plant growth, C/N status, and stomata number, showed functional redundancy and divergence in Physcomitrella patens.

Cited by 2 publications

References 46 publications

A fibrous scaffold for in vitro culture and experimental studies of Physcomitrium patens

A fibrous scaffold for in vitro culture and experimental studies of Physcomitrium patens

Identification and molecular characterization of the alternative spliced variants of beta carbonic anhydrase 1 (βCA1) from Arabidopsis thaliana

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